Fine structure of the chorion of Manduca sexta and Sesamía nonagrioides as revealed by scanning electron microscopy and freeze-fracturing

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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Fibroblasts During Hypertrophic Scar Formation and Resolution

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072587 ◽

1973 ◽

Vol 31 ◽

pp. 428-429

Author(s):

C. W. Kischer

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Cell Proliferation ◽

Fine Structure ◽

Metabolic Activity ◽

Hypertrophic Scar ◽

Normal Skin ◽

Ground Substance ◽

Hypertrophic Scars ◽

Scanning Electron

The morphology of the fibroblasts changes markedly as the healing period from burn wounds progresses, through development of the hypertrophic scar, to resolution of the scar by a self-limiting process of maturation or therapeutic resolution. In addition, hypertrophic scars contain an increased cell proliferation largely made up of fibroblasts. This tremendous population of fibroblasts seems congruous with the abundance of collagen and ground substance. The fine structure of these cells should reflect some aspects of the metabolic activity necessary for production of the scar, and might presage the stage of maturation.A comparison of the fine structure of the fibroblasts from normal skin, different scar types, and granulation tissue has been made by transmission (TEM) and scanning electron microscopy (SEM).

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Ultrastructure of the soil fungus, Geomyces pannorus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100113573 ◽

1984 ◽

Vol 42 ◽

pp. 738-739

Author(s):

J. A. Traquair ◽

E. G. Kokko

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Fine Structure ◽

Low Temperatures ◽

Soil Fungus ◽

Organic Soils ◽

Anatomical Studies ◽

Transmission Electron ◽

Electron Microscopical ◽

Scanning Electron

With the advent of improved dehydration techniques, scanning electron microscopy has become routine in anatomical studies of fungi. Fine structure of hyphae and spore surfaces has been illustrated for many hyphomycetes, and yet, the ultrastructure of the ubiquitous soil fungus, Geomyces pannorus (Link) Sigler & Carmichael has been neglected. This presentation shows that scanning and transmission electron microscopical data must be correlated in resolving septal structure and conidial release in G. pannorus.Although it is reported to be cellulolytic but not keratinolytic, G. pannorus is found on human skin, animals, birds, mushrooms, dung, roots, and frozen meat in addition to various organic soils. In fact, it readily adapts to growth at low temperatures.

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Stereoscopic Examination of the Fine Structure of Some Metal Coatings Used for Scanning Electron Microscopy / Stereoskopische Gefügeuntersuchung von in der Rasterelektronenmikroskopie verwendeten Metallbeschichtungen

Practical Metallography ◽

10.1515/pm-1983-200106 ◽

1983 ◽

Vol 20 (1) ◽

pp. 31-41

Author(s):

Colin Beaton ◽

Barry Filshie

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Fine Structure ◽

Metal Coatings ◽

Scanning Electron

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Improving the Resolution of Sputter-coated Films

Microscopy Today ◽

10.1017/s1551929500057448 ◽

2000 ◽

Vol 8 (2) ◽

pp. 16-17

Author(s):

Mary Mager

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Fine Structure ◽

High Resolution ◽

Carbon Film ◽

Metal Films ◽

Pure Gold ◽

High Magnification ◽

Conducting Film ◽

Scanning Electron

After an inquiry from the Microscopy Listserver, I went back to my 1980 copy of Scanning Electron Microscopy, volume I. Several authors had investigated the structure of thin metal films by depositing the films onto carbon-film-covered TEM grids and imaging the films at high magnification. There were several proposals for new devices that have since become standards for high-resolution coaters, but the Listserver inquiry was for a fine conducting film suitabie for high-resolution SEM from an existing sputter coater.There were several factors studied that influenced the fine structure of the films. The first was the materials sputtered: for a given set of conditions of voltage, current and time, platinum gave the finest film, 60% gold-40% palladium (Au/Pd) the next finest and pure gold the least fine.

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