scholarly journals A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis

2011 ◽  
Vol 80 (2) ◽  
pp. 335-349 ◽  
Author(s):  
Natalia Martin ◽  
Quin H. Christensen ◽  
María C. Mansilla ◽  
John E. Cronan ◽  
Diego de Mendoza
Keyword(s):  
Bacillus Subtilis ◽  
Lipoic Acid ◽  
Acid Biosynthesis

scholarly journals Improved biotin, thiamine, and lipoic acid biosynthesis by engineering the global regulator IscR

2020 ◽  
Vol 60 ◽  
pp. 97-109
Author(s):  
Anne P. Bali ◽  
David Lennox-Hvenekilde ◽  
Nils Myling-Petersen ◽  
Josi Buerger ◽  
Bo Salomonsen ◽  
...  
Keyword(s):  
Lipoic Acid ◽  
Global Regulator ◽  
Acid Biosynthesis

scholarly journals Precise Deletion of tagD and Controlled Depletion of Its Product, Glycerol 3-Phosphate Cytidylyltransferase, Leads to Irregular Morphology and Lysis of Bacillus subtilisGrown at Physiological Temperature

2001 ◽  
Vol 183 (22) ◽  
pp. 6688-6693 ◽  
Author(s):  
Amit P. Bhavsar ◽  
Terry J. Beveridge ◽  
Eric D. Brown
Keyword(s):  
Bacillus Subtilis ◽  
Gene Product ◽  
Genetic Background ◽  
Expression System ◽  
Teichoic Acid ◽  
Structural Defects ◽  
Acid Biosynthesis ◽  
Physiological Temperature ◽  
Conditional Expression ◽  
Phenotypic Rescue

ABSTRACT Using a previously reported conditional expression system for use in Bacillus subtilis (A. P. Bhavsar, X. Zhao, and E. D. Brown, Appl. Environ. Microbiol. 67:403–410, 2001), we report the first precise deletion of a teichoic acid biosynthesis (tag) gene, tagD,in B. subtilis. This teichoic acid mutant showed a lethal phenotype when characterized at a physiological temperature and in a defined genetic background. This tagD mutant was subject to full phenotypic rescue upon expression of the complementing copy oftagD. Depletion of the tagD gene product (glycerol 3-phosphate cytidylyltransferase) via modulated expression of tagD from the amyE locus revealed structural defects centered on shape, septation, and division. Thickening of the wall and ultimately lysis followed these events.

Lipoic acid biosynthesis defects

2014 ◽  
Vol 37 (4) ◽  
pp. 553-563 ◽  
Author(s):  
Johannes A. Mayr ◽  
René G. Feichtinger ◽  
Frederic Tort ◽  
Antonia Ribes ◽  
Wolfgang Sperl
Keyword(s):  
Lipoic Acid ◽  
Acid Biosynthesis

scholarly journals Insufficient Expression of the ilv-leu Operon Encoding Enzymes of Branched-Chain Amino Acid Biosynthesis Limits Growth of a Bacillus subtilis ccpA Mutant

2002 ◽  
Vol 184 (18) ◽  
pp. 5174-5178 ◽  
Author(s):  
Holger Ludwig ◽  
Christoph Meinken ◽  
Anastasija Matin ◽  
Jörg Stülke
Keyword(s):  
Bacillus Subtilis ◽  
Amino Acid ◽  
Optimal Growth ◽  
Growth Defect ◽  
Acid Biosynthesis ◽  
Branched Chain Amino Acid ◽  
Mutant Strains ◽  
Regulatory Link ◽  
Branched Chain ◽  
Control Of Expression

ABSTRACT Bacillus subtilis ccpA mutant strains exhibit two distinct phenotypes: they are defective in catabolite repression, and their growth on minimal media is strongly impaired. This growth defect is largely due to a lack of expression of the gltAB operon. However, growth is impaired even in the presence of glutamate. Here, we demonstrate that the ccpA mutant strain needs methionine and the branched-chain amino acids for optimal growth. The control of expression of the ilv-leu operon by CcpA provides a novel regulatory link between carbon and amino acid metabolism.

Improvement of poly-γ-glutamic acid biosynthesis in a moving bed biofilm reactor by Bacillus subtilis NX-2

2016 ◽  
Vol 218 ◽  
pp. 360-366 ◽  
Author(s):  
Yongxiang Jiang ◽  
Bao Tang ◽  
Zongqi Xu ◽  
Kun Liu ◽  
Zheng Xu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Glutamic Acid ◽  
Biofilm Reactor ◽  
Moving Bed Biofilm Reactor ◽  
Acid Biosynthesis ◽  
Moving Bed
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