Association of measles virus with neurofibrillary tangles in subacute sclerosing panencephalitis: a combined in situ hybridization and immunocytochemical investigation

1994 ◽  
Vol 20 (2) ◽  
pp. 103-110 ◽  
Author(s):  
S. McQuaid ◽  
I. V. Allen ◽  
J. McMahon ◽  
J. Kirk
Keyword(s):  
In Situ Hybridization ◽  
Neurofibrillary Tangles ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Immunocytochemical Investigation

Subacute sclerosing panencephalitis: Measles virus matrix protein nucleic acid sequences detected by in situ hybridization

Neurology ◽  
1985 ◽  
Vol 35 (11) ◽  
pp. 1605-1605 ◽  
Author(s):  
P. Shapshak ◽  
W. W. Tourtellotte ◽  
S. Nakamura ◽  
M. C. Graves ◽  
M. Darvish ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Measles Virus ◽  
Matrix Protein ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Sequences

scholarly journals Cloning the Antibody Response in Humans with Chronic Inflammatory Disease: Immunopanning of Subacute Sclerosing Panencephalitis (SSPE) Brain Sections with Antibody Phage Libraries Prepared from SSPE Brain Enriches for Antibody Recognizing Measles Virus Antigens In Situ

2000 ◽  
Vol 74 (3) ◽  
pp. 1533-1537 ◽  
Author(s):  
Gregory P. Owens ◽  
R. Anthony Williamson ◽  
Mark P. Burgoon ◽  
Omar Ghausi ◽  
Dennis R. Burton ◽  
...  
Keyword(s):  
Causative Agent ◽  
Measles Virus ◽  
Inflammatory Diseases ◽  
Subacute Sclerosing Panencephalitis ◽  
Chronic Inflammatory Disease ◽  
Oligoclonal Bands ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Extracts ◽  
Antibody Phage

ABSTRACT In central nervous system (CNS) infectious and inflammatory diseases of known cause, oligoclonal bands represent antibody directed against the causative agent. To determine whether disease-relevant antibodies can be cloned from diseased brain, we prepared an antibody phage display library from the brain of a human with subacute sclerosing panencephalitis (SSPE), a chronic encephalitis caused by measles virus, and selected the library against SSPE brain sections. Antibodies that were retrieved reacted strongly with measles virus cell extracts by enzyme-linked immunosorbent assay and were specific for the measles virus nucleocapsid protein. These antibodies immunostained cells in different SSPE brains but not in control brain. Our data provide the first demonstration that diseased brain can be used to select in situ for antibodies directed against the causative agent of disease and point to the potential usefulness of this approach in identifying relevant antibodies in chronic CNS or systemic inflammatory diseases of unknown cause.

scholarly journals Measles Virus RNA Detected in Paget's Disease Bone Tissue by in situ Hybridization

1986 ◽  
Vol 67 (5) ◽  
pp. 907-913 ◽  
Author(s):  
M. F. Basle ◽  
J. G. Fournier ◽  
S. Rozenblatt ◽  
A. Rebel ◽  
M. Bouteille
Keyword(s):  
In Situ Hybridization ◽  
Bone Tissue ◽  
Measles Virus ◽  
Paget’S Disease ◽  
Paget's Disease ◽  
Virus Rna

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

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