First record of natural infection of Marlierea edulis by the eucalyptus canker fungus Chrysoporthe cubensis

Simultaneous-infection and serological tests demonstrate that a ringspot virus associated with a disease of Pelargonium hortorum cv. Mme. Salleron is related to tobacco ringspot virus. This report is believed to constitute the first record of natural infection of Pelargonium by this virus.

Download Full-text

Trypanosomatids in Phlebotomine Sand Flies (Diptera: Phlebotominae) From Anthropic and Sinantropic Landscapes in a Rural Settlement in the Brazilian Amazon

Journal of Medical Entomology ◽

10.1093/jme/tjab208 ◽

2022 ◽

Author(s):

R C S Guimarães ◽

E F Marialva ◽

J A Feijó ◽

J W Pereira-Silva ◽

K M Martins-Campos ◽

...

Keyword(s):

Natural Infection ◽

Species Abundance ◽

First Record ◽

Rural Settlement ◽

Sand Fly ◽

Sand Flies ◽

Forest Environment ◽

Genetic Sequencing ◽

Transmission Cycle ◽

Pcr Rflp

Abstract Trypanosomatids (Kinetoplastida:Trypanosomatidae) protozoa are a diverse group of obligate parasites. The genera Trypanosoma and Leishmania are the most studied because of their medical importance. This work aims to evaluate the effects of anthropization processes on the composition of the phlebotomine sand fly fauna and the natural infection by Trypanosomatids, with emphasis on Leishmania. At all 3,186 sand flies were collected, distributed in 13 genera and 52 species, being Ny. umbratilis the most abundant species. There was no difference in the diversity between canopy and soil environments. The species abundance and richness were higher in the forest environment while species diversity and evenness were highest in the forest edge. The ITS1 region was used by PCR-RFLP to identify the fragment profiles of Leishmania species, followed by genetic sequencing. Here were analyzed 100 pools of female sand flies, being six positive for DNA parasite. PCR-RFLP fragment patterns similar to Endotrypanum sp. were observed in Nyssomyia anduzei, Psychodopygus amazonensis and Lutzomyia gomezi, and those fragments similar to Leishmania (Leishmania) amazonensis were observed in Bichromomyia flaviscutellata. ITS1 sequencing confirmed the presence of Leishmania sp. in Bi. flaviscutellata, and Leishmania (Viannia) naiffi in Ny. anduzei, Psychodopygus amazonensis, and Lu. gomezi. This is the first record of Lu. gomezi and Ps. amazonensis infection by L. naiffi in the State of Amazonas. These results show the trypanosomatid infection in sandflies from different landscapes in a rural settlement, and the finding of species infected with L.(V.) naiffi suggest that they can develop a role in the transmission cycle of leishmaniasis.

Download Full-text

Identification of Turnip mosaic virus Isolates Causing Yellow Stripe and Spot on Calla Lily

Plant Disease ◽

10.1094/pdis.2003.87.8.901 ◽

2003 ◽

Vol 87 (8) ◽

pp. 901-905 ◽

Cited By ~ 37

Author(s):

C. C. Chen ◽

C. H. Chao ◽

C. C. Chen ◽

S. D. Yeh ◽

H. T. Tsai ◽

...

Keyword(s):

Mosaic Virus ◽

Natural Infection ◽

Sodium Dodecyl ◽

First Record ◽

Turnip Mosaic Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Calla Lily ◽

Black Magic ◽

Yellow Stripe ◽

Virus Isolates

Two virus cultures, RC4 and YC5, were isolated in Taiwan from calla lily (Zantedeschia spp.) cv. Black magic displaying yellow spot and stripe on leaves. Both isolates were mechanically transmitted to various hybrids of Zantedeschia and induced systemic symptoms similar to those observed on diseased Black magic. In addition to Zantedeschia spp., the two virus isolates also infected several cruciferous species and induced mosaic symptoms. Electron microscopy revealed the presence of flexuous virus particles about 750 nm in length. The two isolates were propagated in and purified from mustard plants and were used as immunogens for production of antisera in rabbits. In enzyme-linked immunosorbent assay and sodium dodecyl sulfate-immunodiffusion tests, both antisera reacted strongly with their homologous antigens and with antigens of two Turnip mosaic virus (TuMV) isolates from radish (TuMV-R) and lisianthus (TuMV-L), but not with 21 other different potyviruses tested. In reciprocal tests, antisera against TuMV-R and TuMV-L also reacted strongly with RC4 and YC5 antigens, indicating that these two calla lily isolates are serologically indistinguishable from other known TuMV strains. Cloning and sequence analyses confirmed that both isolates shared 95 to 99% of deduced amino acid sequence identities in the coat protein genes with those of various known TuMV strains. This investigation represents the first record of the natural infection of TuMV in calla lily.

Download Full-text

Natural infection of Oryctolagus cuniculus (Lagomorpha, Leporidae) by Gongylonema neoplasticum (Nematoda, Gongylonematidae) in Portugal

Acta Parasitologica ◽

10.2478/s11686-006-0018-4 ◽

2006 ◽

Vol 51 (2) ◽

Cited By ~ 5

Author(s):

Catarina Eira ◽

Jordi Miquel ◽

José Vingada ◽

Jordi Torres

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Geographical Distribution ◽

Oryctolagus Cuniculus ◽

Natural Infection ◽

First Record ◽

Surface Features ◽

Biological Features ◽

Host Spectrum ◽

Scanning Electron

AbstractGongylonema neoplasticum was identified in the oesophagus of 14 wild rabbits (Oryctolagus cuniculus) from Portugal. This is the first record of Gongylonema neoplasticum in a naturally infected lagomorph species in Europe. This paper presents the most relevant measurements of adult worms and some of their surface features seen by scanning electron microscopy. Epidemiological aspects of G. neoplasticum such as geographical distribution, host spectrum and biological features are discussed.

Download Full-text

First record of natural infection of chickpea byTurnip mosaic virus

Australasian Plant Disease Notes ◽

10.1071/dn07020 ◽

2007 ◽

Vol 2 (1) ◽

pp. 41 ◽

Cited By ~ 9

Author(s):

M. W. Schwinghamer ◽

J. E. Thomas ◽

J. N. Parry ◽

M. A. Schilg ◽

E. K. Dann

Keyword(s):

Mosaic Virus ◽

Natural Infection ◽

First Record

Download Full-text

Detection of Leishmania infantum DNA in Pintomyia evansi and Lutzomyia longipalpis in an endemic area of Non-ulcerative cutaneous leishmaniasis from Honduras

10.21203/rs.3.rs-36505/v1 ◽

2020 ◽

Author(s):

Wilfredo Humberto Sosa-Ochoa ◽

Javier Varela Amador ◽

Yokomi N Lozano-Sardaneta ◽

Gabriela Rodriguez Segura ◽

Concepcion Zúniga ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Leishmania Infantum ◽

Endemic Area ◽

Natural Infection ◽

First Record ◽

Abundant Species ◽

Lutzomyia Longipalpis ◽

Sand Flies ◽

Endemic Region ◽

Transmission Cycle

Abstract Background The two most abundant sand flies species in Honduran Pacific coast are Lutzomyia (Lutzomyia) longipalpis and Pintomyia (Pintomyia) evansi. Both species are proved vectors of the agent of for visceral leishmaniasis in South America. Although, visceral and cutaneous non-ulcerated leishmaniasis are endemic of the Central American Pacific, being the Non-ulcerative cutaneous leishmaniasis the most frequent manifestation. In this study, we evaluate the circulation of Leishmania spp in sand flies species in the El Tigre Island, an endemic area of Visceral and Non -ulcerative cutaneous leishmaniasis in Honduras. Results Six sand flies species were identified, being Lu. longipalpis (81%) and Pi. evansi (16%) the more abundant species. Leishmania (Leishmania) infantum DNA was found in 9 of the 96 specimens analyzed, 7 of these specimens was identify as Lutzomyia (Lutzomyia) longipalpis and 2 as Pintomyia (Pifanomyia) evansi, with an infection rate of 9.4% and 2.7% respectively. Conclusion Our results present the first record of Leishmania (L.) infantum DNA in Pintomyia (Pintomyia) evansi in a Non-ulcerative cutaneous leishmaniasis endemic region from Central America. Considering the natural infection of Lu. longipalpis our results suggest that Pintomyia (Pifanomyia) evansi might be a secondary vector of Leishmania (L.) infantum and probably involved in the disease’s transmission cycle. Undoubtedly, the detection of natural infections of in this region contributes to the understanding of the L. (L.) infantum infection epidemiology in Honduras

Download Full-text

Dracunculus insignis (Leidy, 1858) and larval Eustrongylides sp. in a muskrat from Ontario, Canada

Canadian Journal of Zoology ◽

10.1139/z72-120 ◽

1972 ◽

Vol 50 (6) ◽

pp. 897-901 ◽

Cited By ~ 2

Author(s):

G. G. Gibson ◽

D. A. McKiel

Keyword(s):

Natural Infection ◽

First Record

A muskrat, Ondatra zibethica, from northcentral Ontario harbored Dracunculus insignis (Leidy, 1858) Chandler, 1942 subcutaneously. This is the fifth report of Dracunculus sp. in muskrat and the first record of D. insignis males in this host; they are described briefly. The occurrence of Eustrongylides sp. larvae intramuscularly in this muskrat is the first reported natural infection of a mammal by nematodes of this genus.

Download Full-text

Role of hyperbenthic crustaceans in the transmission of marine helminth parasites

Canadian Journal of Fisheries and Aquatic Sciences ◽

10.1139/f96-329 ◽

1997 ◽

Vol 54 (4) ◽

pp. 815-820 ◽

Cited By ~ 15

Author(s):

C J Jackson ◽

D J Marcogliese ◽

M DB Burt

Keyword(s):

Natural Infection ◽

First Record ◽

Helminth Parasites ◽

Fish Parasite ◽

Infection Rates ◽

Nematode Parasites ◽

Helminth Infections ◽

Pseudoterranova Decipiens ◽

Hysterothylacium Aduncum

Invertebrates (n = 34<|>813), collected from nine areas in the Northwest Atlantic, were either dissected or enzymatically digested to determine if the animals harboured helminth infections. Six species of endoparasites were found. Mysids were host to the larval nematodes Pseudoterranova decipiens (sealworm), Hysterothylacium aduncum, and Paracuaria adunca, which are found as adults in seals, fish, and birds, respectively, and to the digenean fish parasite Hemiurus levinseni (first record in mysids). Larval Paracuaria adunca and Tetrameres sp., both nematode parasites of birds, and Ascarophis sp., a parasite of fish, were found in amphipods. Some Ascarophis sp. and H. levinsenirecovered from the crustaceans exhibited precocious sexual development. Natural infection rates of helminths in invertebrates are low. Mysids appear to be more important hosts than amphipods for transmission of sealworm to fish.

Download Full-text

First record of natural infection with Anaplasma marginale in sucking lice infesting the water buffalo (Bubalus bubalis) in Mexico

Parasitology Research ◽

10.1007/s00436-020-06772-7 ◽

2020 ◽

Vol 119 (11) ◽

pp. 3853-3856

Author(s):

Alejandra Hernández-Velasco ◽

Sokani Sánchez-Montes ◽

Dora Romero-Salas ◽

Anabel Cruz-Romero ◽

José Alfredo Jiménez-Hernández ◽

...

Keyword(s):

Water Buffalo ◽

Natural Infection ◽

Anaplasma Marginale ◽

First Record ◽

Bubalus Bubalis ◽

Sucking Lice

Download Full-text

Ultrastructural and molecular identification of Sarcocystis tenella (Protozoa, Apicomplexa) in naturally infected Korean native goats

Veterinární Medicína ◽

10.17221/93/2015-vetmed ◽

2016 ◽

Vol 61 (No. 7) ◽

pp. 374-381 ◽

Cited By ~ 2

Author(s):

EJ Hong ◽

C. Sim ◽

JS Chae ◽

HC Kim ◽

J. Park ◽

...

Keyword(s):

Intermediate Host ◽

Natural Infection ◽

Low Frequency ◽

Protozoan Parasite ◽

First Record ◽

Capra Hircus ◽

Histopathological Study ◽

Electron Microscopic ◽

The Republic ◽

Sarcocystis Tenella

Sheep is the intermediate host of the protozoan parasite Sarcocystis tenella, while the dog is its definitive host. This study was conducted to determine the prevalence of natural infection with S. tenella in slaughtered Korean native goat, Capra hircus coreanae, in the Republic of Korea. H-E stained heart tissues were investigated for the presence of sarcocysts. Of the 103 goats, three (2.91%) were diagnosed as positive for S. tenella by light, electron microscopic and molecular examination. The histopathological study showed a low frequency of microscopic Sarcocystis infection in slaughtered goats. In transmission electron microscopy, the sarcocysts were confirmed as S. tenella. Further DNA sequencing and phylogenic analysis support our identification of S. tenella with a 18S rRNA sequence identity of 100% between the experimental sequence and S. tenella. To our knowledge, this is the first record of S. tenella in Korean native goats from Korea. We thus report that the domestic goat is another intermediate host for S. tenella.

Download Full-text