Incongruence between morphological and molecular markers in the butterfly genusZizina(Lepidoptera: Lycaenidae) in New Zealand

2012 ◽  
Vol 38 (1) ◽  
pp. 151-163 ◽  
Author(s):  
MARK GILLESPIE ◽  
STEVE D. WRATTEN ◽  
ROB CRUICKSHANK ◽  
BENJAMIN H. WISEMAN ◽  
GEORGE W. GIBBS
Keyword(s):  
Molecular Markers ◽  
New Zealand

scholarly journals Association of polymorphisms in the GH and GHR genes with growth and carcass traits in rabbits (Oryctolagus cuniculus)

2019 ◽  
Vol 64 (No. 6) ◽  
pp. 255-264
Author(s):  
Lukasz Migdal ◽  
Sylwia Palka ◽  
Michal Kmiecik ◽  
Olga Derewicka
Keyword(s):  
Molecular Markers ◽  
New Zealand ◽  
Oryctolagus Cuniculus ◽  
Growth Traits ◽  
Carcass Traits ◽  
Breeding Programme ◽  
Association Analyses ◽  
Meat Weight ◽  
Dressing Percentage ◽  
Intermediate Part

In rabbits, growth and carcass traits are important for the breeding programme. An increasing number of annotated polymorphisms demands validation of their influence on those traits before they can be implemented in breeding practice. Therefore, the aim of this study was to investigate GH c.-78C>T, GHR c.106G>C polymorphisms in the population of Belgian Giant Grey, Termond White, and a crossbreed between New Zealand White and Belgian Giant Grey (NZW × BGG) rabbits. In total 379 animals were genotyped and association analyses with growth traits and carcass traits were conducted. Our results demonstrated that GH c.-78C>T showed an association with growth weight in Belgian Grey and NZW × BGG rabbits. Meat weight in intermediate and hind parts for GH c.-78C>T statistically differed between Belgian Giant Grey and crossbred rabbits. GHR c.106G>C showed an association with meat weight in the intermediate part and dressing percentage in Termond White. TT/CC haplotype in Belgian Giant Grey had significantly higher meat weight in hind part, while in crossbred rabbits CC/CC haplotype was characterised by the lowest meat weight in intermediate and hind parts. Results from our study confirm that GH c.-78C>T, GHR c.106G>C polymorphisms constitute good molecular markers for growth and carcass traits.

scholarly journals Molecular support for Pleistocene persistence of the continental Antarctic moss Bryum argenteum

2010 ◽  
Vol 22 (6) ◽  
pp. 721-726 ◽  
Author(s):  
Simon F.K. Hills ◽  
Mark I. Stevens ◽  
Chrissen E.C. Gemmill
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
New Zealand ◽  
Sequence Variation ◽  
Sister Group ◽  
Bryum Argenteum ◽  
Victoria Land ◽  
Dna Substitution ◽  
Geographic Regions ◽  
Antarctic Moss

AbstractWe examined sequence variation of ITS and phy2 for Bryum argenteum from Antarctica, sub-Antarctic, New Zealand and Australia to understand better taxonomic delimitations and resolve relationships between these geographic regions. Bryum argenteum has been recorded as two species, B. argenteum and B. subrotundifolium, in all four regions with the latter now referred to as B. argenteum var. muticum. We found disagreement between taxon delimitations (based on morphology) and molecular markers. All continental Antarctic specimens consistently formed a monophyletic sister group that consisted of both morphologically identified B. argenteum varieties, separate to all non-Antarctic specimens (also consisting of both varieties). We suggest, contrary to previous records, that all continental Antarctic (Victoria Land) populations are referable to B. argenteum var. muticum, while sub-Antarctic, Australian and New Zealand populations included here are B. argenteum var. argenteum. Additionally, since there was less genetic diversity within Victoria Land, Antarctica, than observed between non-Antarctic samples, we suggest that this is, in part, due to a potentially lower rate of DNA substitution and isolation in northern and southern refugia within Victoria Land since the Pleistocene.

scholarly journals Meiotic drive is associated with sexual incompatibility in Neurospora

2020 ◽  
Author(s):  
Aaron Vogan ◽  
Jesper Svedberg ◽  
Magdalena Grudzinska-Sterno ◽  
Hanna Johannesson
Keyword(s):  
Molecular Markers ◽  
New Zealand ◽  
Reproductive Isolation ◽  
Evolutionary History ◽  
Meiotic Drive ◽  
Sister Species ◽  
Independent Evolutionary History

AbstractMeiotic drive is the phenomenon whereby selfish elements bias their transmission to progeny at ratios above 50:50, violating Mendel’s law of equal segregation. The model fungus Neurospora carries three different meiotic drivers, called Spore killers. Two of these, Sk-2 and Sk-3, are multilocus spore killers that constitute large haplotypes and are found in the species N. intermedia. Here we used molecular markers to determine that all N. intermedia isolates from New Zealand in fact belong to the sister species N. metzenbergii. Additionally, we use laboratory crosses to demonstrate that Sk-2 and Sk-3 are involved in sexual incompatibility between N. intermedia and N. metzenbergii.. Our experiments revealed that while crosses between these two species normally produced viable progeny at appreciable rates, when strains of N. intermedia carried Sk-2 or Sk-3 the proportion of viable progeny dropped substantially and in some crosses, no viable progeny were observed. Backcrossings supported that the incompatibility is tightly linked to the Sk haplotype. Finally, it appears that Sk-2 and Sk-3 have accumulated different incompatibility phenotypes when crossed with N. metzenbergii strains, consistent with their independent evolutionary history. This research illustrates how meiotic drive can contribute to reproductive isolation between populations, and thereby speciation.

scholarly journals Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand

2018 ◽  
Vol 6 (9) ◽  
pp. e1181
Author(s):  
Claudia Lange ◽  
Dagmar F. Goeke ◽  
Graeme W. Bourdôt ◽  
Shona L. Lamoureaux ◽  
Ana Podolyan ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Molecular Markers ◽  
New Zealand

Magellanic Cloud Eclipsing Binaries: Primary Distance Indicators

1999 ◽  
Vol 190 ◽  
pp. 563-566
Author(s):  
J. D. Pritchard ◽  
W. Tobin ◽  
J. V. Clausen ◽  
E. F. Guinan ◽  
E. L. Fitzpatrick ◽  
...  
Keyword(s):  
New Zealand ◽  
Magellanic Cloud ◽  
Eclipsing Binaries ◽  
Fundamental Parameters ◽  
Distance Indicators

Our collaboration involves groups in Denmark, the U.S.A. Spain and of course New Zealand. Combining ground-based and satellite (IUEandHST) observations we aim to determine accurate and precise stellar fundamental parameters for the components of Magellanic Cloud Eclipsing Binaries as well as the distances to these systems and hence the parent galaxies themselves. This poster presents our latest progress.

Heat-Etching with a Bullivant Type II Simple Freeze-Cleave Device

1970 ◽  
Vol 28 ◽  
pp. 106-107 ◽  
Author(s):  
Ronald S. Weinstein ◽  
N. Scott McNutt
Keyword(s):  
New Zealand ◽  
General Hospital ◽  
Massachusetts General Hospital ◽  
Type I ◽  
Type Ii ◽  
Collaborative Effort ◽  
Temperature And Pressure ◽  
The University

The Type I simple cold block device was described by Bullivant and Ames in 1966 and represented the product of the first successful effort to simplify the equipment required to do sophisticated freeze-cleave techniques. Bullivant, Weinstein and Someda described the Type II device which is a modification of the Type I device and was developed as a collaborative effort at the Massachusetts General Hospital and the University of Auckland, New Zealand. The modifications reduced specimen contamination and provided controlled specimen warming for heat-etching of fracture faces. We have now tested the Mass. General Hospital version of the Type II device (called the “Type II-MGH device”) on a wide variety of biological specimens and have established temperature and pressure curves for routine heat-etching with the device.

A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

1975 ◽  
Vol 33 ◽  
pp. 460-461
Author(s):  
Sidney D. Kobernick ◽  
Edna A. Elfont ◽  
Neddra L. Brooks
Keyword(s):  
Lipid Metabolism ◽  
New Zealand ◽  
Aortic Wall ◽  
Rabbit Aorta ◽  
Plaque Formation ◽  
Metabolic Changes ◽  
Atherosclerotic Plaques ◽  
Cytochemical Study ◽  
Cellular Alteration ◽  
New Zealand White Rabbits

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

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