Arabidopsis thaliana plastidial methionine sulfoxide reductases B, MSRBs, account for most leaf peptide MSR activity and are essential for growth under environmental constraints through a role in the preservation of photosystem antennae

Norway maple and sycamore produce desiccation-tolerant (orthodox) and desiccation-sensitive (recalcitrant) seeds, respectively. Drying affects reduction and oxidation (redox) status in seeds. Oxidation of methionine to methionine sulfoxide (MetO) and reduction via methionine sulfoxide reductases (Msrs) have never been investigated in relation to seed desiccation tolerance. MetO levels and the abundance of Msrs were investigated in relation to levels of reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion radical and hydroxyl radical (•OH), and the levels of ascorbate and glutathione redox couples in gradually dried seeds. Peptide-bound MetO levels were positively correlated with ROS concentrations in the orthodox seeds. In particular, •OH affected MetO levels as well as the abundance of MsrB2 solely in the embryonic axes of Norway maple seeds. In this species, MsrB2 was present in oxidized and reduced forms, and the latter was favored by reduced glutathione and ascorbic acid. In contrast, sycamore seeds accumulated higher ROS levels. Additionally, MsrB2 was oxidized in sycamore throughout dehydration. In this context, the three elements •OH level, MetO content and MsrB2 abundance, linked together uniquely to Norway maple seeds, might be considered important players of the redox network associated with desiccation tolerance.

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Functional characterization of methionine sulfoxide reductases from Leptospira interrogans

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2020.140575 ◽

2021 ◽

Vol 1869 (2) ◽

pp. 140575

Author(s):

Natalia Sasoni ◽

Matías D. Hartman ◽

Sergio A. Guerrero ◽

Alberto A. Iglesias ◽

Diego G. Arias

Keyword(s):

Functional Characterization ◽

Methionine Sulfoxide ◽

Leptospira Interrogans ◽

Methionine Sulfoxide Reductases

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Thioredoxins and Methionine Sulfoxide Reductases in the Pathophysiology of Pneumococcal Meningitis

The Journal of Infectious Diseases ◽

10.1093/infdis/jiw268 ◽

2016 ◽

Vol 214 (6) ◽

pp. 953-961 ◽

Cited By ~ 8

Author(s):

Sandra Ribes ◽

Mohammed R. Abdullah ◽

Malek Saleh ◽

Uwe-Karsten Hanisch ◽

Roland Nau ◽

...

Keyword(s):

Pneumococcal Meningitis ◽

Methionine Sulfoxide ◽

Methionine Sulfoxide Reductases

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Involvement of the MetO/Msr System in Two Acer Species That Display Contrasting Characteristics during Germination

International Journal of Molecular Sciences ◽

10.3390/ijms21239197 ◽

2020 ◽

Vol 21 (23) ◽

pp. 9197

Author(s):

Natalia Wojciechowska ◽

Shirin Alipour ◽

Ewelina Stolarska ◽

Karolina Bilska ◽

Pascal Rey ◽

...

Keyword(s):

Hydroxyl Radicals ◽

Superoxide Anion ◽

Redox Homeostasis ◽

Methionine Sulfoxide ◽

Redox System ◽

Oxygen Species ◽

Embryonic Axes ◽

Methionine Sulfoxide Reductases ◽

Germination Process ◽

Norway Maple

The levels of methionine sulfoxide (MetO) and the abundances of methionine sulfoxide reductases (Msrs) were reported as important for the desiccation tolerance of Acer seeds. To determine whether the MetO/Msrs system is related to reactive oxygen species (ROS) and involved in the regulation of germination in orthodox and recalcitrant seeds, Norway maple and sycamore were investigated. Changes in water content, MetO content, the abundance of MsrB1 and MsrB2 in relation to ROS content and the activity of reductases depending on nicotinamide adenine dinucleotides were monitored. Acer seeds differed in germination speed—substantially higher in sycamore—hydration dynamics, levels of hydrogen peroxide, superoxide anion radicals (O2•−) and hydroxyl radicals (•OH), which exhibited peaks at different stages of germination. The MetO level dynamically changed, particularly in sycamore embryonic axes, where it was positively correlated with the levels of O2•− and the abundance of MsrB1 and negatively with the levels of •OH and the abundance of MsrB2. The MsrB2 abundance increased upon sycamore germination; in contrast, it markedly decreased in Norway maple. We propose that the ROS–MetO–Msr redox system, allowing balanced Met redox homeostasis, participates in the germination process in sycamore, which is characterized by a much higher speed compared to Norway maple.

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Arabidopsis thaliana methionine sulfoxide reductase B8 influences stress-induced cell death and effector-triggered immunity

Plant Molecular Biology ◽

10.1007/s11103-016-0550-z ◽

2016 ◽

Vol 93 (1-2) ◽

pp. 109-120 ◽

Cited By ~ 18

Author(s):

Shweta Roy ◽

Ashis Kumar Nandi

Keyword(s):

Arabidopsis Thaliana ◽

Cell Death ◽

Methionine Sulfoxide ◽

Methionine Sulfoxide Reductase ◽

Effector Triggered Immunity

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Modulation of gene expressions and enzyme activities of methionine sulfoxide reductases by cold, ABA or high salt treatments in Arabidopsis

Plant Science ◽

10.1016/j.plantsci.2005.05.033 ◽

2005 ◽

Vol 169 (6) ◽

pp. 1030-1036 ◽

Cited By ~ 22

Author(s):

Jee-Eun Oh ◽

Suk-Whan Hong ◽

Yunsook Lee ◽

Eun-Ji Koh ◽

Kideok Kim ◽

...

Keyword(s):

Enzyme Activities ◽

Methionine Sulfoxide ◽

High Salt ◽

Gene Expressions ◽

Methionine Sulfoxide Reductases

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Expression of Four Methionine Sulfoxide Reductases inStaphylococcus aureus

International Journal of Microbiology ◽

10.1155/2012/719594 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 11

Author(s):

Kuldeep Singh ◽

Vineet K. Singh

Keyword(s):

Staphylococcus Aureus ◽

Stationary Phase ◽

Expression Pattern ◽

Growth Phase ◽

Methionine Sulfoxide ◽

Phase Growth ◽

Methionine Sulfoxide Reductases ◽

Reporter Strains

Staphylococcus aureuspossesses three MsrA enzymes (MsrA1, MsrA2, MsrA3) that reduce the S-epimer of methionine sulfoxide (MetO) and an MsrB enzyme that reduces R-MetO. The fourmsrgenes are expressed from three different promoters. ThemsrA1/msrBgenes are coexpressed. To determine the expression pattern ofmsrgenes, three independent reporter strains were constructed wheremsrpromoter was cloned in front of a promoterlesslacZand the resulting construct was integrated in the chromosome. Using these strains, it was determined that themsrA1/Bexpression is significantly higher inS. aureuscompared tomsrA2ormsrA3. Expression ofmsrA1/Bwas highest during stationary phase growth, but the expression ofmsrA2andmsrA3was highest during the early to midexponential growth phase. Expression ofmsrA1/Bwas induced by oxacillin and the expression ofmsrA3was upregulated by salt. Expression ofmsrA2remained unchanged under all tested conditions.

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