scholarly journals GIGANTEA is a component of a regulatory pathway determining wall ingrowth deposition in phloem parenchyma transfer cells of Arabidopsis thaliana

2010 ◽  
Vol 63 (4) ◽  
pp. 651-661 ◽  
Author(s):  
Joshua Edwards ◽  
Antony P. Martin ◽  
Felicity Andriunas ◽  
Christina E. Offler ◽  
John W. Patrick ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transfer Cells ◽  
Regulatory Pathway ◽  
Phloem Parenchyma ◽  
Wall Ingrowth

scholarly journals Unraveling the puzzle of phloem parenchyma transfer cell wall ingrowth

2020 ◽  
Vol 71 (16) ◽  
pp. 4617-4620 ◽  
Author(s):  
Tyler J McCubbin ◽  
David M Braun
Keyword(s):  
Cell Wall ◽  
Phloem Loading ◽  
Transfer Cells ◽  
Transfer Cell ◽  
Phloem Parenchyma ◽  
Wall Ingrowth ◽  
Experimental Botany

This article comments on: Wei X, Nguyen ST, Collings DA, McCurdy DW. 2020. Sucrose regulates wall ingrowth deposition in phloem parenchyma transfer cells in Arabidopsis via affecting phloem loading activity. Journal of Experimental Botany 71, 4690–4702.

scholarly journals Sucrose regulates wall ingrowth deposition in phloem parenchyma transfer cells in Arabidopsis via affecting phloem loading activity

2020 ◽  
Vol 71 (16) ◽  
pp. 4690-4702 ◽  
Author(s):  
Xiaoyang Wei ◽  
Suong T T Nguyen ◽  
David A Collings ◽  
David W McCurdy
Keyword(s):  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Phloem Loading ◽  
Transfer Cells ◽  
Null Mutant ◽  
Dark Treatment ◽  
Phloem Parenchyma ◽  
Wall Ingrowth ◽  
Overexpression Line ◽  
Regulated Expression

Abstract In Arabidopsis thaliana, phloem parenchyma transfer cells (PPTCs) occur in leaf minor veins and play a pivotal role in phloem loading. Wall ingrowth formation in PPTCs is induced by the phloem loading activity of these cells, which is regulated by sucrose (Suc). The effects of endogenous versus exogenous Suc on wall ingrowth deposition, however, differ. Elevating endogenous Suc levels by increased light enhanced wall ingrowth formation, whereas lowering endogenous Suc levels by dark treatment or genetically in ch-1 resulted in lower levels of deposition. In contrast, exogenously applied Suc, or Suc derived from other organs, repressed wall ingrowth deposition. Analysis of pAtSUC2::GFP plants, used as a marker for phloem loading status, suggested that wall ingrowth formation is correlated with phloem loading activity. Gene expression analysis revealed that exogenous Suc down-regulated expression of AtSWEET11 and 12, whereas endogenous Suc up-regulated AtSWEET11 expression. Analysis of a TREHALOSE 6-PHOSPHATE (T6P) SYNTHASE overexpression line and the hexokinase (HXK)-null mutant, gin2-1, suggested that Suc signalling of wall ingrowth formation is independent of T6P and HXK. Collectively, these results are consistent with the conclusion that Suc regulates wall ingrowth formation via affecting Suc exporting activity in PPTCs.

scholarly journals Asymmetric wall ingrowth deposition in Arabidopsis phloem parenchyma transfer cells is tightly associated with sieve elements

2022 ◽  
Author(s):  
Xiaoyang Wei ◽  
Yuan Huang ◽  
David A Collings ◽  
David W McCurdy
Keyword(s):  
Cell Types ◽  
Phloem Loading ◽  
Transfer Cells ◽  
Companion Cell ◽  
Wall Ingrowths ◽  
Phloem Parenchyma ◽  
Wall Ingrowth ◽  
Functional Differences ◽  
Transgenic Lines ◽  
Different Cell Types

In Arabidopsis, polarized deposition of wall ingrowths in phloem parenchyma (PP) transfer cells (TCs) occurs adjacent to cells of the sieve element/companion cell (SE/CC) complex. However, the spatial relationships between these different cell types in minor veins, where phloem loading occurs, are poorly understood. PP TC development and wall ingrowth localization were compared to other phloem cells in leaves of Col-0 and the transgenic lines AtSUC2::AtSTP9-GFP and AtSWEET11::AtSWEET11-GFP that identify CCs and PP respectively. The development of PP TCs in minor veins, indicated by deposition of wall ingrowths, proceeded basipetally in leaves. However, not all PP develop ingrowths and higher levels of wall ingrowth deposition occur in abaxial- compared to adaxial-positioned PP TCs. Furthermore, the deposition of wall ingrowths was exclusively initiated on and preferentially covered the PP TC/SE interface, rather than the PP TC/CC interface, and only occurred in PP cells that were adjacent to SEs. Collectively, these results demonstrate the dominant impact of SEs on wall ingrowth deposition in PP TCs and suggest the existence of two sub-types of PP cells in leaf minor veins. Compared to PP cells, PP TCs showed more abundant accumulation of AtSWEET11-GFP, indicating functional differences in phloem loading between PP and PP TCs.

Wall ingrowth architecture in epidermal transfer cells ofVicia faba cotyledons

PROTOPLASMA ◽  
2001 ◽  
Vol 215 (1-4) ◽  
pp. 191-203 ◽  
Author(s):  
Mark J. Talbot ◽  
Vincent R. Franceschi ◽  
David W. McCurdy ◽  
Christina E. Offler
Keyword(s):  
Transfer Cells ◽  
Wall Ingrowth ◽  
Ofvicia Faba

NaCl effect on the distribution of wall ingrowth polymers and arabinogalactan proteins in type A transfer cells of Medicago sativa Gabès leaves

PROTOPLASMA ◽  
2010 ◽  
Vol 242 (1-4) ◽  
pp. 69-80 ◽  
Author(s):  
Néziha Boughanmi ◽  
Florence Thibault ◽  
Raphael Decou ◽  
Pierrette Fleurat-Lessard ◽  
Emile Béré ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Type A ◽  
Arabinogalactan Proteins ◽  
Transfer Cells ◽  
Wall Ingrowth

scholarly journals Initial embedding of TRANSPARENT TESTA GLABRA 1 in the Arabidopsis thaliana flowering time regulatory pathway

2019 ◽  
Author(s):  
Barbara A M Paffendorf ◽  
Rawan Qassrawi ◽  
Andrea M Meys ◽  
Laura Trimborn ◽  
Andrea Schrader
Keyword(s):  
Arabidopsis Thaliana ◽  
Circadian Clock ◽  
Flowering Time ◽  
Response Regulator ◽  
Regulatory Pathway ◽  
In Planta ◽  
Transcript Levels ◽  
Transparent Testa ◽  
Flowering Locus

Pleiotropic regulatory factors mediate concerted responses of the plant’s trait network to endogenous and exogenous cues. TRANSPARENT TESTA GLABRA 1 (TTG1) is a pleiotropic regulator that has been predominantly described in its role as a regulator of early accessible developmental traits. Although its closest homologs LIGHT-REGULATED WD1 (LWD1) and LWD2 are regulators of photoperiodic flowering, a role of TTG1 in flowering time regulation has not been reported. Here we reveal that TTG1 is a regulator of flowering time in Arabidopsis thaliana and changes transcription levels of different targets within the flowering time regulatory pathway. TTG1 mutants flower early and TTG1 overexpression lines flower late at long-day conditions. Consistently, TTG1 can suppress the transcript levels of the floral integrators FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CO1 and can act as an activator of circadian clock components. Moreover, TTG1 might form feedback loops at the protein level. The TTG1 protein interacts with PSEUDO RESPONSE REGULATOR (PRR)s and basic HELIX-LOOP-HELIX 92 (bHLH92) in yeast. In planta, the respective pairs exhibit interesting patterns of localization including a recruitment of TTG1 by PRR5 to subnuclear foci. This mechanism proposes additional layers of regulation by TTG1 and might aid to specify the function of bHLH92. Within another branch of the pathway, TTG1 can elevate FLOWERING LOCUS C (FLC) transcript levels. FLC mediates signals from the vernalization, ambient temperature and autonomous pathway and the circadian clock is pivotal for the plant to synchronize with diurnal cycles of environmental stimuli like light and temperature. Our results suggest an unexpected positioning of TTG1 upstream of FLC and upstream of the circadian clock. In this light, this points to an adaptive value of the role of TTG1 in respect to flowering time regulation.

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