Immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b in early-onset periodontitis patients

1992 ◽  
Vol 7 (2) ◽  
pp. 65-70 ◽  
Author(s):  
J. V. Califano ◽  
H. A. Schenkein ◽  
J. G. Tew
Keyword(s):  
Early Onset ◽  
Actinobacillus Actinomycetemcomitans ◽  
Immunodominant Antigens ◽  
Serotype B

scholarly journals Influence of anti-Actinobacillus actinomycetemcomitans Y4 (serotype b) lipopolysaccharide on severity of generalized early-onset periodontitis.

1996 ◽  
Vol 64 (9) ◽  
pp. 3908-3910 ◽  
Author(s):  
J V Califano ◽  
J C Gunsolley ◽  
K Nakashima ◽  
H A Schenkein ◽  
M E Wilson ◽  
...  
Keyword(s):  
Early Onset ◽  
Actinobacillus Actinomycetemcomitans ◽  
Serotype B

Antibody reactive with Actinobacillus actinomycetemcomitans leukotoxin in early-onset periodontitis patients

1997 ◽  
Vol 12 (1) ◽  
pp. 20-26 ◽  
Author(s):  
J. V. Califano ◽  
B. E. Pace ◽  
J. C. Gunsolley ◽  
H. A. Schenkein ◽  
E. T. Lally ◽  
...  
Keyword(s):  
Early Onset ◽  
Actinobacillus Actinomycetemcomitans

scholarly journals Construction of mutants of Actinobacillus actinomycetemcomitans defective in serotype b-specific polysaccharide antigen by insertion of transposon Tn916

1992 ◽  
Vol 138 (9) ◽  
pp. 1992-1992
Author(s):  
S. Sato ◽  
N. Takamatsu ◽  
N. Okahashi ◽  
N. Matsunoshita ◽  
M. Inoue ◽  
...  
Keyword(s):  
Actinobacillus Actinomycetemcomitans ◽  
Polysaccharide Antigen ◽  
Specific Polysaccharide ◽  
Serotype B

scholarly journals Cloning of the Gene Encoding the Actinobacillus actinomycetemcomitans Serotype b OmpA-Like Outer Membrane Protein

1999 ◽  
Vol 67 (2) ◽  
pp. 942-945 ◽  
Author(s):  
Hitoshi Komatsuzawa ◽  
Toshihisa Kawai ◽  
Mark E. Wilson ◽  
Martin A. Taubman ◽  
Motoyuki Sugai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Membrane Protein ◽  
Amino Acid Sequence ◽  
Molecular Mass ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Actinobacillus Actinomycetemcomitans ◽  
Gene Encoding ◽  
Serotype B

ABSTRACT The gene encoding an outer membrane protein A (OmpA)-like, heat-modifiable Omp of Actinobacillus actinomycetemcomitans ATCC 43718 (strain Y4, serotype b) was cloned by a PCR cloning procedure. DNA sequence analysis revealed that the gene encodes a protein of 346 amino acid residues with a molecular mass of 36.9 kDa. The protein expressed by the cloned gene reacted with a monoclonal antibody to the previously described 29-kDa Omp (Omp29) of strain Y4. This monoclonal antibody reacted specifically with Omp29 of A. actinomycetemcomitans (serotype b), but not with any Omp of Escherichia coli, including OmpA. This protein exhibited characteristic heat modifiability on sodium dodecyl sulfate-polyacrylamide gels, showing an apparent molecular mass of 29 kDa when unheated and a mass of 34 kDa when heated. The N-terminal amino acid sequence of the protein expressed inE. coli perfectly matched those deduced from the purified Omp29 of strain Y4. The deduced amino acid sequence of the gene coding for Omp29 from serotype b matched completely (except for valine at position 321) that of a recently reported omp34gene described for A. actinomycetemcomitans serotype c (NCTC 9710). Because of the conserved nature of the gene within these serotypes, we designated the gene described herein from serotype b asomp34.

scholarly journals Structural and Genetic Analyses of O Polysaccharide from Actinobacillus actinomycetemcomitans Serotype f

2001 ◽  
Vol 69 (9) ◽  
pp. 5375-5384 ◽  
Author(s):  
Jeffrey B. Kaplan ◽  
Malcolm B. Perry ◽  
Leann L. MacLean ◽  
David Furgang ◽  
Mark E. Wilson ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Gene Clusters ◽  
Pcr Primers ◽  
Cross Reactivity ◽  
Actinobacillus Actinomycetemcomitans ◽  
Molar Ratio ◽  
African American Female ◽  
Specific Pcr ◽  
Serotype B ◽  
Transposon Insertions

ABSTRACT The oral bacterium Actinobacillus actinomycetemcomitans is implicated as a causative agent of localized juvenile periodontitis (LJP). A. actinomycetemcomitans is classified into five serotypes (a to e) corresponding to five structurally and antigenically distinct O polysaccharide (O-PS) components of their respective lipopolysaccharide molecules. Serotype b has been reported to be the dominant serotype isolated from LJP patients. We determined the lipopolysaccharide O-PS structure from A. actinomycetemcomitans CU1000, a strain isolated from a 13-year-old African-American female with LJP which had previously been classified as serotype b. The O-PS of strain CU1000 consisted of a trisaccharide repeating unit composed ofl-rhamnose and 2-acetamido-2-deoxy-d-galactose (molar ratio, 2:1) with the structure →2)-α-l-Rhap-(1–3)-2-O-(β-d-GalpNAc)-α-l-Rhap-(1→. O-PS from strain CU1000 was structurally and antigenically distinct from the O-PS molecules of the five known A. actinomycetemcomitans serotypes. Strain CU1000 was mutagenized with transposon IS903φkan, and three mutants that were deficient in O-PS synthesis were isolated. All three transposon insertions mapped to a single 1-kb region on the chromosome. The DNA sequence of a 13.1-kb region surrounding these transposon insertions contained a cluster of 14 open reading frames that was homologous to gene clusters responsible for the synthesis of A. actinomycetemcomitans serotype b, c, and e O-PS antigens. The CU1000 gene cluster contained two genes that were not present in serotype-specific O-PS antigen clusters of the other five knownA. actinomycetemcomitans serotypes. These data indicate that strain CU1000 should be assigned to a new A. actinomycetemcomitans serotype, designated serotype f. A PCR assay using serotype-specific PCR primers showed that 3 out of 20 LJP patients surveyed (15%) harbored A. actinomycetemcomitans strains carrying the serotype f gene cluster. The finding of an A. actinomycetemcomitansserotype showing serological cross-reactivity with anti-serotype b-specific antiserum suggests that a reevaluation of strains previously classified as serotype b may be warranted.

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