Effects of ovarian fluid on motility characteristics of rainbow trout (Oncorhynchus mykissWalbaum) spermatozoa

2008 ◽  
Vol 24 (4) ◽  
pp. 503-507 ◽  
Author(s):  
G. J. Dietrich ◽  
M. Wojtczak ◽  
M. Słowińska ◽  
S. Dobosz ◽  
H. Kuźmiński ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Ovarian Fluid

scholarly journals Post-ovulatory secretion of pituitary gonadotropins GtH I and GtH II in the rainbow trout (Oncorhynchus mykiss): regulation by steroids and possible role of non-steroidal gonadal factors

1999 ◽  
Vol 163 (1) ◽  
pp. 87-97 ◽  
Author(s):  
J Chyb ◽  
T Mikolajczyk ◽  
B Breton
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Body Cavity ◽  
The Body ◽  
Ovarian Fluid ◽  
Multiple Injections ◽  
Rainbow Trout Oncorhynchus Mykiss

In order to determine the factors of ovarian origin which can modulate the postovulatory secretion of the FSH-like gonadotropin (GtH I) and the LH-like gonadotropin (GtH II), freshly ovulated female rainbow trout were divided into two groups. In the first group the fish were stripped in order to eliminate the eggs and ovarian fluid from the body cavity, while in the second group the eggs were kept in the body cavity. Subsequently, fish from both groups were implanted with testosterone (10 mg/kg), 17beta-estradiol (10 mg/kg) or 17,20beta-ddihydroxy-4-regnen-3-one (17,20betaP) (1 mg/kg) or injected every 2 days with desteroidized ovarian fluid (1.5 ml/kg). The secretion of GtH I dramatically increased in stripped fish, reaching its maximum levels 2 weeks after ovulation. The preservation of eggs in the body cavity led to the suppression of this increase. The profiles of GtH II secretion were opposite to those encountered for GtH I because the increase of GtH II was observed only in unstripped fish. The administration of steroids showed that testosterone is able to inhibit GtH I release and stimulate that of GtH II in stripped fish, having no effect on the release of these gonadotropins in non-stripped animals. 17beta-Estradiol failed to modify GtH I secretion, however it decreased the release of GtH II in fish containing retained eggs in the body cavity. 17,20betaP had a delayed stimulating influence on GtH I release in unstripped fish. Finally, multiple injections of desteroidized ovarian fluid into stripped fish led to a significant decrease of GtH I release and to an increase of GtH II secretion. This study demonstrates that factors, which are present in ovarian fluid, modulate the post-ovulatory secretion of both gonadotropins--their net action is negative on GtH I and positive on GtH II. Among the steroids, testosterone is of major importance, being able to inhibit GtH I release and to stimulate that of GtH II. We also show that non-steroidal factors present in the ovarian fluid can influence the release of both gonadotropins, which indirectly supports the previous findings about the existence of inhibin/activin-like factors in fish.

scholarly journals Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

2021 ◽  
Vol 50 (2) ◽  
pp. 160-168
Author(s):  
Marcin Polonis ◽  
Agata Błaszczyk ◽  
Krzysztof Jagiełło ◽  
Ligia Panasiak ◽  
Stefan Dobosz ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Rainbow Trout ◽  
Ph Value ◽  
Survival Rates ◽  
Nuclear Genome ◽  
Developmental Competence ◽  
X Rays ◽  
Ovarian Fluid ◽  
Developmental Potential ◽  
Rainbow Trout Oncorhynchus Mykiss

Abstract Ionizing radiation (IR) is applied to inactivate the nuclear genome in rainbow trout eggs during induced androgenetic development. However, IR-generated reactive oxygen species (ROS) may affect developmental potential of eggs and reduce the effectiveness of androgenesis. To verify this assumption, androgenetic development of rainbow trout was induced in eggs irradiated with 350 Gy of X-rays. Survival rates, pH of the ovarian fluid and activity of antioxidant enzymes, including SOD, CAT and GPx, were examined in non-irradiated and irradiated eggs originating from four females. Survival rates of androgenetic embryos developing in eggs produced by different females varied from 1% to 57% and these inter-clutch differences were significant. Eggs from female F4, which showed the highest developmental competence for androgenesis, also showed increased activities of SOD, CAT and GPx enzymes. The pH value of the ovarian fluid of each female was over 8 before and after irradiation, therefore it seems that radiation did not affect the ovarian fluid pH. Considering the above-mentioned inter-clutch differences, a strong maternal effect on the effectiveness of androgenesis can be assumed. Eggs with increased activity of antioxidant enzymes before irradiation should be expected to show increased developmental competence for androgenesis.

Shotgun proteomics of rainbow trout ovarian fluid

2015 ◽  
Vol 27 (3) ◽  
pp. 504 ◽  
Author(s):  
Joanna Nynca ◽  
Georg J. Arnold ◽  
Thomas Fröhlich ◽  
Andrzej Ciereszko
Keyword(s):  
Rainbow Trout ◽  
Cell Structure ◽  
Cell Lineage ◽  
Myeloid Cell ◽  
Shotgun Proteomics ◽  
Oocyte Quality ◽  
Lipid Binding ◽  
Ovarian Fluid ◽  
Proteomic Approach ◽  
Vitelline Envelope

In the present study we used a shotgun proteomic approach to identify 54 proteins of rainbow trout ovarian fluid. The study has unravelled the identity of several proteins not previously reported in fish ovarian fluid. The proteome of trout ovarian fluid consists of diverse proteins participating in lipid binding and metabolism, carbohydrate and ion transport, innate immunity, maturation and ovulation processes. Most trout ovarian fluid proteins correspond to follicular fluid proteins of higher vertebrates, but 15% of the proteins were found to be different, such as those related to the immune system (precerebellin-like protein), proteolysis (myeloid cell lineage chitinase), carbohydrate and lipid binding and metabolism (vitellogenins), cell structure and shape (vitelline envelope protein gamma) and a protein with unknown functions (UPF0762 protein C6orf58 homologue). The present study could help in the decoding of the biological function of these proteins and in the discovery of potential biomarkers of oocyte quality.

Structure of novel gangliosides, deaminated neuraminic acid (KDN) containing glycosphingolipids, isolated from rainbow trout ovarian fluid

Biochemistry ◽  
1993 ◽  
Vol 32 (35) ◽  
pp. 9221-9229 ◽  
Author(s):  
Yu Song ◽  
Ken Kitajima ◽  
Sadako Inoue ◽  
Yutaka Muto ◽  
Takeshi Kasama ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Neuraminic Acid ◽  
Ovarian Fluid

Modification of the Rheotropic Behavior of Male Rainbow Trout (Salmo gairdneri) by Ovarian Fluid

1979 ◽  
Vol 36 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Mary E. Emanuel ◽  
Julian J. Dodson
Keyword(s):  
Rainbow Trout ◽  
Key Words ◽  
Salmo Gairdneri ◽  
Fish Migration ◽  
Odor Plume ◽  
Ovarian Fluid ◽  
Water Currents ◽  
Olfactory Stimuli

The upstream orientation and frequency of upstream movement of ripe male rainbow trout (Salmo gairdneri) in the presence of ovarian fluid obtained from spawning females of the same species were observed and quantified. In a stream tank, male trout moved upstream in the ovarian fluid odor plume. In an optomotor tank, male trout exhibited an increase in the frequency of upstream orientation and movement in the presence of diffuse, nondirectional ovarian fluid. It is concluded that water currents provide directional cues and olfactory stimuli regulate the orientational and kinetic components of the rheotropic response. The significance of this mechanism of orientation in fish migration is discussed. Key words: rheotropism, behavior, olfaction, orientation, migration, ovarian fluid

scholarly journals Does the rainbow trout ovarian fluid guide the spermatozoon on its way to the egg?

2021 ◽  
Author(s):  
Vitaliy Kholodnyy ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Hermes Bloomfield-Gadelha ◽  
Manabu Yoshida ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Freshwater Fish ◽  
High Velocity ◽  
Chemotactic Response ◽  
Ovarian Fluid ◽  
Motility Pattern ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Male Gametes ◽  
The Individual

Fertilization of freshwater fish occurs in an environment that may affect negatively the gametes, therefore the specific mechanisms triggering the encounters of gametes would be highly expedient. The egg and ovarian fluid (OF) are likely the major sources of these triggers in fish, that we confirmed here for rainbow trout Oncorhynchus mykiss. The ovarian fluid affected significantly the spermatozoa performance: it supported high velocity for a longer period and changed the motility pattern from tumbling in water to straightforward moving in the ovarian fluid. Rainbow trout OF induced a trapping chemotaxis-like effect on activated male gametes and this effect depended on the properties of the activating media. The interaction of the sper-matozoa with the attracting agents was accompanied by their 'turn-and-run' behavior involving asymmetric flagellar beating and Ca2+ concentration bursts in the bent flagella segment, characteristic for the chemotactic response. Collectively, the ovarian fluid creates the optimal environment for rainbow trout spermatozoa performance, being an effective promoter of fertilization. The individual peculiarities of the egg (ovarian fluid)-sperm interaction in rainbow trout reflect the specific features of the spawning process in this species.

Composition of Ovarian Fluid and Eggs of the University of Washington Strain of Rainbow Trout (Salmo gairdneri)

1974 ◽  
Vol 31 (11) ◽  
pp. 1796-1799 ◽  
Author(s):  
Benedict P. Satia ◽  
Lauren R. Donaldson ◽  
Lynwood S. Smith ◽  
John N. Nightingale
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Total Bilirubin ◽  
Essential Amino Acids ◽  
Salmo Gairdneri ◽  
Ovarian Fluid ◽  
University Of Washington ◽  
The University

The concentrations of major constituents of ovarian fluid were found to be lower than reported values for the same constituents in blood and urine of rainbow trout (Salmo gairdneri). Glucose and total bilirubin were significantly correlated (P < 0.05) to hatchability, whereas LDH was negatively correlated (P < 0.05).Rainbow trout egg proteins are highly nutritional and contain a higher proportion of essential amino acids than do eggs or human or cows' milk.

scholarly journals Circulating miRNA diversity, origin and response to changing metabolic and reproductive states, new insights from the rainbow trout

2021 ◽  
Author(s):  
E Emilie Cardona ◽  
C Cervin Guyomar ◽  
Thomas Desvignes ◽  
J Jérôme Montfort ◽  
Samia Guendouz ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Egg Quality ◽  
Biological Fluids ◽  
Rna Seq ◽  
Feeding Rates ◽  
Ovarian Fluid ◽  
Non Invasive ◽  
Metabolic States ◽  
Evolutionarily Conserved ◽  
Strong Candidate

AbstractCirculating miRNAs (c-miRNAs) are found in most, if not all, biological fluids and are becoming well established biomarkers of many human pathologies. The aim of the present study was to investigate the potential of c-miRNAs as biomarkers of reproductive and metabolic states in fish, a question that has received little attention. Plasma was collected throughout the reproductive cycle from rainbow trout females subjected to two different feeding levels to trigger contrasting metabolic states; ovarian fluid was sample at ovulation. Fluid samples were subjected to small RNA-seq analysis followed by quantitative PCR validation for a subset of promising c-miRNA biomarkers. A comprehensive miRNA repertoire, which was lacking in trout, was first established to allow subsequent analysis. We first showed that biological fluids miRNAomes are complex and encompass a high proportion of the overall species miRNAome. While sharing a high proportion of common miRNAs, plasma and ovarian fluid miRNAomes exhibited strong fluid-specific signatures. We further showed that the plasma miRNAome exhibited major significant changes depending on metabolic and reproductive state. We subsequently identified three (miR-1-1/2-3p, miR-133-a-1/2-3p and miR-206-3p) evolutionarily conserved muscle-specific miRNA that accumulate in the plasma in response to high feeding rates, making these myomiRs strong candidate biomarkers of active myogenesis. We also identified miR-202-5p as a candidate biomarker for reproductive success that could be used to predict ovulation and/or egg quality. These highly promising results reveal the high potential of c-miRNAs as physiologically relevant biomarkers and pave the way for the use of c-miRNAs for non-invasive phenotyping in various fish species.

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