Protein profile study in European eel (Anguilla anguilla) seminal plasma and its correlation with sperm quality

2010 ◽  
Vol 26 (5) ◽  
pp. 746-752 ◽  
Author(s):  
D. S. Peñaranda ◽  
F. Marco-Jiménez ◽  
L. Pérez ◽  
V. Gallego ◽  
I. Mazzeo ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Protein Profile ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Profile Study

Ionic composition and physio-chemical parameters of the European eel (Anguilla anguilla) seminal plasma

2003 ◽  
Vol 28 (1-4) ◽  
pp. 221-222 ◽  
Author(s):  
L. Pérez ◽  
J.F. Asturiano ◽  
S. Martínez ◽  
A. Tomás ◽  
L. Olivares ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Ionic Composition ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Chemical Parameters

scholarly journals Sperm quality in wild-caught and farmed males of the European eel (Anguilla anguilla)

2018 ◽  
Vol 198 ◽  
pp. 167-176 ◽  
Author(s):  
L. Locatello ◽  
D. Bertotto ◽  
R. Cerri ◽  
A. Parmeggiani ◽  
N. Govoni ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Anguilla Anguilla ◽  
European Eel

scholarly journals Protein profile as a quality indicator of cryopreserved semen from Tambaqui Colossoma macropomum (Cuvier, 1818)

2020 ◽  
Vol 80 (4) ◽  
pp. 752-762
Author(s):  
J. M. Galo ◽  
D. P. Streit-Jr ◽  
C. D. Corcini ◽  
A. S. Varela-Jr ◽  
R. D. Jardim ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Protein Profile ◽  
Great Influence ◽  
Fertilization Rate ◽  
Colossoma Macropomum ◽  
One Dimensional ◽  
Qualitative And Quantitative ◽  
Sds Page ◽  
Dimensional Electrophoresis

Abstract The aim of this study was to evaluate the association between proteins in the seminal plasma of tambaqui Colossoma macropomum (Cuvier, 1818) with seminal quality indicators after thawing. The semen was cryopreserved with a dilution based on BTS with 8% DMSO. A 200 µL sample of semen from each animal was diluted in 800 µL BTS, centrifuged at 800 rpm, and the supernatant was cryopreserved to further analyze of the protein profile of seminal plasma through one-dimensional electrophoresis (SDS-PAGE). After 15 days of cryopreservation, a cryopreserved semen straw was thawed to analyze both qualitative and quantitative parameters. When considering all collections, the SDS-PAGE identified 15 protein bands in the seminal plasma of tambaqui. When the interaction (presence or absence) between proteins observed in the seminal plasma and the post thawed spermatic parameters was evaluated, we observed a great influence of the presence of proteins on spermatic quality. A greater (P<0.05) fertilization rate was observed with the presence of proteins 12, 34, 44, 85, and 90 kDa. Proteins in seminal plasma of tambaqui influenced the spermatic quality after thawing, and thus, they can be utilized as an indicator of sperm quality, especially the proteins with a molecular weight ≤ 50 kDa.

Metazoan Parasites and Health State of European Eel, Anguilla Anguilla (Anguilliformes, Anguillidae), From Tonga Lake and El Mellah Lagoon in the Northeast of Algeria

2018 ◽  
Vol 52 (4) ◽  
pp. 279-288 ◽  
Author(s):  
F. Bakaria ◽  
S. Belhaoues ◽  
N. Djebbari ◽  
M. Tahri ◽  
I. Ladjama ◽  
...  
Keyword(s):  
Brackish Water ◽  
Parasite Species ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Health State ◽  
Component Community ◽  
Metazoan Parasites ◽  
Parasite Communities ◽  
High Dominance ◽  
Argulus Foliaceus

Abstract The aim of the study was to examine metazoans parasite communities of European eels (Anguilla anguilla) in freshwater (Tonga Lake) and brackish water (El Mellah lagoon) in the northeast of Algeria. Six parasite taxa were collected: one monogenean, Pseudodactylogyrus sp.; two crustaceans, Ergasilus sp. and Argulus foliaceus; two nematodes, Cucullanus sp. and Anguillicola crassus; one cestode, Bothriocephalus claviceps. Th e most prevalent parasite taxa in freshwater were Pseudodactylogyrus sp., A. crassus and Bothriocephalus claviceps; whereas in the brackish water, eels were infected mainly with A. crassus. Th e characteristics of the parasite component community structure revealed low parasite species diversity and high dominance values in eels from the two localities. Both communities were dominated by a single parasite species: Tonga eels by the monogenean Pseudodactylogyrus sp. and El Mellah lagoon eels by the nematode A. crassus, verified by high Berger-Parker dominance values of 0.76 and 0.87 respectively.

scholarly journals Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Maja Zakošek Pipan ◽  
Petra Zrimšek ◽  
Breda Jakovac Strajn ◽  
Katarina Pavšič Vrtač ◽  
Tanja Knific ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Characteristics ◽  
Freezing And Thawing ◽  
Additional Tool ◽  
Bull Semen ◽  
Bull Sperm ◽  
Fresh Semen

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

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