Serological Evidence for the Intervention of Bovine Respiratory Syncytial Virus in a Respiratory Disease Outbreak in Moroccan Cattle

2010 ◽  
Vol 29 (1) ◽  
pp. 76-79 ◽  
Author(s):  
L. Mahin ◽  
G. Wellemans
EcoHealth ◽  
2016 ◽  
Vol 13 (3) ◽  
pp. 499-510 ◽  
Author(s):  
Kim S. Grützmacher ◽  
Sophie Köndgen ◽  
Verena Keil ◽  
Angelique Todd ◽  
Anna Feistner ◽  
...  

2017 ◽  
Vol 38 (1) ◽  
pp. 283 ◽  
Author(s):  
Selwyn Arligton Headley ◽  
Luciana Carvalho Balbo ◽  
Alice Fernandes Alfieri ◽  
João Paulo Elsen Saut ◽  
Anderson Lopes Baptista ◽  
...  

Bovine respiratory disease (BRD) is a complex multifactorial and multi-etiological disease entity that is responsible for the morbidity and mortality particularly in feedlot cattle from North America. Information relative to the occurrence of BRD in Brazil and the associated infectious agents are lacking. This study investigated the participation of infectious agents of BRD in a beef cattle feedlot from Southeastern Brazil. Nasopharyngeal swabs of 11% (10/90) of cattle (n, 450) with clinical manifestations of respiratory distress were analyzed by targeting specific genes of the principal infectious pathogens of BRD. In addition, pulmonary fragments of one the animals that died were collected for histopathological and molecular diagnoses. The nucleic acids of Histophilus somni and bovine respiratory syncytial virus (BRSV) were identified in 20% (2/10) of the nasopharyngeal swabs of the animals with respiratory distress; another contained only BRSV RNA. Moreover, the nucleic acids of both infectious agents were amplified from the pulmonary fragments of the animal that died with histopathological evidence of bronchopneumonia and interstitial pneumonia; the nasopharyngeal swab of this animal also contained the nucleic acids of both pathogens. Additionally, all PCR and/or RT-PCR assays designed to detect the specific genes of Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, bovine viral diarrhea virus, bovine herpesvirus -1, bovine parainfluenza virus-3, and bovine coronavirus yielded negative results. Phylogenetic analyses suggest that the isolates of H. somni circulating in Brazil are similar to those identified elsewhere, while there seem to be diversity between the isolates of BRSV within cattle herds from different geographical locations of Brazil.


2014 ◽  
Vol 40 (03) ◽  
pp. 123-130
Author(s):  
Shyh-Shyan Liu ◽  
Hsiu-Yen Shen ◽  
Jai-Wei Lee ◽  
Show-Win Lin ◽  
Hunter Chen ◽  
...  

A dairy farm with 300 Holstein cattle in Hsin-Chu County, Taiwan, had an outbreak of a respiratory disease from the end of September to November, 2013. Adult animals (1–5-year-old) showed clinical symptoms of anorexia, depression, fever, and dropped milk production during the early stage of infection. In severe cases, animals suffered from dyspnea with frothy saliva at the edge of opened mouth. Samples were collected from eight sick animals, amplified in the baby hamster kidney cell-21 (BHK-21), and the cytopathic effect (CPE) was confirmed. Primers specific to Bovine respiratory syncytial virus (BRSV), Bovine viral diarrhea virus (BVDV), Bovine herpesvirus type I (BHV-I), Bovine ephemeral fever virus (BEFV) and Mannheimia haemolytica were used to identify the pathogen responsible for this respiratory disease by polymerase chain reaction (PCR). Results of sequence analysis confirmed that BRSV is the causative pathogen for the respiratory infection. This is, to the best of our knowledge, the first disease case of BRSV reported in Taiwan.


2000 ◽  
Vol 31 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Mauro Costa ◽  
Laura Garc�a ◽  
Abdul S. Yunus ◽  
Daniel D. Rockemann ◽  
Siba K. Samal ◽  
...  

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