Codetection of Respiratory Syncytial Virus in Habituated Wild Western Lowland Gorillas and Humans During a Respiratory Disease Outbreak

We have examined the function of class I MHC-restricted cytotoxic T cells in experimental respiratory syncytial virus (RSV) infection of BALB/c mice by transfer of T cell line MJC-A2 and CTL clone E8a into RSV-infected mice. The T cell line cleared pulmonary RSV infection within 5 d in persistently infected gamma-irradiated mice, but caused acute respiratory disease. This was only seen in infected mice and was often lethal after transfer of greater than 3 x 10(6) CTL. Lower numbers of CTL produced less severe disease but still cleared lung RSV, albeit over a longer time course (up to 10 d). Clearance of lung RSV in immunocompetent mice by the T cell line and CTL clone was again accompanied by acute and sometimes lethal respiratory disease. Bronchoalveolar lavage showed severe lung hemorrhage and frequent neutrophil efflux in mice with CTL-augmented disease.

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Temporal Dysbiosis of Infant Nasal Microbiota Relative to Respiratory Syncytial Virus Infection

The Journal of Infectious Diseases ◽

10.1093/infdis/jiaa577 ◽

2020 ◽

Author(s):

Alex Grier ◽

Ann L Gill ◽

Haeja A Kessler ◽

Anthony Corbett ◽

Sanjukta Bandyopadhyay ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Respiratory Disease ◽

Disease Severity ◽

Temporal Dynamics ◽

Disease Risk ◽

Developmental Trajectory ◽

Microbiota Composition ◽

Rsv Infection ◽

Syncytial Virus ◽

Nasal Microbiota

Abstract Rationale Respiratory Syncytial Virus (RSV) is a leading cause of infant respiratory disease. Infant airway microbiota has been associated with respiratory disease risk and severity. The extent to which interactions between RSV and microbiota occur in the airway, and their impact on respiratory disease susceptibility and severity, are unknown. Objectives Characterize temporal associations between microbiota and RSV infection before, during, and after infants’ first respiratory illness. Methods 16S rRNA microbiota profiling of two infant cohorts in the first year of life: 1) a cross-sectional cohort of 89 RSV infected infants sampled during illness and 102 matched healthy controls, and 2) a matched longitudinal cohort of 12 infants who developed RSV infection and 12 who did not, sampled before, during, and after infection. Results We identified 12 taxa significantly associated with RSV infection. All 12 taxa were differentially abundant during infection, with 8 associated with disease severity. Nasal microbiota composition was more discriminative of healthy vs. infected than of disease severity. Conclusions Our findings elucidate the chronology of nasal microbiota dysbiosis and suggest an altered developmental trajectory associated with RSV infection. Microbial temporal dynamics reveal indicators of disease risk, correlates of illness and severity, and impact of RSV infection on microbiota composition.

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The effect of formalin-inactivated vaccine on respiratory disease associated with bovine respiratory syncytial virus infection in calves

Vaccine ◽

10.1016/s0264-410x(98)00265-5 ◽

1999 ◽

Vol 17 (7-8) ◽

pp. 809-820 ◽

Cited By ~ 39

Author(s):

Keith West ◽

Lyall Petrie ◽

Deborah M Haines ◽

Carrie Konoby ◽

Edward G Clark ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Virus Infection ◽

Respiratory Disease ◽

Respiratory Syncytial Virus Infection ◽

Bovine Respiratory Syncytial Virus ◽

Inactivated Vaccine ◽

Syncytial Virus

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Observations on outbreaks of respiratory disease in calves associated with parainfluenza type 3 virus and respiratory syncytial virus infection

Veterinary Record ◽

10.1136/vr.104.3.45 ◽

1979 ◽

Vol 104 (3) ◽

pp. 45-49 ◽

Cited By ~ 31

Author(s):

D. Bryson ◽

J. McFerran ◽

H. Ball ◽

S. Neill

Keyword(s):

Respiratory Syncytial Virus ◽

Virus Infection ◽

Respiratory Disease ◽

Respiratory Syncytial Virus Infection ◽

Syncytial Virus ◽

Type 3

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Bovine respiratory disease associated with Histophilus somni and bovine respiratory syncytial virus in a beef cattle feedlot from Southeastern Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n1p283 ◽

2017 ◽

Vol 38 (1) ◽

pp. 283 ◽

Cited By ~ 3

Author(s):

Selwyn Arligton Headley ◽

Luciana Carvalho Balbo ◽

Alice Fernandes Alfieri ◽

João Paulo Elsen Saut ◽

Anderson Lopes Baptista ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Beef Cattle ◽

Nucleic Acids ◽

Respiratory Distress ◽

Respiratory Disease ◽

Bovine Respiratory Syncytial Virus ◽

Southeastern Brazil ◽

Infectious Agents ◽

Histophilus Somni ◽

Syncytial Virus

Bovine respiratory disease (BRD) is a complex multifactorial and multi-etiological disease entity that is responsible for the morbidity and mortality particularly in feedlot cattle from North America. Information relative to the occurrence of BRD in Brazil and the associated infectious agents are lacking. This study investigated the participation of infectious agents of BRD in a beef cattle feedlot from Southeastern Brazil. Nasopharyngeal swabs of 11% (10/90) of cattle (n, 450) with clinical manifestations of respiratory distress were analyzed by targeting specific genes of the principal infectious pathogens of BRD. In addition, pulmonary fragments of one the animals that died were collected for histopathological and molecular diagnoses. The nucleic acids of Histophilus somni and bovine respiratory syncytial virus (BRSV) were identified in 20% (2/10) of the nasopharyngeal swabs of the animals with respiratory distress; another contained only BRSV RNA. Moreover, the nucleic acids of both infectious agents were amplified from the pulmonary fragments of the animal that died with histopathological evidence of bronchopneumonia and interstitial pneumonia; the nasopharyngeal swab of this animal also contained the nucleic acids of both pathogens. Additionally, all PCR and/or RT-PCR assays designed to detect the specific genes of Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, bovine viral diarrhea virus, bovine herpesvirus -1, bovine parainfluenza virus-3, and bovine coronavirus yielded negative results. Phylogenetic analyses suggest that the isolates of H. somni circulating in Brazil are similar to those identified elsewhere, while there seem to be diversity between the isolates of BRSV within cattle herds from different geographical locations of Brazil.

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