Double-sandwich Enzyme-linked Immunosorbent Assay (ELISA) with Murine Monoclonal Antibodies for Detection of Staphylococcal Enterotoxin B

1994 ◽  
Vol 41 (1-10) ◽  
pp. 639-644
Author(s):  
J. Goyache ◽  
J. A. Orden ◽  
J. L. Blanco ◽  
A. Doménech ◽  
G. Suárez ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Staphylococcal Enterotoxin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B ◽  
Murine Monoclonal Antibodies

scholarly journals Measurement of Staphylococcal Enterotoxin B in Serum and Culture Supernatant with a Capture Enzyme-Linked Immunosorbent Assay

2007 ◽  
Vol 14 (9) ◽  
pp. 1094-1101 ◽  
Author(s):  
E. Cook ◽  
X. Wang ◽  
N. Robiou ◽  
B. C. Fries
Keyword(s):  
Immunosorbent Assay ◽  
Staphylococcal Enterotoxin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Staphylococcal Enterotoxin B ◽  
Low Concentrations ◽  
Life Threatening ◽  
Nasal Inhalation ◽  
Enterotoxin B ◽  
Rapid Binding

ABSTRACT Staphylococcal enterotoxin B (SEB) is a select agent because it is a potent mitogen that elicits life-threatening polyclonal T-cell proliferation and cytokine production at very low concentrations. Efforts are in progress to develop therapeutic reagents and vaccines that neutralize or prevent the devastating effects of this toxin. Because of its rapid binding to in vivo receptors, this toxin is difficult to detect in serum. This rapid binding also constitutes a major challenge for the development of effective therapeutic reagents that can neutralize the effects of the toxin in vivo. We have developed a highly sensitive capture enzyme-linked immunosorbent assay that detects SEB in body fluids at very low levels. With this assay, the peak levels of SEB in serum and renal clearance can be measured in mice. After either oral ingestion or nasal inhalation of SEB by mice, this assay documents the transcytosis of SEB across the mucosal membranes into serum within 2 h. Furthermore, this assay was used to compare the SEB levels in different murine models for SEB-induced lethal shock and demonstrated that the coadministration of toxin-enhancing chemicals, such as d-galactosamine and lipopolysaccharide, can alter the peak serum SEB levels. Hence, this assay is a potentially useful tool for the study of the pharmacokinetics of SEB and the effects of potential therapeutic reagents on serum SEB levels.

Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization

1992 ◽  
Vol 89 (5) ◽  
pp. 247-254 ◽  
Author(s):  
Joaquín Goyache ◽  
JoséA. Orden ◽  
JoséL. Blanco ◽  
Javier Hernández ◽  
Ana Doménech ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B ◽  
Murine Monoclonal Antibodies

Effect of Thermal Processing during Yogurt Production upon the Detection of Staphylococcal Enterotoxin B

2009 ◽  
Vol 72 (10) ◽  
pp. 2212-2216 ◽  
Author(s):  
MARYANN PRINCIPATO ◽  
THOMAS BOYLE ◽  
JOYCE NJOROGE ◽  
ROBERT L. JONES ◽  
MICHAEL O'DONNELL
Keyword(s):  
Storage Period ◽  
Staphylococcal Enterotoxin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Staphylococcal Enterotoxin B ◽  
Type I ◽  
Type Ii ◽  
Biochemical Characteristics ◽  
Enterotoxin B ◽  
Study Type ◽  
Physical Changes

This research was conducted to examine the inherent properties of yogurt contaminated with staphylococcal enterotoxin B (SEB). Two types of yogurts were produced for this study. Type I yogurts were produced by adding SEB at the start of yogurt production, and type II yogurts were produced by adding SEB after the milk base had been boiled. Biochemical characteristics inherent to yogurt, including pH, lactic acid and acetaldehyde concentrations, were analyzed weekly for each batch beginning at a time just after production and throughout a storage period of at least 4 weeks. The presence of toxin during yogurt production did not result in any significant biochemical or physical changes in yogurt. However, we were unable to detect SEB toxin in type I yogurt using a commercially available enzyme-linked immunosorbent assay (ELISA). In contrast, SEB was easily detectable by our ELISA in type II yogurt samples. Higher levels of SEB were recovered from type II yogurt that had been stored for 1 week than from type II yogurt that had been stored for any other length of time. These results indicate that the biochemical characteristics of yogurt did not change significantly (relative to control yogurt) in the presence of either thermally processed SEB or native SEB. However, the ability to detect SEB by ELISA was dependent on whether the toxin had been processed.

scholarly journals Mechanisms Mediating Enhanced Neutralization Efficacy of Staphylococcal Enterotoxin B by Combinations of Monoclonal Antibodies

2015 ◽  
Vol 290 (11) ◽  
pp. 6715-6730 ◽  
Author(s):  
Kaushik Dutta ◽  
Avanish K. Varshney ◽  
Matthew C. Franklin ◽  
Michael Goger ◽  
Xiaobo Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B

scholarly journals Production of Specific Monoclonal Antibodies toAspergillus Species and Their Use in Immunohistochemical Identification of Aspergillosis

1999 ◽  
Vol 37 (4) ◽  
pp. 1221-1223 ◽  
Author(s):  
L. E. Fenelon ◽  
A. J. Hamilton ◽  
J. I. Figueroa ◽  
M. A. Bartholomew ◽  
M. H. Allen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoperoxidase Staining ◽  
Frozen Sections ◽  
Clinical Specimens ◽  
Murine Monoclonal Antibodies ◽  
Immunohistochemical Identification

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.

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