Defective Polymorphonuclear Neutrophil Function in Dairy Cows Showing Enhanced Susceptibility to Intramammary Infections

1995 ◽  
Vol 42 (1-10) ◽  
pp. 625-632 ◽  
Author(s):  
R. Cooray ◽  
L. Håkansson
Keyword(s):  
Dairy Cows ◽  
Neutrophil Function ◽  
Polymorphonuclear Neutrophil ◽  
Intramammary Infections

The neutrophil function and lymphocyte profile of milk from bovine mammary glands infected with Streptococcus dysgalactiae

2015 ◽  
Vol 82 (4) ◽  
pp. 460-469 ◽  
Author(s):  
Maiara G Blagitz ◽  
Fernando N Souza ◽  
Camila F Batista ◽  
Luis Fernando F Azevedo ◽  
Nilson Roberti Benites ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dairy Cows ◽  
Mammary Glands ◽  
Neutrophil Function ◽  
Negative Control ◽  
Streptococcus Dysgalactiae ◽  
T Lymphocyte Subsets ◽  
Intramammary Infections ◽  
Cell Counts ◽  
Culture Negative

Streptococcus dysgalactiaeis a bacterium that accounts for a notable proportion of both clinical and subclinical intramammary infections (IMIs). Thus, the present study explores the function of milk neutrophils and the lymphocyte profile in mammary glands naturally infected withStreptococcus dysgalactiae. Here, we used 32 culture-negative control quarters from eight clinically healthy dairy cows with low somatic cell counts and 13S. dysgalactiae-infected quarters from six dairy cows. Using flow cytometry, we evaluated the percentage of milk monocytes/macrophages and neutrophils, expression of CD62L, CD11b and CD44 by milk neutrophils, the levels of intracellular reactive oxygen species (ROS) production and phagocytosis ofStaphylococcus aureusby milk neutrophils, and neutrophil viability. Furthermore, the percentages of B cell (CD21+) and T lymphocyte subsets (CD3+/CD4+/CD8−; CD3+/CD8+/CD4−; and CD3+/CD8−/CD4−), and the expression of CD25 by T milk lymphocytes (CD3+) and T CD4+milk cells were also assessed by flow cytometry using monoclonal antibodies. The present study showed a higher SCC and percentage of milk neutrophils, and a decrease in the percentage of milk monocytes/macrophages fromS. dysgalactiae-infected quarters when compared to uninfected ones. We also observed a higher expression of CD11b by milk neutrophils and a tendency toward a decrease in neutrophil apoptosis rate inS. dysgalactiae-infected quarters. In addition, theS. dysgalactiae-infected quarters had higher percentages of milk T cells (CD3+) and their subset CD3+CD8+CD4−cells. Overall, the present study provided new insights intoS. dysgalactiaeIMIs, including distinct lymphocyte profiles, and a tendency toward an inhibition of apoptosis in milk neutrophils.

scholarly journals Blood immunometabolic indices and polymorphonuclear neutrophil function in peripartum dairy cows are altered by level of dietary energy prepartum

2012 ◽  
Vol 95 (4) ◽  
pp. 1749-1758 ◽  
Author(s):  
D.E. Graugnard ◽  
M. Bionaz ◽  
E. Trevisi ◽  
K.M. Moyes ◽  
J.L. Salak-Johnson ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Neutrophil Function ◽  
Polymorphonuclear Neutrophil ◽  
Dietary Energy

Association of metabolic markers with neutrophil function in healthy postpartum dairy cows

2021 ◽  
Vol 232 ◽  
pp. 110182
Author(s):  
O. Bogado Pascottini ◽  
T.C. Bruinjé ◽  
R. Couto Serrenho ◽  
B. Mion ◽  
S.J. LeBlanc
Keyword(s):  
Dairy Cows ◽  
Neutrophil Function ◽  
Metabolic Markers

Acquired abnormalities of polymorphonuclear neutrophil function

Blood Reviews ◽  
1990 ◽  
Vol 4 (2) ◽  
pp. 103-110 ◽  
Author(s):  
J. Fletcher ◽  
A.P. Haynes ◽  
S.M. Crouch
Keyword(s):  
Neutrophil Function ◽  
Polymorphonuclear Neutrophil ◽  
Acquired Abnormalities

scholarly journals Comparative efficacy of antimicrobial treatments in dairy cows at dry-off to prevent new intramammary infections during the dry period or clinical mastitis during early lactation: a systematic review and network meta-analysis

2019 ◽  
Vol 20 (2) ◽  
pp. 199-216 ◽  
Author(s):  
C. B. Winder ◽  
J. M. Sargeant ◽  
D. Hu ◽  
C. Wang ◽  
D. F. Kelton ◽  
...  
Keyword(s):  
Systematic Review ◽  
Dairy Cows ◽  
Meta Analysis ◽  
Clinical Mastitis ◽  
Comparative Efficacy ◽  
Dry Period ◽  
Quality Of Reporting ◽  
Intramammary Infections ◽  
Antimicrobial Treatments

AbstractA systematic review and network meta-analysis were conducted to assess the relative efficacy of antimicrobial therapy given to dairy cows at dry-off. Eligible studies were controlled trials assessing the use of antimicrobials compared to no treatment or an alternative treatment, and assessed one or more of the following outcomes: incidence of intramammary infection (IMI) at calving, incidence of IMI during the first 30 days in milk (DIM), or incidence of clinical mastitis during the first 30 DIM. Databases and conference proceedings were searched for relevant articles. The potential for bias was assessed using the Cochrane Risk of Bias 2.0 algorithm. From 3480 initially identified records, 45 trials had data extracted for one or more outcomes. Network meta-analysis was conducted for IMI at calving. The use of cephalosporins, cloxacillin, or penicillin with aminoglycoside significantly reduced the risk of new IMI at calving compared to non-treated controls (cephalosporins, RR = 0.37, 95% CI 0.23–0.65; cloxacillin, RR = 0.55, 95% CI 0.38–0.79; penicillin with aminoglycoside, RR = 0.42, 95% CI 0.26–0.72). Synthesis revealed challenges with a comparability of outcomes, replication of interventions, definitions of outcomes, and quality of reporting. The use of reporting guidelines, replication among interventions, and standardization of outcome definitions would increase the utility of primary research in this area.

scholarly journals Reducing the incidence of acute puerperal metritis in primiparous cows by application of pegbovigrastim in a Holstein dairy herd

2018 ◽  
Vol 63 (No. 4) ◽  
pp. 151-160 ◽  
Author(s):  
M. Freick ◽  
M. Zenker ◽  
O. Passarge ◽  
J. Weber
Keyword(s):  
Milk Fat ◽  
Animal Health ◽  
Dairy Herd ◽  
Neutrophil Function ◽  
Milk Composition ◽  
Clinical Mastitis ◽  
Polymorphonuclear Neutrophil ◽  
Control Group ◽  
Relative Reduction ◽  
Beneficial Effects

In dairy cows, immunosuppression occurs frequently during the periparturient period and is characterised by transient neutropenia and impaired polymorphonuclear neutrophil function. As a consequence, postpartum cows are more susceptible to production-related diseases such as mastitis, retained foetal membranes and metritis. Recently, it has been shown that a double injection with recombinant bovine granulocyte colony-stimulating factor covalently bound to polyethylene glycol (PEG rbG-CSF) increased polymorphonuclear neutrophil number and the exocytosis of myeloperoxidase by stimulating polymorphonuclear neutrophil, which also played a role in innate uterine immune defence. The aim of this randomised clinical study was to investigate the effects of two periparturient PEG rbG-CSF injections (IMR; Imrestor<sup>TM</sup>, Elanco Animal Health, Bad Homburg/Germany) on the incidence of acute puerperal metritis, number of antibiotic doses required for treatment of acute puerperal metritis as well as parameters of milking performance (i.e., milk yield, milk fat, milk protein, and somatic cell count on the first dairy herd improvement test day) in primiparous cows within a dairy herd with an elevated incidence of acute puerperal metritis. In total, 169 heavily pregnant heifers were randomly assigned to the treatment group (IMR: n = 82) who received 15 mg PEG rbG-CSF subcutaneously 10 ± 3 days before the anticipated calving date and within 24 hours after calving, or to the untreated control group (Co: n = 87). In total, data from 157 animals (IMR: n = 75, Co: n = 82) were analysed. Administration of PEG rbG-CSF reduced the incidence of acute puerperal metritis in primiparous cows significantly (IMR: 22.7%, Co: 43.9%, P = 0.003; relative reduction: 48.3%). Moreover, the number of antibiotic doses per calving required for treatment of acute puerperal metritis was significantly lower in the pegbovigastrim group (IMR: 0.32 ± 0.66, Co: 0.59 ± 0.75, P = 0.005). No significant differences regarding incidence of clinical mastitis, milk production or milk composition were observed. These results suggest that further research should be performed to identify herd- and animal-specific factors that can be predictors of the beneficial effects of pegbovigrastim in preventing uterine diseases.

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