Dynamic distribution of endoplasmic reticulum in hippocampal neuron dendritic spines

2005 ◽  
Vol 22 (7) ◽  
pp. 1793-1798 ◽  
Author(s):  
Håkan Toresson ◽  
Seth G. N. Grant
Keyword(s):  
Endoplasmic Reticulum ◽  
Dendritic Spines ◽  
Hippocampal Neuron ◽  
Dynamic Distribution

scholarly journals Synaptic anchoring of the endoplasmic reticulum depends on myosin V and caldendrin activity

2020 ◽  
Author(s):  
Anja Konietzny ◽  
Jasper Grendel ◽  
Nathalie Hertrich ◽  
Dick H. W. Dekkers ◽  
Jeroen A. A. Demmers ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Motor Function ◽  
Light Chain ◽  
Dendritic Spines ◽  
Hippocampal Neurons ◽  
Molecular Diversity ◽  
Fine Tuning ◽  
Excitatory Synapses ◽  
Myosin V ◽  
Spine Apparatus

AbstractExcitatory synapses of principal hippocampal neurons are frequently located on dendritic spines. The dynamic strengthening or weakening of individual inputs results in a great structural and molecular diversity of dendritic spines. Active spines with large Ca2+ transients are frequently invaded by a single protrusion from the endoplasmic reticulum (ER), which is dynamically transported into and out of spines by the actin-based motor myosin V. An increase in synaptic strength often correlates with stable anchoring of the ER, followed by the formation of the spine apparatus organelle. Here we show that synaptic ER stabilization depends on the interplay of two Ca2+-binding proteins: calmodulin serves as a light chain of myosin V and activates the motor function, whereas caldendrin acts as an inhibitor which transforms myosin into a stationary F-actin tether. Together, they provide a Ca2+-sensing module for fine-tuning myosin V activity and thereby regulate the formation of the spine apparatus in a subset of active dendritic spines.

scholarly journals Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation

2014 ◽  
Vol 7 (1) ◽  
Author(s):  
Ai Na Ng ◽  
Andrew J Doherty ◽  
Paul J Lombroso ◽  
Nigel J Emptage ◽  
Graham L Collingridge
Keyword(s):  
Endoplasmic Reticulum ◽  
Nmda Receptor ◽  
Dendritic Spines ◽  
Receptor Activation ◽  
Nmda Receptor Activation

scholarly journals Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons

2010 ◽  
Vol 13 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Wolfgang Wagner ◽  
Stephan D. Brenowitz ◽  
John A. Hammer
Keyword(s):  
Endoplasmic Reticulum ◽  
Dendritic Spines ◽  
Purkinje Neurons ◽  
Myosin Va

Organization of intracellular calcium regulatory proteins in the neuronal endoplasmic reticulum

1994 ◽  
Vol 52 ◽  
pp. 26-27
Author(s):  
Maryann E. Martone ◽  
Victoria M. Edelman ◽  
Saul A. Alba ◽  
Thomas J. Deerinck ◽  
Mark H. Ellisman
Keyword(s):  
Endoplasmic Reticulum ◽  
Intracellular Calcium ◽  
Dendritic Spines ◽  
Calcium Binding ◽  
Smooth Endoplasmic Reticulum ◽  
Membrane System ◽  
Regulatory Proteins ◽  
Purkinje Neurons ◽  
Storage Site ◽  
Ip3 Receptor

The smooth endoplasmic reticulum (SER) has been established as an intracellular calcium storage site in neurons. Although the SER appears to form a continuous membrane system within neurons, immunolocalization studies suggest that calcium regulatory proteins are not evenly distributed within the SER but are selectively concentrated or excluded from certain domains. The subcompartmenalization of the SER has been clearly demonstrated in Purkinje neurons where two proteins involved in the release of calcium from intracellular stores, the IP3 and ryanodine receptor, were differentially localized within dendrites. Both proteins were found associated with the SER in cell bodies and dendrites of chick Purkinje neurons but only labeling for the IP3 receptor was found within dendritic spines. A similar differential localization was described in Purkinje cell dendrites for the SER Ca++ATPase and calsequestrin, a lumenal calcium binding protein. The Ca++ATPase was found throughout dendrites and dendritic spines while calsequestrin was restricted to membranous profiles within the dendritic shaft.

scholarly journals Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Alberto Perez-Alvarez ◽  
Shuting Yin ◽  
Christian Schulze ◽  
John A. Hammer ◽  
Wolfgang Wagner ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Dendritic Spines ◽  
Low Frequency ◽  
Pyramidal Cells ◽  
Long Term Potentiation ◽  
Dominant Negative ◽  
Synaptic Activity ◽  
Small Subset ◽  
Myosin V

Abstract In hippocampal pyramidal cells, a small subset of dendritic spines contain endoplasmic reticulum (ER). In large spines, ER frequently forms a spine apparatus, while smaller spines contain just a single tubule of smooth ER. Here we show that the ER visits dendritic spines in a non-random manner, targeting spines during periods of high synaptic activity. When we blocked ER motility using a dominant negative approach against myosin V, spine synapses became stronger compared to controls. We were not able to further potentiate these maxed-out synapses, but long-term depression (LTD) was readily induced by low-frequency stimulation. We conclude that the brief ER visits to active spines have the important function of preventing runaway potentiation of individual spine synapses, keeping most of them at an intermediate strength level from which both long-term potentiation (LTP) and LTD are possible.

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