scholarly journals Contribution of Selectins to Leucocyte Sequestration in Pulmonary Microvessels by Intravital Microscopy in Rabbits

1997 ◽  
Vol 501 (2) ◽  
pp. 375-386 ◽  
Author(s):  
W. M. Kuebler ◽  
G. E. H. Kuhnle ◽  
J. Groh ◽  
A. E. Goetz
Keyword(s):  
Intravital Microscopy ◽  
Pulmonary Microvessels

Measuring pulmonary microvessel diameters using video image analysis

1995 ◽  
Vol 79 (2) ◽  
pp. 526-532 ◽  
Author(s):  
C. C. Hanger ◽  
S. C. Hillier ◽  
R. G. Presson ◽  
R. W. Glenny ◽  
W. W. Wagner
Keyword(s):  
Image Analysis ◽  
Intravital Microscopy ◽  
Video Image ◽  
Video Image Analysis ◽  
Heuristic Technique ◽  
Pulmonary Microvessels ◽  
Relationship Of

To directly determine the pressure-diameter relationship of individual pulmonary microvessels, it is necessary to measure the width of the column of blood in the vessel because microvascular walls are invisible when using intravital microscopy. To identify the margins of the blood column accurately, we developed a method for computer enhancement and measurement of vessel images. After recording microvessels on videotape, consecutive frames from the videotape were digitized by a computer. Pixels that changed from frame to frame (moving erythrocytes) were turned white, and unchanging pixels were turned black. In this way an image of the erythrocyte column with distinct edges was produced. The width of this column was measured with a heuristic technique involving interactions between the computer and the user. The measurements were reproducible and accurate. This technique has been used to measure microvascular diameters over a range of well-defined microvascular pressures and construct precise pressure-diameter curves.

scholarly journals 77286 Intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis

2021 ◽  
pp. 1-2
Author(s):  
Emmanuel Gabriel ◽  
Minhyung Kim ◽  
Daniel Fisher ◽  
Catherine Mangum ◽  
Kristopher Attwood ◽  
...  
Keyword(s):  
Peritoneal Carcinomatosis ◽  
Intravital Microscopy ◽  
Tumor Vasculature

scholarly journals A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Emmanuel M. Gabriel ◽  
Minhyung Kim ◽  
Daniel T. Fisher ◽  
Catherine Mangum ◽  
Kristopher Attwood ◽  
...  
Keyword(s):  
Peritoneal Carcinomatosis ◽  
Neoadjuvant Therapy ◽  
Anticancer Agents ◽  
Intravital Microscopy ◽  
Pilot Trial ◽  
Oncologic Outcomes ◽  
Tumor Control ◽  
Systemic Delivery ◽  
Tumor Microvasculature ◽  
Lower Blood

AbstractAberrancies in the tumor microvasculature limit the systemic delivery of anticancer agents, which impedes tumor response. Using human intravital microscopy (HIVM), we hypothesized that HIVM would be feasible in patients with peritoneal carcinomatosis (PC). During cytoreductive surgery with hyperthermic intraperitoneal chemotherapy for PC, HIVM was performed in both tumor and non-tumor areas. The primary outcome was HIVM feasibility to measure vessel characteristics. We secondarily evaluated associations between HIVM vessel characteristics and oncologic outcomes (RECIST response to neoadjuvant therapy and disease-specific survival). Thirty patients with PC were enrolled. Nineteen patients (63.3%) received neoadjuvant therapy. HIVM was feasible in all patients. Compared to non-tumor (control) areas, PC areas had a lower density of functional vessels, higher proportion of non-functional vessels, smaller lumenal diameters, and lower blood flow velocity. Qualitative differences in these vessel characteristics were observed among patients who had partial response, stable disease, or progressive disease after receiving neoadjuvant therapy. However, no statistically significant relationships were found between HIVM vessel characteristics and oncologic outcomes. These novel findings comprise the first-in-human, real-time evidence of the microscopic differences between normal and tumor-associated vessels and form the basis for our larger, ongoing clinical trial appropriately powered to determine the clinical utility of HIVM (NCT03823144).

scholarly journals Intravital microscopy imaging of kidney injury and regeneration

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yue Liu ◽  
Zongjin Li
Keyword(s):  
Cell Fate ◽  
Intravital Microscopy ◽  
Single Cells ◽  
Elevated Serum ◽  
Kidney Injury ◽  
Lineage Tracing ◽  
Tissue Slices ◽  
Microscopy Imaging ◽  
Two Photon Microscopy ◽  
Nitrogen Levels

AbstractAcute kidney injury (AKI) is a common clinical symptom, which is mainly manifested by elevated serum creatinine and blood urea nitrogen levels. When AKI is not repaired in time, the patient is prone to develop chronic kidney disease (CKD). The kidney is composed of more than 30 different cells, and its structure is complex. It is extremely challenging to understand the lineage relationships and cell fate of these cells in the process of kidney injury and regeneration. Since the 20th century, lineage tracing technology has provided an important mean for studying organ development, tissue damage repair, and the differentiation and fate of single cells. However, traditional lineage tracing methods rely on sacrificing animals to make tissue slices and then take snapshots with conventional imaging tools to obtain interesting information. This method cannot achieve dynamic and continuous monitoring of cell actions on living animals. As a kind of intravital microscopy (IVM), two-photon microscopy (TPM) has successfully solved the above problems. Because TPM has the ability to penetrate deep tissues and can achieve imaging at the single cell level, lineage tracing technology with TPM is gradually becoming popular. In this review, we provided the key technical elements of lineage tracing, and how to use intravital imaging technology to visualize and quantify the fate of renal cells.

Late Effects of TNF-α-Induced Inflammation on the Microcirculation of Cremaster Muscle Flaps under Intravital Microscopy

2002 ◽  
Vol 18 (1) ◽  
pp. 037-046 ◽  
Author(s):  
Kagan Ozer ◽  
Gokhan Adanali ◽  
Maria Siemionow
Keyword(s):  
Intravital Microscopy ◽  
Late Effects ◽  
Cremaster Muscle ◽  
Muscle Flaps ◽  
Tnf Α
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