Morphological aspects of in vitro co-cultivation of Frankia alni subsp. pommerii Lalonde (strain ACN1AG) and Frankia elaeagni (Schroter) Becking (strain EANlpec) with larch (Larix x eurolepis Henry) cells

Previous studies conducted with the imidazolidinic derivative 3-benzyl-5-(4-chloro-arylazo)-4-thioxo-imidazolidin-2-one (LPSF-PT05) show outstanding activity against adultSchistosoma mansoniwormsin vitro. In the first phase of this study,S. mansoni-infected mice were treated, orally, with 100 mg/Kg of the LPSF-PT05 in three formulations: Tween 80 and saline solution, oil/water (70 : 30) emulsion, and solid dispersion with polyethylene glycol (PEG). In the second phase, three other doses of the LPSF-PT05 in PEG were tested: 3, 10, 30 mg/kg. These treatment regimens significantly reduced the number of recovered worms due to increases in the solubility of the compound in this formulation; the greatest reduction (70.5%) was observed at the dose of 100 mg/kg. There was no changes in the pattern of mature egg compared to immature eggs; however there was a significant increase in the number of dead eggs. Histopathological analysis of liver tissue showed changes in morphological aspects of the hepatic parenchyma with decrease exudative-productive hepatic granuloma stages, although we found no significant differences in IFN-γ, IL-4, IL-10, or NO production in response to the specific antigen SEA. The results show the derivative LPSF-PT05 to be a potential candidate in the etiological treatment of schistosomiasis with a possible dampening effect of the granulomatous process.

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Morphological aspects of fruits, seeds, seedlings and in vivo and in vitro germination of species of the genus Cleome

Journal of Seed Science ◽

10.1590/2317-1545v36n31013 ◽

2014 ◽

Vol 36 (3) ◽

pp. 326-335 ◽

Cited By ~ 1

Author(s):

Tatiana Carvalho de Castro ◽

Claudia Simões-Gurgel ◽

Ivan Gonçalves Ribeiro ◽

Marsen Garcia Pinto Coelho ◽

Norma Albarello

Keyword(s):

Urban Areas ◽

Native Species ◽

Human Impacts ◽

In Vitro Germination ◽

Morphological Aspects ◽

Physiological Dormancy ◽

Cleome Spinosa ◽

The Tropics

The genus Cleome is widely distributed in drier areas of the tropics and subtropics. Cleome dendroides and C. rosea are Brazilian native species that occur mainly in Atlantic Forest and sandy coastal plains, respectively ecosystems negatively affected by human impacts. Cleome spinosa is frequently found in urban areas. Many Cleome species have been used in traditional medicine, as C. spinosa. In the present work, was investigated C. dendroides, C. rosea and C. spinosa germinative behavior under in vivo conditions, as well as was established suitable conditions to in vitro germination and seedling development. The in vivo germination was performed evaluating the influence of temperature, substrate and light. It was observed that only C. spinosa seeds presents physiological dormancy, which was overcome by using alternate temperatures. The substrate influenced significantly the germination of C. rosea and the seeds of C. dendroides showed the highest germination percentages in the different conditions evaluated. The post-seminal development stages under in vivo and in vitro conditions were defined. It was observed that the development was faster under in vitro than in vivo conditions. An effective methodology for in vitro germination, enabling the providing of material to experiment on plant tissue culture was established to C. dendroides and C. spinosa.

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Morphological aspects of human lymphoid cells grown in vitro

In Vitro ◽

10.1007/bf02661724 ◽

1972 ◽

Vol 7 (5) ◽

pp. 344-358 ◽

Cited By ~ 1

Author(s):

Benjamin Drewinko ◽

Jose M. Trujillo

Keyword(s):

Lymphoid Cells ◽

Morphological Aspects

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5-Fluorouracil Release from Chitosan-Based Matrix.Experimental and Theoretical Aspects

Materiale Plastice ◽

10.37358/mp.20.3.5392 ◽

2020 ◽

Vol 57 (3) ◽

pp. 180-188

Author(s):

Roxana Iancu ◽

Stefan Andrei Irimiciuc ◽

Maricel Agop ◽

Mihail Frasila ◽

Maria-Alexandra Paun ◽

...

Keyword(s):

Drug Release ◽

Polarized Light ◽

Crosslinking Density ◽

Fickian Diffusion ◽

Release Mechanism ◽

Morphological Aspects ◽

Drug Release Mechanism ◽

The Matrix

A series of four drug release formulations based on 5-fluorouracil encapsulated into a chitosan-based matrix were prepared by in situ hydrogelation with 3,7-dimethyl-2,6-octadienal. The formulations were investigated from structural and morphological aspects by FTIR spectroscopy, polarized light microscopy and scanning electron microscopy. It was established that 5-fluorouracil was anchored into the matrix as crystals, whose dimension varied as a function of the crosslinking density. The in vitro drug release simulated into a media mimicking the physiological environment revealed a progressive release of the 5-fluorouracil, in close interdependence with the crosslinking density. In the context of Pharmacokinetics behavioral analysis, a new mathematical procedure for describing drug release dynamics in polymer-drug complex system is proposed. Assuming that the dynamics of polymer-drug system�s structural units take place on continuous and nondifferentiable curves (multifractal curves), we show that in a one-dimensional hydrodynamic formalism of multifractal variables the drug release mechanism (Fickian diffusion, non-Fickian diffusion, etc) are given through synchronous dynamics at a differentiable and non-differentiable scale resolutions. Finally, the model is confirmed by the empirical data.

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IL-17A activated by Toll-like receptor 9 contributes to the development of septic acute kidney injury

AJP Renal Physiology ◽

10.1152/ajprenal.00313.2019 ◽

2020 ◽

Vol 318 (1) ◽

pp. F238-F247

Author(s):

Yoshitaka Naito ◽

Takayuki Tsuji ◽

Soichiro Nagata ◽

Naoko Tsuji ◽

Tomoyuki Fujikura ◽

...

Keyword(s):

Acute Kidney Injury ◽

Critical Role ◽

Cecal Ligation ◽

Kidney Injury ◽

Mrna Levels ◽

Toll Like Receptor ◽

Γδt Cells ◽

Morphological Aspects ◽

Septic Acute Kidney Injury

Toll-like receptor 9 (TLR9), which is activated by endogenously released mtDNA during sepsis, contributes to the development of polymicrobial septic acute kidney injury (AKI). However, downstream factors of TLR9 to AKI remain unknown. We hypothesized that IL-17A activated by TLR9 may play a critical role in septic AKI development. To determine the effects of TLR9 on IL-17A production in septic AKI, we used a cecal ligation and puncture (CLP) model in Tlr9 knockout ( Tlr9KO) mice and wild-type (WT) littermates. We also investigated the pathway from TLR9 activation in dendritic cells (DCs) to IL-17A production by γδT cells in vitro. To elucidate the effects of IL-17A on septic AKI, Il-17a knockout ( Il-17aKO) mice and WT littermates were subjected to CLP. We further investigated the relationship between the TLR9-IL-17A axis and septic AKI by intravenously administering recombinant IL-17A or vehicle into Tlr9KO mice and assessing kidney function. IL-17A levels in both plasma and the peritoneal cavity and mRNA levels of IL-23 in the spleen were significantly higher in WT mice after CLP than in Tlr9KO mice. Bone marrow-derived DCs activated by TLR9 induced IL-23 and consequently promoted IL-17A production in γδT cells in vitro. Knockout of Il-17a improved survival, functional and morphological aspects of AKI, and splenic apoptosis after CLP. Exogenous IL-17A administration aggravated CLP-induced AKI attenuated by knockout of Tlr9. TLR9 in DCs mediated IL-17A production in γδT cells during sepsis and contributed to the development of septic AKI.

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