scholarly journals Discrepancies in identification of Vibrio cholerae strains as members of Aeromonadaceae and Enterobacteriaceae by automated microbial identification system

2012 ◽  
Vol 55 (1) ◽  
pp. 22-26 ◽  
Author(s):  
A. Saini ◽  
H. Kaur ◽  
S. Purwar ◽  
S. D. Kholkute ◽  
S. Roy
Keyword(s):  
Vibrio Cholerae ◽  
Identification System ◽  
Microbial Identification ◽  
Microbial Identification System

Isolation and Identification of Bacillus from Spontaneous Fermented Sufu

2013 ◽  
Vol 634-638 ◽  
pp. 1179-1183 ◽  
Author(s):  
Jing Deng ◽  
Hua Chang Wu ◽  
Xing Xiu Zhao ◽  
Jiao Jiao Shi
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Amyloliquefaciens ◽  
Fermented Food ◽  
Identification System ◽  
Isolation And Identification ◽  
Spontaneous Fermentation ◽  
Fermentation Products ◽  
Microbial Identification ◽  
Microbial Identification System

Sufu is a traditional Chinese fermented food. The safety of spontaneous fermentation products has been concerned by more and more people. A total of four isolates with big clear halos on the casein medium plate were isolated from spontaneous fermented Sufu in southern Sichuan. A1 and A3 most likely belong to Bacillus cereus B according to their phenotype and Biolog Microbial Identification System. B2 was classified in group as Bacillus amyloliquefaciens B with the same methods. A2 was identified as Bacillus subtilis according to their phenotype and 16SrRNA. The safety of the strains are also discussed.

scholarly journals Evaluation of the Biolog automated microbial identification system.

1992 ◽  
Vol 58 (6) ◽  
pp. 2089-2092 ◽  
Author(s):  
J M Klingler ◽  
R P Stowe ◽  
D C Obenhuber ◽  
T O Groves ◽  
S K Mishra ◽  
...  
Keyword(s):  
Identification System ◽  
Microbial Identification ◽  
Microbial Identification System

scholarly journals Bacterial Stem Rot of Poinsettia Caused by a Dickeya sp. (Pectobacterium chrysanthemi) in China

Plant Disease ◽  
2008 ◽  
Vol 92 (7) ◽  
pp. 1135-1135 ◽  
Author(s):  
K. Rungnapha ◽  
S. H. Yu ◽  
G. L. Xie
Keyword(s):  
Xanthomonas Campestris ◽  
Methyl Esters ◽  
Similarity Index ◽  
Stem Rot ◽  
Unknown Etiology ◽  
Identification System ◽  
Bacterial Strains ◽  
Microbial Identification ◽  
Pectobacterium Chrysanthemi ◽  
Microbial Identification System

In December 2006, a rot symptom of unknown etiology was observed on stems of plants (Euphorbia pulcherrima cv. Fu-xing) at a flower nursery in the Zhejiang Province of China where we had previously reported leaf spot of poinsettia caused by Xanthomonas campestris (2). Chlorotic spots anywhere along the stem and purplish black petioles were the first noticeable symptoms. The spots rapidly coalesced, forming large irregular chlorotic areas. Petioles turned black and shriveled and affected leaves wilted. Infected tissues were soft and water soaked. Ten bacterial strains were isolated from the diseased samples and five were selected for identification. They were similar to those of the standard reference strains of Pectobacterium chrysanthemi (Dickeya sp.), LMG 2804 from Belgium and ZUPB20056 from China, in phenotypic tests based on the Biolog Microbial Identification System, version 4.2 (Biolog Inc., Hayward, CA), pathogenicity tests, gas chromatography of fatty acid methyl esters (FAME) using the Microbial Identification System (MIDI Inc, Newark, DE) with aerobic bacterial library (TABA50), and transmission electron microscopy (TEM,KYKY-1000B, Japan). All strains tested were gram-negative facultative anaerobic rods measuring 1.5 to 3.6 × 0.6 to 1.1 μm, with peritrichous flagella. Colonies were gray-white and slightly raised with smooth margins on nutrient agar. They were negative for trehalose and positive for phosphatase production and reducing substances from sucrose. A hypersensitive reaction was observed on tobacco cv. Benshi, 24 h after inoculation. All five isolates, LMG 2804, and ZUPB20056 were identified as P. chrysanthemi (Dickeya sp.) with a Biolog similarity index of 0.58 to 0.83, 0.68, and 0.72 and a FAME similarity index of 0.52 to 0.80, 0.59, and 0.70, respectively. Identification as P. chrysanthemi (Dickeya sp.) was confirmed by PCR with specific primers used by Nassar et al (3). Koch's postulates were completed with the inoculation of 12 4-month-old intact poinsettia plants of cv. Fu-xing with cell suspensions containing 108 CFU/ml by a pinprick at the base of the stem. All five strains induced stem infection similar to those observed in natural infections. No symptoms were noted on the two control plants inoculated with sterilized distilled water by the same method. The bacterium was reisolated from symptomatic stems of poinsettia plants. P. chrysanthemi (Dickeya sp.) was first reported in United States as the cause of bacterial stem rot of poinsettia in 1972 (1). To our knowledge, this is the first report of poinsettia stem rot caused by P. chrysanthemi (Dickeya sp.) in China. The disease cycle and the control strategies of the bacterial stem rot of poinsettia in the regions are being further studied. References: (1) H. A. J. Hoitink et al. Plant Dis. Rep. 56:480, 1972. (2) B. Li et al. Plant Pathol. 55:293, 2006. (3) A. A. Nassar et al. Appl. Environ. Microbiol. 62:2228, 1996.

scholarly journals First Report of Pear Blossom Blast Caused by Pseudomonas syringae pv. syringae in China

Plant Disease ◽  
2008 ◽  
Vol 92 (5) ◽  
pp. 832-832 ◽  
Author(s):  
L. H. Xu ◽  
G. L. Xie ◽  
B. Li ◽  
B. Zhu ◽  
F. S. Xu ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Similarity Index ◽  
Nutrient Agar ◽  
Identification System ◽  
Pyrus Pyrifolia ◽  
Rrna Gene ◽  
Bacterial Disease ◽  
First Report ◽  
Microbial Identification ◽  
Microbial Identification System

In the spring of 2006, a new bacterial disease was noted in pear orchards near Hangzhou, Zhejiang Province, China. The disease caused severe blossom blast on pears (Pyrus pyrifolia; cv. Cuiguan). Early symptoms of the disease included blackening of the calyx end of developing fruit, blackening of blossom clusters while leaves of affected blossom clusters appeared normal, or death of clusters consisting of both blossoms and leaves. Later, tips of twigs turned dark brown and died. No bacterial ooze was observed. Twelve bacterial isolates were recovered from ten samples of buds and blossoms. Six isolates were selected for identification. They were similar to those of the reference strains of Pseudomonas syringae pv. syringae LMG5570 and LMG 2230 from Belgium in phenotypic tests on the basis of the Biolog Microbial Identification System (version 4.2; Biolog Inc., Hayward, CA), pathogenicity tests, gas chromatographic analysis of fatty acid methyl esters (FAMEs) using the Microbial Identification System (MIDI Inc., Newark, DE) with aerobic bacterial library (TABA50), and electron microscopy (TEM, KYKY-1000B, Japan). All isolates tested were gram-negative, aerobic rods measuring 1.5 to 2.4 × 0.5 to 0.6 μm with 2 to 4 polar flagella. Fluorescent green diffusible pigment was produced on King's Medium B. Colonies were gray-white and slightly raised with smooth margins on nutrient agar. They produced levan on sucrose nutrient agar. A hypersensitive reaction was observed on tobacco cv. Benshi 24 h after inoculation. All isolates were identified as P. syringae pv. syringae with Biolog similarity index of 0.57 to 0.86 and FAME similarity index of 0.58 to 0.81. Identification as P. syringae pv. syringae was confirmed using 16S rDNA universal primers (2,3): 5′-AGA GTT TGA TCA TGG CTC AG-3′ forward primer, 5′-ACG GTT ACC TTG TTA CGA CTT-3′ reverse primer. The PCR fragments of the three isolates were sequenced and compared with sequences in GenBank. They had 99% similiarity with P. syringae pv. syringae 16S rRNA gene strain NCPPB 3869. Koch's postulates were conducted on buds of the original pear cultivar growing in pots and detached pear blossoms in flasks by spray inoculation with cell suspensions containing 108 CFU/ml of the six isolates at 18 to 22°C with two replications. The bacteria induced symptoms on buds and blossoms similar to those observed in the field. The bacterium was reisolated from symptomatic pear buds and internal ovary tissues. P. syringae pv. syringae was first reported in England as the cause of pear blossom blast in 1914 (1). After searching all the Chinese agricultural databases and major journals (National Knowledge Infrastructure database, Vip Chinese periodical database, Chinese wanfang database, China InfoBank, Scientia Agricultura Sinica, Acta Phytopathologica Sinica, Acta Phytophylacica Sinica, and Journal of Fruit Science), to our knowledge, this is the first report of pear blossom blast caused by P. syringae pv. syringae in China. The disease cycle on pear trees and the control strategies in the regions are being further studied. References: (1) B. P. Barker et al. Ann. Appl. Biol. 1:85, 1914. (2) U. Edward et al. Nucleic Acids Res. 17:7843,1989. (3) B. Li et al. J. Phytopathol. 34:141, 2006.

scholarly journals First Report of a Field Outbreak of a Bacterial Leaf Spot of Cantaloupe and Squash Caused by Pseudomonas syringae pv. syringae in Georgia

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 600-600 ◽  
Author(s):  
D. B. Langston ◽  
F. H. Sanders ◽  
J. H. Brock ◽  
R. D. Gitaitis ◽  
J. T. Flanders ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Tap Water ◽  
Acid Methyl Ester ◽  
Economic Damage ◽  
Identification System ◽  
First Report ◽  
Microbial Identification ◽  
Microbial Identification System ◽  
Physiological Tests

In March 2000, a leaf spot was reported affecting yellow summer squash (Cucurbita pepo) and cantaloupe (Cucumis melo) in commercial fields in Colquitt, Echols, and Grady counties in Georgia. All of the crops affected were reported within a 10-day period, and average temperatures during that time were 8 to 22.5°C, which is very close to the 50-year normal temperatures for these areas located in southwest Georgia. Incidence in affected fields was 100%. Lesions on squash leaves appeared irregularly shaped, dark, water soaked, somewhat vein restricted, and were 5 to 10 mm in diameter. Lesions on cantaloupe were angular, light tan, and necrotic with a lesion diameter of 2 to 5 mm. A general chlorosis was observed around lesions of both crops. Leaf distortion was observed on squash. Four isolates collected were used in biochemical, pathogenicity, and physiological tests. Gram-negative, rod-shaped bacteria were isolated from diseased tissue from squash and cantaloupes. Bacteria were aerobic, catalase-positive, fluorescent on King's medium B (1), oxidase-negative, nonpectolytic on potato, arginine dihydrolase-negative, utilized sucrose as a carbon source, produced levan, and gave a hypersensitivity response on tobacco (HR). Analysis of fatty acid methyl ester (FAME) profiles using the Microbial identification system (Sherlock version 3.1, Microbial identification system, Newark, DE) characterized representative strains as Pseudomonas syringae (similarity indices 0.65 to 0.80). Upon further characterization, the strains were negative for l (+)-tartarate utilization but utilized l-lactate and betaine and also exhibited ice nucleation activity. These characteristics are consistent with those of Pseudomonas syringae pv. syringae. Squash and cantaloupes were grown in a greenhouse for 4 weeks. Bacteria were grown in nutrient broth, resuspended in sterile tap water, and standardized using a spectrophotometer. Plants were inoculated by infiltrating leaves with 1 ml of bacterial suspensions (1 × 107 CFU/ml) using sterile syringes. Sterile water was used as a negative control, and 1 ml was infiltrated into leaves of squash and cantaloupes. Water-soaked lesions developed in 4 to 6 days on squash and cantaloupes inoculated with bacterial suspensions, and Pseudomonas syringae pv. syringae was isolated from diseased tissue. No symptoms developed on squash and cantaloupes used as negative controls. This outbreak of Pseudomonas syringae pv. syringae did not cause significant economic damage to either crop as symptoms subsided once daily high temperatures reached 28 to 32°C. This disease has been isolated from several cucurbit transplants reared in greenhouses, but to our knowledge, this is the first report of this disease occurring in the field. Reference: (1) E. O. King et al. J. Lab. Clin. Med. 44:301, 1954.

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