EFFECTS OF DIVALENT AND LANTHANIDE IONS ON MOTILITY INITIATION IN RAT CAUDAL EPIDIDYMAL SPERMATOZOA

Fluoride crystals, due to their low phonon energies, are attractive hosts of trivalent lanthanide ions for applications in upconverting phosphors, quantum information science, and solid-state laser refrigeration. In this article, we report the rapid, low-cost hydrothermal synthesis of potassium lutetium fluoride (KLF) microcrystals for applications in solid-state laser refrigeration. Four crystalline phases were synthesized, namely orthorhombic K2LuF5 (Pnma), trigonal KLuF4 (P3121), orthorhombic KLu2F7 (Pna21), and cubic KLu3F10 (Fm3m), with each phase exhibiting unique microcrystalline morphologies. Luminescence spectra and emission lifetimes of the four crystalline phases were characterized based on the point-group symmetry of trivalent cations. Laser refrigeration was measured by observing both the optomechanical eigenfrequencies of microcrystals on cantilevers in vacuum, and also the Brownian dynamics of optically trapped microcrystals in water. Among all four crystalline phases, the most significant cooling was observed for 10%Yb:KLuF4 with cooling of 8.6 $\pm$ 2.1 K below room temperature. Reduced heating was observed with 10%Yb:K2LuF5

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Bond-length distributions for ions bonded to oxygen: Results for the lanthanides and actinides and discussion of the f-block contraction

10.26434/chemrxiv.5410924.v3 ◽

2017 ◽

Author(s):

Olivier Charles Gagné

Keyword(s):

Bond Length ◽

Coordination Number ◽

Lanthanide Ions ◽

Lanthanide Contraction ◽

Trivalent Lanthanide ◽

Actinide Ions

Bond-length distributions have been examined for eighty-four configurations of the lanthanide ions and twenty-two configurations of the actinide ions bonded to oxygen. The lanthanide contraction for the trivalent lanthanide ions bonded to O2- is shown to vary as a function of coordination number and to diminish in scale with increasing coordination number.

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Assessment of Pomegranate Juice as Antioxidant in Extender on Cauda Epididymis Spermatozoal Quality of Buck at Refrigerated Temperature

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.15.2.7 ◽

2019 ◽

Vol 15 (02) ◽

pp. 26-29

Author(s):

Amarjeet Amarjeet ◽

C T Khasatiya ◽

L Chaudhary

Keyword(s):

Detrimental Effect ◽

Egg Yolk ◽

Antioxidant Effect ◽

Pomegranate Juice ◽

Antioxidant Additive ◽

Epididymal Spermatozoa ◽

The Dead

The present investigation was carried out to study the refrigeration preservation of the cauda epididymal retrieved spermatozoa of buck in Tris egg yolk citrate (TEYC) dilutor containing pomegranate juice as antioxidant additive. The retrieved cauda epididymal spermatozoa extended in TEYC dilutor were studied in five groups by adding different concentration of pomegranate juice as additive (0% as control T1 group and 5%, 10%, 15% and 20% as treatment T2, T3, T4 and T5 groups, respectively) and storing at refrigerated temperature up to 48 hr. The results showed that the control extender had the least dead, abnormal and HOS non-reacted sperm percent among all treatments tested and that with increasing the pomegranate juice concentration in dilutor, the percentage of the dead, abnormal and HOST non-reacted spermatozoa increased significantly. The same trend was observed at all 12 hourly storage intervals indicating its detrimental effect on epididymal sperms of bucks at refrigeration temperature. The dead, abnormal, and HOST non-reacted sperm were significantly and positively interrelated with each other (r = 0.53-0.83). It was concluded that the inclusion of pomegranate juice in TEYC dilutor did not show any beneficial/antioxidant effect on epididymal sperms of buck in fresh or refrigerated semen and in fact all the levels of pomegranate juice (5% to 20%) were detrimental to cauda epididymal spermatozoa of a buck.

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Effect of Tomato Juice in Tris-Yolk-Citrate Extender onRefrigeration Preservation of Cauda Epididymal Spermatozoa of Buck

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.15.1.17 ◽

2019 ◽

Vol 15 (01) ◽

pp. 71-74

Author(s):

L M Chaudhary ◽

C T Khasatiya ◽

Amarjeet Amarjeet ◽

A B Yede

Keyword(s):

Adverse Effect ◽

Egg Yolk ◽

Physical Characteristics ◽

Tomato Juice ◽

The Mean ◽

Epididymal Spermatozoa ◽

Sperm Traits

The study was carried out on the preservation of epididymal spermatozoa of buck at refrigerated temperature without and with tomato juice as a supplement in Tris egg yolk citrate extender. The eight pairs of testicles including epididymis (total 16) from slaughtered bucks were collected within 2–4 hours of their slaughter. Sperms collected from cauda epididymis were preserved at refrigerated temperature up to 48 hours in tris egg yolk citrate extender at 300 million sperm/mL with different concentration of tomato juice (0%, 4%, 6%, 8%, and 10%) and the physical characteristics of spermatozoa were assessed to know the effect of tomato juice (Tj). The mean dead, abnormal and HOST non-reacted spermatozoa increased significantly (p less than0.05) at every 12-hour intervals of preservation in the dilutor without and with different concentration of tomato juice. Tomato juice exerted an adverse effect on physical characteristics of sperm during refrigeration preservation. All the three sperm traits studied however revealed significant (p less thaN 0.01) positive interrelationships with correlations of 0.31 to 0.72.

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Azaoxa Macrocyclic and Acyclic Complexesof Lanthanides

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19980363 ◽

1998 ◽

Vol 63 (3) ◽

pp. 363-370 ◽

Cited By ~ 13

Author(s):

Violetta Patroniak-Krzyminiewska ◽

Wanda Radecka-Paryzek

Keyword(s):

Schiff Base ◽

Spectral Data ◽

Macrocyclic Ligand ◽

Lanthanide Ions ◽

H Nmr ◽

Elemental Analyses ◽

Acyclic Complexes ◽

Schiff Base Cyclocondensation

The template reactions of 2,6-diacetylpyridine with 3,6-dioxaoctane-1,8-diamine in the presence of dysprosium(III), thulium(III) and lutetium(III) chlorides and erbium(III) perchlorate produce the complexes of 15-membered macrocyclic ligand with an N3O2 set of donor atoms as a result of the [1+1] Schiff base cyclocondensation. In contrast, analogous reactions involving the lighter lanthanide ions (lanthanum(III), samarium(III) and europium(III)) yield the acyclic complexes with terminal acetylpyridyl groupings as products of the partial [2+1] condensation. The complexes were characterized by spectral data (IR, UV-VIS, 1H NMR, MS), and thermogravimetric and elemental analyses.

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The effect of terminal N-donor aromatic ligands on sensitization and emission of lanthanide ions in Zn2Ln (Ln = Eu, Tb) complexes with 4-biphenylcarboxylate anions

New Journal of Chemistry ◽

10.1039/d0nj05994c ◽

2021 ◽

Author(s):

Stanislav N. Melnikov ◽

Igor Evstifeev ◽

Stanislav A. Nikolaevskii ◽

Ivan V. Ananyev ◽

Evgenia Varaksina ◽

...

Keyword(s):

General Formula ◽

Molecular Complexes ◽

Lanthanide Ions ◽

Biphenylcarboxylic Acid

The systematical series of Zn-Ln trinuclear carboxylate molecular complexes with general formula [Zn2Ln(NO3)(phbz)6(L)2] (Ln = Eu, Gd, Tb; phbz is anion of 4-biphenylcarboxylic acid; L is pyridine, 2,3-lutidine or 2,2’-bipyridine)...

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E3 Ubiquitin Ligase SPL2 Is a Lanthanide-Binding Protein

International Journal of Molecular Sciences ◽

10.3390/ijms22115712 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5712

Author(s):

Michał Tracz ◽

Ireneusz Górniak ◽

Andrzej Szczepaniak ◽

Wojciech Białek

Keyword(s):

Ubiquitin Ligase ◽

Conformational Changes ◽

Protein Interactions ◽

E3 Ubiquitin Ligase ◽

Lanthanide Ions ◽

E3 Ligases ◽

Fluorescence Measurements ◽

Partial Unfolding

The SPL2 protein is an E3 ubiquitin ligase of unknown function. It is one of only three types of E3 ligases found in the outer membrane of plant chloroplasts. In this study, we show that the cytosolic fragment of SPL2 binds lanthanide ions, as evidenced by fluorescence measurements and circular dichroism spectroscopy. We also report that SPL2 undergoes conformational changes upon binding of both Ca2+ and La3+, as evidenced by its partial unfolding. However, these structural rearrangements do not interfere with SPL2 enzymatic activity, as the protein retains its ability to auto-ubiquitinate in vitro. The possible applications of lanthanide-based probes to identify protein interactions in vivo are also discussed. Taken together, the results of this study reveal that the SPL2 protein contains a lanthanide-binding site, showing for the first time that at least some E3 ubiquitin ligases are also capable of binding lanthanide ions.

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