Analysis of the in vitro synergistic effect of 5-fluorouracil and cisplatin on cervical carcinoma cells

2006 ◽  
Vol 16 (3) ◽  
pp. 1321-1329 ◽  
Author(s):  
E.-K. YIM ◽  
S.B. LEE ◽  
K.-H. LEE ◽  
C.-J. KIM ◽  
J.-S. PARK
2006 ◽  
Vol 16 (3) ◽  
pp. 1321-1329 ◽  
Author(s):  
E. K. Yim ◽  
S. B. Lee ◽  
K. H. Lee ◽  
C. J. Kim ◽  
J. S. Park

5-Fluorouracil (5-FU) is currently being used as an anticancer drug to reduce tumor bulk in order to increase the operability rate and postoperative survival in patients with cervical cancer, which has been combined with cisplatin (CP) because of its superior activities observed in human carcinoma cells. However, the combined anticancer effect of 5-FU and CP in cervical carcinoma cells is poorly understood. Therefore, we conducted a study to investigate whether anticancer drugs 5-FU and CP may exhibit the combined antiproliferative effect in cervical carcinoma cells. Using proteomics analysis, including two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), we investigated the antiproliferative effect–related proteins after treatment with 5-FU and/or CP. Our experiments showed that the combination of 5-FU and CP engaged both the apoptotic pathways: the membrane death receptor–mediated apoptosis pathway and the mitochondrial apoptotic pathway. Moreover, the combination of 5-FU and CP resulted in remarkable increasing susceptibility to apoptosis. We suggest that the combination of 5-FU and CP suppresses the growth of cervical carcinoma cells by synergistic effect with the induction of apoptosis. In vitro synergistic effect of 5-FU and CP supports the basis of the clinical application of the combination chemotherapy to the patients with cervical cancer.


2020 ◽  
Vol 10 (8) ◽  
pp. 1213-1217
Author(s):  
Jinghua Xiao ◽  
Yani Li ◽  
Yanxia Wang ◽  
Xinru Wang ◽  
Wanwan Zhang ◽  
...  

This study aims to investigate the killing effect of γ-Fe2O3 combined with carmustine on cervical carcinoma cells, as well as the mechanism of drug resistance of this combined treatment. RT-PCR was used to detect the expression of Multidrug Resistance Gene (MDR-l) gene in all the samples. Results showed that the combination of γ-Fe2O3 and carmustine drugs was significantly better than the control group (P < 0.05), and the tumor showed different degrees of tolerance in combination with the drug. The results of an in vitro drug sensitivity test were consistent with those of mRNA expression of MDR-l gene (P < 0.04). The combination of superparamagnetic γ-Fe2O3 and carmustine derivatives has a better therapeutic effect on cervical carcinoma than either treatment alone. The drug sensitivity test and MDR-l mRNA detection in vitro can improve the accuracy and predictability of cervical carcinoma chemotherapy, providing a scientific basis for individual tumor chemotherapy.


2019 ◽  
Vol 33 (1) ◽  
pp. 1599-1607
Author(s):  
Mingcheng Li ◽  
Gang Wang ◽  
Ruowen Zhang ◽  
Siqi Duan ◽  
Jiayu Chen

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