Tight junction protein claudin-6 inhibits growth and induces the apoptosis of cervical carcinoma cells in vitro and in vivo

Abstract Background: Weaning stress can lead to the disruption of tight junctions and increased intestinal permeabilty, which contributes to the initiation and development of many disease, such as Crohn’s disease and ulcerative colitis. Pigs are more ideal models for human studies than other animals. However, no information is found about the relationship of intestinal integrity and spermine supplementation in pigs. The objective of this study is to investigate whether spermine protects intestinal barrier integrity via Rac1/PLC-γ1 signalling pathway in piglets. Methods: In vivo, the piglets were categorised into the control group and the spermine group, which was fed with spermine at 0.4 mmol kg−1 body weight for 7 hours and 3, 6 and 9 days. In vitro, we examined whether spermine protects the intestinal barrier after TNF-α challenge through Ras-related C3 botulinum toxin substrate 1 (Rac1)/Phospho-lipase C-γ1 (PLC-γ1) signalling pathway. Results: In vivo study revealed that the spermine treatment upregulated tight junction protein mRNA levels and Rac1/PLC-γ1 signalling pathway gene expression in the jejunum of piglets. The serum D-lactate content was significantly reduced after spermine treatment (P < 0.05). In vitro study revealed that 0.1 μM spermine significantly increased the levels of tight junction protein expression, Rac1/PLC-γ1 signalling pathway and transepithelial electrical resistance, and decreased paracellular permeability (P < 0.05). Further experiments showed that spermine treatment increased the levels of tight junction protein expression, Rac1/PLC-γ1 signalling pathway and transepithelial electrical resistance, and decreased paracellular permeability compared with the NSC-23766 and U73122 treatment with spermine after TNF-α challenge (P < 0.05). Conclusion: spermine protects intestinal integrity through the Rac1/PLC-γ1 signalling pathway.

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Meprin A impairs epithelial barrier function, enhances monocyte migration, and cleaves the tight junction protein occludin

AJP Renal Physiology ◽

10.1152/ajprenal.00179.2012 ◽

2013 ◽

Vol 305 (5) ◽

pp. F714-F726 ◽

Cited By ~ 21

Author(s):

Jialing Bao ◽

Renee E. Yura ◽

Gail L. Matters ◽

S. Gaylen Bradley ◽

Pan Shi ◽

...

Keyword(s):

Tight Junction ◽

Barrier Function ◽

Fluorescein Isothiocyanate ◽

Tight Junction Protein ◽

Sodium Fluorescein ◽

Monocyte Migration ◽

Junction Protein ◽

Epithelial Barriers

Meprin metalloproteases are highly expressed at the luminal interface of the intestine and kidney and in certain leukocytes. Meprins cleave a variety of substrates in vitro, including extracellular matrix proteins, adherens junction proteins, and cytokines, and have been implicated in a number of inflammatory diseases. The linkage between results in vitro and pathogenesis, however, has not been elucidated. The present study aimed to determine whether meprins are determinative factors in disrupting the barrier function of the epithelium. Active meprin A or meprin B applied to Madin-Darby canine kidney (MDCK) cell monolayers increased permeability to fluorescein isothiocyanate-dextran and disrupted immunostaining of the tight junction protein occludin but not claudin-4. Meprin A, but not meprin B, cleaved occludin in MDCK monolayers. Experiments with recombinant occludin demonstrated that meprin A cleaves the protein between Gly100 and Ser101 on the first extracellular loop. In vivo experiments demonstrated that meprin A infused into the mouse bladder increased the epithelium permeability to sodium fluorescein. Furthermore, monocytes from meprin knockout mice on a C57BL/6 background were less able to migrate through an MDCK monolayer than monocytes from their wild-type counterparts. These results demonstrate the capability of meprin A to disrupt epithelial barriers and implicate occludin as one of the important targets of meprin A that may modulate inflammation.

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Changes in tight junction protein expression in intrinsic aging and photoaging in human skin in vivo

Journal of Dermatological Science ◽

10.1016/j.jdermsci.2016.07.002 ◽

2016 ◽

Vol 84 (1) ◽

pp. 99-101 ◽

Cited By ~ 10

Author(s):

Seon-Pil Jin ◽

Sang Bum Han ◽

Yeon Kyung Kim ◽

Elizabeth Eunkyung Park ◽

Eun Jin Doh ◽

...

Keyword(s):

Protein Expression ◽

Tight Junction ◽

Human Skin ◽

Tight Junction Protein ◽

Tight Junction Protein Expression ◽

Junction Protein

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Hyaluronan 35 kDa treatment protects mice from Citrobacter rodentium infection and induces epithelial tight junction protein ZO-1 in vivo

Matrix Biology ◽

10.1016/j.matbio.2016.11.001 ◽

2017 ◽

Vol 62 ◽

pp. 28-39 ◽

Cited By ~ 11

Author(s):

Yeojung Kim ◽

Sean P. Kessler ◽

Dana R. Obery ◽

Craig R. Homer ◽

Christine McDonald ◽

...

Keyword(s):

Tight Junction ◽

Tight Junction Protein ◽

Citrobacter Rodentium ◽

Junction Protein ◽

Epithelial Tight Junction

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Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

Reproduction ◽

10.1530/rep-07-0572 ◽

2008 ◽

Vol 135 (6) ◽

pp. 867-877 ◽

Cited By ~ 38

Author(s):

Gerard A Tarulli ◽

Sarah J Meachem ◽

Stefan Schlatt ◽

Peter G Stanton

Keyword(s):

Tight Junction ◽

Adaptor Protein ◽

Tight Junction Protein ◽

Mrna Levels ◽

Tight Junction Proteins ◽

Djungarian Hamster ◽

Junction Protein ◽

Protein Localisation ◽

Junction Proteins

This study aimed to assess the effect of gonadotrophin suppression and FSH replacement on testicular tight junction dynamics and blood–testis barrier (BTB) organisation in vivo, utilising the seasonal breeding Djungarian hamster. Confocal immunohistology was used to assess the cellular organisation of tight junction proteins and real-time PCR to quantify tight junction mRNA. The effect of tight junction protein organisation on the BTB permeability was also investigated using a biotin-linked tracer. Tight junction protein (claudin-3, junctional adhesion molecule (JAM)-A and occludin) localisation was present but disorganised after gonadotrophin suppression, while mRNA levels (claudin-11, claudin-3 and occludin) were significantly (two- to threefold) increased. By contrast, both protein localisation and mRNA levels for the adaptor protein zona occludens-1 decreased after gonadotrophin suppression. FSH replacement induced a rapid reorganisation of tight junction protein localisation. The functionality of the BTB (as inferred by biotin tracer permeation) was found to be strongly associated with the organisation and localisation of claudin-11. Surprisingly, JAM-A was also recognised on spermatogonia, suggesting an additional novel role for this protein in trans-epithelial migration of germ cells across the BTB. It is concluded that gonadotrophin regulation of tight junction proteins forming the BTB occurs primarily at the level of protein organisation and not gene transcription in this species, and that immunolocalisation of the organised tight junction protein claudin-11 correlates with BTB functionality.

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Geniposide protects against hypoxia/reperfusion-induced blood-brain barrier impairment by increasing tight junction protein expression and decreasing inflammation, oxidative stress, and apoptosis in an in vitro system

European Journal of Pharmacology ◽

10.1016/j.ejphar.2019.04.021 ◽

2019 ◽

Vol 854 ◽

pp. 224-231 ◽

Cited By ~ 9

Author(s):

Changxiang Li ◽

Xueqian Wang ◽

Fafeng Cheng ◽

Xin Du ◽

Juntang Yan ◽

...

Keyword(s):

Oxidative Stress ◽

Protein Expression ◽

Tight Junction ◽

Blood Brain Barrier ◽

Tight Junction Protein ◽

Brain Barrier ◽

In Vitro System ◽

Tight Junction Protein Expression ◽

Junction Protein

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Correlation of occludin protein mobility with paracellular leak pathway permeability in renal epithelia

10.1101/437996 ◽

2018 ◽

Author(s):

Josephine Axis ◽

Alexander L. Kolb ◽

Robert L. Bacallao ◽

Kurt Amsler

Keyword(s):

Tight Junction ◽

Ischemia Reperfusion Injury ◽

Mdck Cells ◽

Ischemia Reperfusion ◽

Paracellular Permeability ◽

Tight Junction Protein ◽

Protein Mobility ◽

Junction Protein ◽

Leak Pathway

ABSTRACTStudies have demonstrated regulation of the epithelial paracellular permeability barrier, the tight junction, by a variety of stimuli. Recent studies have reported a correlation between changes in paracellular permeability, particularly paracellular permeability to large solutes (leak pathway), and mobility of the tight junction protein, occludin, in the plane of the plasma membrane. This had led to the hypothesis that changes in occludin protein mobility are causative for changes in paracellular permeability. Using a renal epithelial cell model system, MDCK, we examined the effect of various manipulations on both leak pathway permeability, monitored as the paracellular movement of a fluorescent molecule (calcein), and occludin protein mobility, monitored through fluorescence recovery after photobleaching. Our results indicate that knockdown of the associated tight junction protein, ZO-1, increases baseline leak pathway permeability, whereas, knockdown of the related tight junction protein, ZO-2, does not alter baseline leak pathway permeability. Knockdown of either ZO-1 or ZO-2 decreases the rate of movement of occludin protein but only knockdown of ZO-2 protein alters the percent of occludin protein that is mobile. Further, treatment with hydrogen peroxide increases leak pathway permeability in wild type MDCK cells and in ZO-2 knockdown MDCK cells but not in ZO-1 knockdown MDCK cells. This treatment decreases the rate of occludin movement in all three cell lines but only alters the mobile fraction of occludin protein in ZO-1 knockdown MDCK cells. Finally, we examined the effect of renal ischemia/reperfusion injury on occludin protein mobility in vivo.Ischemia/reperfusion injury both increased the rate of occludin mobility and increased the fraction of occludin protein that is mobile. These results indicate that, at least in our cell culture and in vivo model systems, there is no consistent correlation between paracellular leak pathway permeability and occludin protein mobility.

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