High-intensity electric pulses induce mitochondria-dependent apoptosis in ovarian cancer xenograft mice
2008 ◽
Vol 18
(6)
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pp. 1258-1261
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Keyword(s):
Cyt C
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Human ovarian cancer models were established in nude mice by transplanting SKOV3 cells, and then tumors were exposed to high-intensity electric pulses with a voltage 1000 V, frequency of 1000 Hz, and duration of 250 ns for 1 min. Mitochondria permeability transition pore (PTP) was inspected by cofocal microscope; cytochrome C (Cyt C) and apoptosis-induced factor (AIF) were determined by immunohistochemistry; and voltage-dependent anion channel (VDAC) was measured by immunofluorescence. High-intensity electric pulses exposure led to increases of PTP, Cyt C, and AIF and a decrease of VDAC. These findings revealed that high-intensity electric pulses activated mitochondria electroporation, apoptosis was realized via mitochondria pathway.
2003 ◽
Vol 278
(50)
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pp. 49812-49818
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1998 ◽
Vol 258
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pp. 729-735
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2019 ◽
Vol 316
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pp. C449-C455
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Vol 289
(4)
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pp. C994-C1001
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Vol 2013
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pp. 1-10
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Vol 1
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pp. 367-375
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Vol 289
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pp. 29285-29296
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