scholarly journals Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus

2018 ◽  
Vol 19 (10) ◽  
pp. 3013 ◽  
Author(s):  
Trinh Thuy Tien ◽  
Hyun Park ◽  
Hien Tuong ◽  
Seung-Taek Yu ◽  
Du-Young Choi ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rt Pcr ◽  
Immunochromatographic Strip ◽  
Copy Numbers ◽  
Rsv Infection ◽  
Immunochromatographic Strip Test ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Strip Test ◽  
Europium Nanoparticles

Human respiratory syncytial virus (RSV) is one of the most common viruses infecting the respiratory tracts of infants. The rapid and sensitive detection of RSV is important to minimize the incidence of infection. In this study, novel monoclonal antibodies (mAbs; B11A5 and E8A11) against RSV nucleoprotein (NP) were developed and applied to develop a rapid fluorescent immunochromatographic strip test (FICT), employing europium nanoparticles as the fluorescent material. For the FICT, the limits of detection of the antigen and virus were 1.25 µg/mL and 4.23 × 106 TCID50/mL, respectively, corresponding to 4.75 × 106 ± 5.8 ×105 (mean ± SD) RNA copy numbers per reaction mixture for RSV NP. A clinical study revealed a sensitivity of 90% (18/20) and specificity of 98.18% (108/110) for RSV detection when comparing the performance to that of reverse transcription polymerase chain reaction (RT-PCR), representing a 15% improvement in sensitivity over the SD Bioline rapid kit. This newly developed FICT could be a useful tool for the rapid diagnosis of RSV infection.

Detection of Genomic Sequences of Respiratory Syncytial Virus in Otitis Media with Effusion in Children

1992 ◽  
Vol 101 (10_suppl) ◽  
pp. 7-10 ◽  
Author(s):  
Yoshitaka Okamoto ◽  
Kazuo Kudo ◽  
Koji Shirotori ◽  
Misao Nakazawa ◽  
Eiko Ito ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Rsv Infection ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Viral Sequences

The reverse transcriptase—polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

scholarly journals Clinical impact of respiratory syncytial virus infection on children hospitalized for pertussis

2020 ◽  
Author(s):  
Ruimu Zhang ◽  
Jikui Deng
Keyword(s):  
Respiratory Syncytial Virus ◽  
Hospital Stay ◽  
Antibiotic Use ◽  
Immunofluorescence Assay ◽  
Clinical Impact ◽  
Chest Auscultation ◽  
Lactam Antibiotic ◽  
Rsv Infection ◽  
Polymerase Chain ◽  
Syncytial Virus

Abstract Background: Although Respiratory syncytial virus (RSV) is one of the common pathogens in children with pertussis and viral coinfection, the clinical impact of RSV infection on pertussis remains unclear. We compared clinical characteristics and sought differences between infants with single Bordetella pertussis (B. pertussis) infection and those with RSV coinfection.Methods: We enrolled 80 patients with pertussis who were hospitalized in Shenzhen Children’s Hospital from January 2017 to December 2019. Respiratory tract samples were tested for B. pertussis with real-time polymerase chain reaction and respiratory viruses with immunofluorescence assay. Clinical data were obtained from hospital records and collected using a structured questionnaire.Results: Thirty-seven of 80 patients had B. pertussis infection alone (pertussis group) and 43 had RSV-pertussis coinfection (coinfection group). No significant differences were found with regard to sex, body weight, preterm birth history, pertussis vaccination, symptoms, presence of pneumonia, or lymphocyte count between the 2 groups. Patients with RSV coinfection were older; received more β-lactam antibiotic treatment; had higher rates of wheezes and rales on chest auscultation, a higher rate of readmission, and a longer hospital stay.Conclusions: RSV coinfection increases β-lactam antibiotic use, readmission rate, and hospital stay in children hospitalized for pertussis. RSV infection should be suspected when wheezes or rales are present on auscultation of the chest in these patients. Early detection of RSV may avoid unnecessary antibiotic use.

scholarly journals Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

2008 ◽  
Vol 50 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Alexanda Dias Reis ◽  
Maria Cristina Domingues Fink ◽  
Clarisse Martins Machado ◽  
José de Paula Paz Jr. ◽  
Renato Reis Oliveira ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Direct Immunofluorescence ◽  
Nasopharyngeal Aspirate ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Viral Isolation ◽  
Respiratory Tract Illnesses ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Conventional Cell

A total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11.4%) were positive for RSV, considering the three techniques. PCR was the most sensitive technique, providing positive findings in 35/316 (11.1%) of the samples, followed by direct immunofluorescence (25/316, 7.9%) and viral isolation (20/315, 6.3%) (p < 0.001). A sample was positive by immunofluorescence and negative by PCR, and 11 (31.4%) were positive only by RT-PCR. We conclude that RT-PCR is more sensitive than IF and viral isolation to detect RSV in nasopharyngeal aspirate specimens in newborn and infants.

scholarly journals Application of RT-PCR for diagnosis of respiratory syncytial virus and human metapneumovirus infections in Bulgaria, 2006-7 and 2007-8

2009 ◽  
Vol 14 (23) ◽  
Author(s):  
S Pavlova ◽  
T Hadzhiolova ◽  
P Abadjieva ◽  
R Kotseva
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Diseases ◽  
National Laboratory ◽  
Human Metapneumovirus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Acute Respiratory Diseases ◽  
Polymerase Chain ◽  
Syncytial Virus

We describe here the results of respiratory syncytial virus (RSV) detection by reverse transcription polymerase chain reaction (RT-PCR) during two consecutive seasons, from December 2006 to February 2007 and from October 2007 to March 2008, performed in the National Laboratory of Influenza and Acute Respiratory Diseases, Bulgaria. A total number of 278 nasopharyngeal samples obtained from hospitalised children up to the age of five years were investigated for these two seasons. During the first season, the aetiological role of RSV was confirmed in 56 of 148 samples (37.8%) compared to 11 of 130 samples (8.5%) during the second season. Since the beginning of January 2008, RT-PCR for the detection of the recently identified human metapneumovirus (HMPV) has also been introduced in Bulgaria. This virus has been demonstrated as the aetiological agent in 13 out of 81 samples (16%) from children of the same age group. The use of RT-PCR allows the detection of a broader spectrum of viruses causing respiratory diseases, as well as better discrimination of the aetiological agents in clinically similar cases.

scholarly journals Respiratory Syncytial Virus Infection: Its Propensity for Bacterial Coinfection and Related Mortality in Elderly Adults

2020 ◽  
Vol 7 (12) ◽  
Author(s):  
Raphael Godefroy ◽  
Audrey Giraud-gatineau ◽  
Marie-Thérèse Jimeno ◽  
Sophie Edouard ◽  
Line Meddeb ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Hospital Mortality ◽  
Acute Respiratory Infection ◽  
Management Strategies ◽  
Chain Reaction ◽  
Rsv Infection ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Bacterial Coinfection ◽  
Related Mortality

Abstract Respiratory syncytial virus (RSV) is an increasingly recognized cause of acute respiratory infection (ARI) in adults. We compared the crude in-hospital mortality of patients with RSV infection alone with that of patients with RSV–bacterial coinfection. Overall, 12 144 hospitalized patients with ARI were screened for RSV detection by polymerase chain reaction between February 2014 and April 2019. In total, 701 (5.8%) had a positive RSV result, including 85 (12.1%) with bacterial coinfection. RSV–bacterial coinfection was associated with an increase in crude in-hospital mortality in patients &gt;65 years old (hazard ratio, 2.94; 95% CI, 1.30–6.60; P = .010). Optimized prevention and management strategies to reduce this burden are needed.

scholarly journals Impact of RSV Coinfection on Human Bocavirus in Children with Acute Respiratory Infections

2018 ◽  
Vol 65 (4) ◽  
pp. 342-351 ◽  
Author(s):  
Huiming Sun ◽  
Jiyang Sun ◽  
Wei Ji ◽  
Chuangli Hao ◽  
Yongdong Yan ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Clinical Features ◽  
Respiratory Infections ◽  
Human Bocavirus ◽  
Acute Respiratory Infections ◽  
Copy Numbers ◽  
Rsv Infection ◽  
Dna Copy Numbers ◽  
Syncytial Virus ◽  
Length Of Hospitalization

Abstract Objective The objective of this study was to assess epidemiological and clinical features of human bocavirus (HBoV) coinfection with other viruses. Method Children coinfected with HBoV between January 2012 and December 2014 were enrolled and retrospectively reviewed. Result A total of 984 patients were stratified into five groups: HBoV infection alone (n = 249), respiratory syncytial virus (RSV) infection alone (n = 649), HBoV coinfection with RSV (n = 28), with human rhinovirus (HRV) (n = 39) and with other virus (n = 19). Length of hospitalization was longer in HBoV coinfection with RSV group than HBoV (9.0 days vs. 7.0 days, p = 0.001), RSV (9.0 days vs. 8.0 days, p = 0.016) infection alone group. Pneumonia was more common in the HBoV coinfection with RSV group compared with the HBoV, RSV infection alone group, respectively (75.0% vs. 44.2%, 31.3%, p < 0.001). HBoV DNA copy numbers (383 000 copies/ml) were positively correlated with the length of hospitalization (r = 0.334, p < 0.001). Conclusion HBoV coinfection with RSV increases HBoV infection severity.

scholarly journals Simple Strategy for Rapid and Sensitive Detection of 2019 novel coronavirus Based on Antibody

2020 ◽  
Author(s):  
Xinjun Hu ◽  
Huiyang Deng ◽  
Yibing Shang ◽  
Mengnan Fan ◽  
Feng Yue
Keyword(s):  
Colloidal Gold ◽  
Detection Rate ◽  
Detection Sensitivity ◽  
Rt Pcr ◽  
Immunochromatographic Strip ◽  
Simple Strategy ◽  
Immunochromatographic Strip Test ◽  
Pcr Assays ◽  
Novel Coronavirus ◽  
Strip Test

Abstract Objectives: Since December 2019, acute respiratory disease due to 2019 novel coronavirus emerged in Wuhan city and rapidly spread throughout China. Real-time RT-PCR is widely deployed in diagnostic virology. However, the positive detection rates of RT-PCR are only 30% to 50%. Therefore, we propose a simple strategy for rapidly and sensitively detecting the IgM/IgG antibody against 2019-nCoV using a colloidal gold-based immunochromatographic strip test.Methods A total of 41 clinically 2019-nCoV suspected cases (23 males and 18 females) were enrolled. The sensitivity of colloidal gold-based immunochromatographic strip test and of RT-PCR were compared and evaluated. McNemar’s test was used to compare the detection rate of both assays (P<0.05).Results: The Antibody was detected in 63.4% (26/41) of blood specimens using the assay. In contrast, the 2019-nCoV was detected in 46.3% (19/41) of nasal and pharyngeal swab specimens using the RT-PCR assays. The detection rate obtained by this assay was markedly higher than that obtained by the RT-PCR assays (P=0.039)Conclusion: This detection assay exhibits a higher detection sensitivity than RT-PCR. More important, the assay shows the benefits of easy operation and setup. We believe that the sensitive and time-saving approach may be used as an auxiliary diagnostic tool for 2019-nCoV detection and virus screening and confirmation.

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