scholarly journals Growth temperature-dependent activity of glycerol dehydratase inEscherichia coliexpressing theCitrobacter freundii dharegulon

1992 ◽  
Vol 100 (1-3) ◽  
pp. 281-285 ◽  
Author(s):  
Rolf Daniel ◽  
Gerhard Gottschalk
2018 ◽  
Author(s):  
David B. Sauer ◽  
Da-Neng Wang

AbstractOptimal growth temperature is a fundamental characteristic of all living organisms. Knowledge of this temperature is central to the study the organism, the thermal stability and temperature dependent activity of its genes, and the bioprospecting of its genome for thermally adapted proteins. While high throughput sequencing methods have dramatically increased the availability of genomic information, the growth temperatures of the source organisms are often unknown. This limits the study and technological application of these species and their genomes. Here, we present a novel method for the prediction of growth temperatures of prokaryotes using only genomic sequences. By applying the reverse ecology principle that an organism’s genome includes identifiable adaptations to its native environment, we can predict a species’ optimal growth temperature with an accuracy of 4.69 °C root-mean-square error and a correlation coefficient of 0.908. The accuracy can be further improved for specific taxonomic clades or by excluding psychrophiles. This method provides a valuable tool for the rapid calculation of organism growth temperature when only the genome sequence is known.


2020 ◽  
Vol 528 (3) ◽  
pp. 528-530
Author(s):  
F. Doval ◽  
K. Chiba ◽  
R.J. McKenney ◽  
K.M. Ori-McKenney ◽  
M.D. Vershinin

1996 ◽  
Vol 79 (1) ◽  
pp. 305-309 ◽  
Author(s):  
Q. Liu ◽  
S. Derksen ◽  
W. Prost ◽  
A. Lindner ◽  
F. J. Tegude

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