Growth temperature-dependent activity of glycerol dehydratase in Escherichia coli expressing the Citrobacter freundii dha regulon

1992 ◽  
Vol 100 (1-3) ◽  
pp. 281-285 ◽  
Author(s):  
Rolf Daniel ◽  
Gerhard Gottschalk
Keyword(s):  
Escherichia Coli ◽  
Growth Temperature ◽  
Citrobacter Freundii ◽  
Temperature Dependent ◽  
Glycerol Dehydratase ◽  
Dependent Activity ◽  
Dha Regulon

scholarly journals Prediction of Optimal Growth Temperature using only Genome Derived Features

2018 ◽  
Author(s):  
David B. Sauer ◽  
Da-Neng Wang
Keyword(s):  
Growth Temperature ◽  
High Throughput Sequencing ◽  
Optimal Growth ◽  
Temperature Dependent ◽  
Optimal Growth Temperature ◽  
Rapid Calculation ◽  
Dependent Activity ◽  
Organism Growth ◽  
Novel Method ◽  
Living Organisms

AbstractOptimal growth temperature is a fundamental characteristic of all living organisms. Knowledge of this temperature is central to the study the organism, the thermal stability and temperature dependent activity of its genes, and the bioprospecting of its genome for thermally adapted proteins. While high throughput sequencing methods have dramatically increased the availability of genomic information, the growth temperatures of the source organisms are often unknown. This limits the study and technological application of these species and their genomes. Here, we present a novel method for the prediction of growth temperatures of prokaryotes using only genomic sequences. By applying the reverse ecology principle that an organism’s genome includes identifiable adaptations to its native environment, we can predict a species’ optimal growth temperature with an accuracy of 4.69 °C root-mean-square error and a correlation coefficient of 0.908. The accuracy can be further improved for specific taxonomic clades or by excluding psychrophiles. This method provides a valuable tool for the rapid calculation of organism growth temperature when only the genome sequence is known.

scholarly journals Temperature-Dependent Fermentation ofd-Sorbitol in Escherichia coli O157:H7

1999 ◽  
Vol 65 (9) ◽  
pp. 4245-4257 ◽  
Author(s):  
O. M. M. Bouvet ◽  
S. Pernoud ◽  
P. A. D. Grimont
Keyword(s):  
Escherichia Coli ◽  
Growth Temperature ◽  
Temperature Sensitive ◽  
Escherichia Coli O157 ◽  
Temperature Dependent ◽  
E Coli ◽  
Low Level

ABSTRACT The influence of growth temperature on the ability to fermentd-sorbitol was investigated in Escherichia coliO157:H7. It was found that O157:H7 strains have a temperature-sensitive sorbitol phenotype. d-Sorbitol transport and sorbitol-6-phosphate dehydrogenase activities were expressed in sorbitol-fermenting cells grown at 30°C but only at a low level at 40°C. Sorbitol-positive variants able to transportd-sorbitol were easily selected at 30°C from culture of Sor− E. coli O157:H7 strains.

scholarly journals Influence of Growth Temperature on the Development of Escherichia coli Polysaccharide K Antigens

Microbiology ◽  
1984 ◽  
Vol 130 (10) ◽  
pp. 2681-2684
Author(s):  
F. ORSKOV ◽  
V. SHARMAF ◽  
I. ORSKOV
Keyword(s):  
Escherichia Coli ◽  
Growth Temperature ◽  
Polysaccharide K

scholarly journals Characterization of the 6'-N-aminoglycoside acetyltransferase gene aac(6')-Im [corrected] associated with a sulI-type integron.

1997 ◽  
Vol 41 (2) ◽  
pp. 314-318 ◽  
Author(s):  
E Hannecart-Pokorni ◽  
F Depuydt ◽  
L de wit ◽  
E van Bossuyt ◽  
J Content ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Resistance Gene ◽  
Citrobacter Freundii ◽  
Gram Negative ◽  
Gene Environment ◽  
Insert Region ◽  
Klebsiella Spp

The amikacin resistance gene aac(6')-Im [corrected] from Citrobacter freundii Cf155 encoding an aminoglycoside 6'-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 521 bp located down-stream from the 5' conserved segment of an integron. The sequence of this aac(6')-Im [corrected] gene corresponded to a protein of 173 amino acids which possessed 64.2% identity in a 165-amino-acid overlap with the aac(6')-Ia gene product (F.C. Tenover, D. Filpula, K.L. Phillips, and J. J. Plorde, J. Bacteriol. 170:471-473, 1988). By using PCR, the aac(6')-Im [corrected] gene could be detected in 8 of 86 gram-negative clinical isolates from two Belgian hospitals, including isolates of Citrobacter, Klebsiella spp., and Escherichia coli. PCR mapping of the aac(6')-Im [corrected] gene environment in these isolates indicated that the gene was located within a sulI-type integron; the insert region is 1,700 bases long and includes two genes cassettes, the second being ant (3")-Ib.

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