scholarly journals Identification ofNaegleria fowleriand otherNaegleriaspp. (free-living amoebae) using cellulose acetate membrane electrophoresis of glucose phosphate isomerase

1995 ◽  
Vol 133 (3) ◽  
pp. 219-223 ◽  
Author(s):  
Simon Kilvington
Keyword(s):  
Cellulose Acetate ◽  
Cellulose Acetate Membrane ◽  
Glucose Phosphate Isomerase ◽  
Free Living ◽  
Glucose Phosphate

scholarly journals A new allele of the Gpi-1t temporal gene that regulates the expression of glucose phosphate isomerase in mouse oocytes

1984 ◽  
Vol 44 (2) ◽  
pp. 169-181 ◽  
Author(s):  
John D. West ◽  
Graham Fisher
Keyword(s):  
Cellulose Acetate ◽  
Structural Gene ◽  
The Other ◽  
Glucose Phosphate Isomerase ◽  
Cellulose Acetate Electrophoresis ◽  
Mouse Oocytes ◽  
Glucose Phosphate ◽  
Somatic Tissues ◽  
Developmental Programme

The dimeric enzyme glucose phosphate isomerase (GPI-1) is regulated in oocytes by a cis-acting temporal gene (Gpi-1t) that maps close to the structural gene (Gpi-1s). Quantitative cellulose acetate electrophoresis of GPI-1 allozymes from unfertilized eggs produced by various Gpi-1sa / Gpi-1sb heterozygous females revealed a new Gpi-1t allele that we have designated Gpi-1tc. This allele is present in 101/H mice and a partially congenie stock that carries the Gpi-lsa gene derived from the AKR strain. We have confirmed that Gpi-1tc is closely linked to Gpi-1s and that it is cis-acting. It produces higher levels of GPI-1 in unfertilized eggs than the other two Gpi-lt alleles that are known (Gpi-1ta and Gpi-1tb) but has no effect on GPI-1 in somatic tissues or spermatozoa. This new Gpi-1t allele represents a third developmental programme for GPI-1 expression in oocytes.

scholarly journals The transition from oocyte-coded to embryo-coded glucose phosphate isomerase in the early mouse embryo

Development ◽  
1983 ◽  
Vol 78 (1) ◽  
pp. 127-140
Author(s):  
John D. West ◽  
J. F. Green
Keyword(s):  
Cellulose Acetate ◽  
Mouse Embryo ◽  
Mouse Embryos ◽  
Glucose Phosphate Isomerase ◽  
Cellulose Acetate Electrophoresis ◽  
Early Mouse Embryo ◽  
Glucose Phosphate ◽  
Early Mouse

The proportions of glucose phosphate isomerase (GPI-1) allozymes produced by early (Gpi-1sa/Gpi-1sb ♀ × Gpi-1sc/Gpi-1sc ♂)F1 mouse embryos were analysed by quantitative cellulose acetate electrophoresis. Technical controls showed that this system is extremely sensitive, quantitatively reproducible and quite accurate. Genetic controls established that the Gpi-1sa/Gpi-1sb mothers were homozygous for the Gpi-1tb temporal allele, that produces relatively high GPI-1 activity in the oocyte. The oocyte-coded enzyme lasted until about 5½ days post coitum (p.c.) or shortly thereafter. The maternally derived, embryonic Gpi-1s allele was expressed no earlier than the paternally derived allele. This was first expressed between 2½ and 3½ days p.c. In this cross, most of the transition from oocyte-coded to embryo-coded GPI-1 occurred between 2½ and 3½ days p.c.

Effect of the structure of cellulose acetate membranes on their permeability, electrical conductivity and rejection

1990 ◽  
Vol 55 (12) ◽  
pp. 2933-2939 ◽  
Author(s):  
Hans-Hartmut Schwarz ◽  
Vlastimil Kůdela ◽  
Klaus Richau
Keyword(s):  
Electrical Conductivity ◽  
Electrical Properties ◽  
Cellulose Acetate ◽  
Reverse Osmosis ◽  
Water Flux ◽  
Cellulose Acetate Membrane ◽  
Structure Parameters ◽  
Dc Electrical Conductivity ◽  
Cellulose Acetate Membranes

Ultrafiltration cellulose acetate membrane can be transformed by annealing into reverse osmosis membranes (RO type). Annealing brings about changes in structural properties of the membranes, accompanied by changes in their permeability behaviour and electrical properties. Correlations between structure parameters and electrochemical properties are shown for the temperature range 20-90 °C. Relations have been derived which explain the role played by the dc electrical conductivity in the characterization of rejection ability of the membranes in the reverse osmosis, i.e. rRO = (1 + exp (A-B))-1, where exp A and exp B are statistically significant correlation functions of electrical conductivity and salt permeation, or of electrical conductivity and water flux through the membrane, respectively.

Alginate fouling reduction of functionalized carbon nanotube blended cellulose acetate membrane in forward osmosis

Chemosphere ◽  
2015 ◽  
Vol 136 ◽  
pp. 204-210 ◽  
Author(s):  
Hyeon-gyu Choi ◽  
Moon Son ◽  
SangHyeon Yoon ◽  
Evrim Celik ◽  
Seoktae Kang ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Cellulose Acetate ◽  
Forward Osmosis ◽  
Cellulose Acetate Membrane ◽  
Functionalized Carbon Nanotube ◽  
Fouling Reduction

Characterization of the 5′ end of the gene for human glucose phosphate isomerase (GPI)

Genomics ◽  
1990 ◽  
Vol 7 (4) ◽  
pp. 638-643 ◽  
Author(s):  
James I.H. Walker ◽  
Pelin Faik ◽  
Michael J. Morgan
Keyword(s):  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

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