A new allele of the Gpi-1t temporal gene that regulates the expression of glucose phosphate isomerase in mouse oocytes

The dimeric enzyme glucose phosphate isomerase (GPI-1) is regulated in oocytes by a cis-acting temporal gene (Gpi-1t) that maps close to the structural gene (Gpi-1s). Quantitative cellulose acetate electrophoresis of GPI-1 allozymes from unfertilized eggs produced by various Gpi-1sa / Gpi-1sb heterozygous females revealed a new Gpi-1t allele that we have designated Gpi-1tc. This allele is present in 101/H mice and a partially congenie stock that carries the Gpi-lsa gene derived from the AKR strain. We have confirmed that Gpi-1tc is closely linked to Gpi-1s and that it is cis-acting. It produces higher levels of GPI-1 in unfertilized eggs than the other two Gpi-lt alleles that are known (Gpi-1ta and Gpi-1tb) but has no effect on GPI-1 in somatic tissues or spermatozoa. This new Gpi-1t allele represents a third developmental programme for GPI-1 expression in oocytes.

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The transition from oocyte-coded to embryo-coded glucose phosphate isomerase in the early mouse embryo

Development ◽

10.1242/dev.78.1.127 ◽

1983 ◽

Vol 78 (1) ◽

pp. 127-140

Author(s):

John D. West ◽

J. F. Green

Keyword(s):

Cellulose Acetate ◽

Mouse Embryo ◽

Mouse Embryos ◽

Glucose Phosphate Isomerase ◽

Cellulose Acetate Electrophoresis ◽

Early Mouse Embryo ◽

Glucose Phosphate ◽

Early Mouse

The proportions of glucose phosphate isomerase (GPI-1) allozymes produced by early (Gpi-1sa/Gpi-1sb ♀ × Gpi-1sc/Gpi-1sc ♂)F1 mouse embryos were analysed by quantitative cellulose acetate electrophoresis. Technical controls showed that this system is extremely sensitive, quantitatively reproducible and quite accurate. Genetic controls established that the Gpi-1sa/Gpi-1sb mothers were homozygous for the Gpi-1tb temporal allele, that produces relatively high GPI-1 activity in the oocyte. The oocyte-coded enzyme lasted until about 5½ days post coitum (p.c.) or shortly thereafter. The maternally derived, embryonic Gpi-1s allele was expressed no earlier than the paternally derived allele. This was first expressed between 2½ and 3½ days p.c. In this cross, most of the transition from oocyte-coded to embryo-coded GPI-1 occurred between 2½ and 3½ days p.c.

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Intercellular Glycosaminoglycans in Human Cancer

Tumori Journal ◽

10.1177/030089167906500603 ◽

1979 ◽

Vol 65 (6) ◽

pp. 677-686 ◽

Cited By ~ 1

Author(s):

Maria Cristina Cella ◽

Gabriella Fibbi ◽

Cristina Cantini ◽

Zita Del Panta ◽

Simonetta Vannucchi ◽

...

Keyword(s):

Cellulose Acetate ◽

Molecular Species ◽

Normal Tissue ◽

Human Cancer ◽

Electrophoretic Pattern ◽

Neoplastic Transformation ◽

Relative Increase ◽

The Other ◽

Cellulose Acetate Electrophoresis ◽

Consistent Feature

Intercellular glycosaminoglycans (GAGs) from various tissues were analyzed by cellulose acetate electrophoresis and enzymatic treatment with specific mucopolysaccharidases. Each tissue exhibits a particular composition of sulfated and unsulfated molecular species. Invariably, malignant human neoplasias and their metastases show striking variations in the electrophoretic pattern typical of the corresponding normal tissue. An absolute or relative increase in surface ChS A/C and HA seems to be a consistent feature of neoplastic transformation. On the other hand, the GAGs composition of benign noninfiltrative tumors does not vary greatly with respect to the original normal tissue.

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Identification ofNaegleria fowleriand otherNaegleriaspp. (free-living amoebae) using cellulose acetate membrane electrophoresis of glucose phosphate isomerase

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.1995.tb07888.x ◽

1995 ◽

Vol 133 (3) ◽

pp. 219-223 ◽

Cited By ~ 2

Author(s):

Simon Kilvington

Keyword(s):

Cellulose Acetate ◽

Cellulose Acetate Membrane ◽

Glucose Phosphate Isomerase ◽

Free Living ◽

Glucose Phosphate

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Strain specific immunity to Plasmodium berghei: a new genetic marker

Parasitology ◽

10.1017/s0031182000046254 ◽

1973 ◽

Vol 67 (1) ◽

pp. 17-27 ◽

Cited By ~ 15

Author(s):

A. I. Oxbrow

Keyword(s):

Plasmodium Berghei ◽

Prolonged Exposure ◽

Genetic Recombination ◽

The Other ◽

Glucose Phosphate Isomerase ◽

Glucose Phosphate ◽

Frequent Event ◽

Cross Immunity ◽

The Cross ◽

The Difference

Mice immunized by single pyrimethamine terminated infections of Plasmodium berghei berghei, strain NK65 or the Nigerian P. berghei-like parasite, N67, were immune to challenge by P.b. berghei, P.b. yoelii strain 17X, P.b. killicki strain 194ZZ and N67. Mice similarly immunized against P.b. yoelii were only immune or N67 resulted in infections comparable to those found in normal mice. Mice immunized against P.b. killicki were similar except that they also possessed some protection against challenge by N67. Evidence was obtained which indicated that the difference between P.b. yoelii and N67 was not associated with the virulence of the parasites. Prolonged exposure to P.b. yoelii infection slightly increased homologous and heterologous immunity. Two lines of parasites were crossed; one of these was derived from P.b. yoelii and the other from N67. They differed in pyrimethamine sensitivity, glucose phosphate isomerase type and the ability to survive in mice immunized against P.b. yoelii. Ten lines of parasites showing the P.b. yoelii characters of pyrimethamine resistance and GPI-1 were isolated in drug treated mice immune to P.b. yoelii challenge. Twenty-one clones were isolated without drug or antibody selection from the parasites resulting from the cross between P.b. yoelii and N67. Sixteen of these clones were classified according to the markers which they possessed and six were found to be recombinant in character. The experiments showed that genetic recombination occurred as a frequent event in P. berghei and involved factors which control the cross-immunity produced following infection with these parasites.

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Genetic regulation of glucose phosphate isomerase in mouse oocytes

Nature ◽

10.1038/276267a0 ◽

1978 ◽

Vol 276 (5685) ◽

pp. 267-269 ◽

Cited By ~ 21

Author(s):

A. C. PETERSON ◽

G. G. WONG

Keyword(s):

Genetic Regulation ◽

Glucose Phosphate Isomerase ◽

Mouse Oocytes ◽

Glucose Phosphate

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GPI expression in female germ cells of the mouse

Genetics Research ◽

10.1017/s0016672300020309 ◽

1981 ◽

Vol 37 (3) ◽

pp. 303-309 ◽

Cited By ~ 17

Author(s):

Anne McLaren ◽

Mia Buehr

Keyword(s):

Structural Gene ◽

Germ Cells ◽

Gene Complex ◽

Glucose Phosphate Isomerase ◽

Specific Expression ◽

Isomerase Activity ◽

Glucose Phosphate ◽

Form Part ◽

Genetically Determined

SUMMARYThe genetically determined oocyte-specific expression of glucose-phosphate isomerase activity in the mouse is first apparent at 6 to 7 days after birth, and occurs in XO as well as in XX oocytes. The regulator locus that controls oocyte-specific expression shows the same linkage relations as the structural gene, suggesting that both form part of a Gpi-1 gene complex.

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Activation of the lac Repressor in the Transgenic Mouse

Genetics ◽

10.1093/genetics/147.1.297 ◽

1997 ◽

Vol 147 (1) ◽

pp. 297-304

Author(s):

Heidi Scrable ◽

Peter J Stambrook

Keyword(s):

Copy Number ◽

Methylation Status ◽

Lac Repressor ◽

The Other ◽

Lac Operon ◽

Normal Process ◽

Divergent Sequence ◽

Somatic Tissues ◽

New Material ◽

Bacterial Sequence

Abstract We have introduced sequences encoding the lac repressor of Escherichia coli into the genome of the mouse. One sequence was derived from the bacterial lac operon and the other was created by reencoding the amino acid sequence of lacI with mammalian codons. Both versions are driven by an identical promoter fragment derived from the human β-actin locus and were microinjected into genetically identical pronuclear stage embryos. All transgenes utilizing the bacterial coding sequence were transcriptionally silent in all somatic tissues tested. The sequence re-encoded with mammalian codons was transcriptionally active at all transgene loci and expressed ubiquitously. Using methylation-sensitive enzymes, we have determined the methylation status of lac repressor transgenes encoded by either the bacterial or mammalian sequence. The highly divergent bacterial sequence was hypermethylated at all transgene loci, while the mammalian sequence was only hypermethylated at a high copy number locus. This may reflect a normal process that protects the genome from acquiring new material that has an abnormally divergent sequence or structure.

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Induction of DNA replication in germinal vesicles and in nuclei formed in maturing mouse oocytes by 6-DMAP treatment

Zygote ◽

10.1017/s0967199400003646 ◽

1997 ◽

Vol 5 (3) ◽

pp. 213-217 ◽

Cited By ~ 3

Author(s):

J. Fulka ◽

N.L. First ◽

C. Lee ◽

J. Fulka ◽

R.M. Moor

Keyword(s):

Dna Replication ◽

Dna Synthesis ◽

Germinal Vesicle ◽

The Other ◽

Immature Oocytes ◽

Mouse Oocytes ◽

Germinal Vesicles ◽

Immature Mouse ◽

Condensed Dna ◽

Metaphase Ii Oocytes

SummaryImmature mouse oocytes (germinal vesicle stage, GV), oocytes at different stages during maturation (prometaphase to anaphase I) and matured oocytes (metaphase II arrested) were cultured in 6-dimethylaminopurine (6-DMAP)-supplemented medium also containing bromodeoxyuridine for the assessment of DNA replication in these cells. Immature oocytes remained arrested at the GV stage and DNA replication was never detected in them. On the other hand, oocytes at the prometaphase to anaphase-telophase I stages responded to 6-DMAP treatment by forming nuclei which synthesised DNA. Mature (metaphase II) oocytes did not respond to 6-DMAP and their chromatin remained condensed. DNA synthesis could even be induced in GV-staged oocytes, but only when they were fused to freshly activated oocytes and incubated in 6-DMAP-supplemented medium.

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