SopB effector protein of Salmonella Typhimurium is translocated in mesenteric lymph nodes during murine salmonellosis

During the period July, 1980 to June, 1981, 618 samples consisting of mesenteric lymph nodes, spleens and feces, collected from 307 sheep and goats slaughtered in the Riyadh Public Abattoir, were examined for salmonellae. Salmonellae were recovered from 14.7% of 307 lymph nodes, 4.7% of 192 feces and from 0.8% of 119 spleens. Among the 23 serotypes recovered, the most common was Salmonella typhimurium followed by S. newport, S. havana, S. bovismorbificans, S. reading, S. braenderup, S. eastbourne, and S. poona. Other less common serotypes were also encountered. Lymph nodes and feces from slaughtered animals may be a source for contamination of the red meat and other edible parts of the carcase with salmonellae. Consumption of contaminated meat or meat products either raw or undercooked may cause Salmonella food poisoning in man.

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AvrA effector protein of Salmonella enterica serovar Enteritidis is expressed and translocated in mesenteric lymph nodes at late stages of infection in mice

Microbiology ◽

10.1099/mic.0.077115-0 ◽

2014 ◽

Vol 160 (6) ◽

pp. 1191-1199 ◽

Cited By ~ 9

Author(s):

Mónica N. Giacomodonato ◽

Mariángeles Noto Llana ◽

María del Rosario Aya Castañeda ◽

Fernanda R. Buzzola ◽

Sebastián H. Sarnacki ◽

...

Keyword(s):

Lymph Nodes ◽

Culture Conditions ◽

Effector Protein ◽

Post Inoculation ◽

Mesenteric Lymph Nodes ◽

Salmonella Enterica Serovar Enteritidis ◽

Major Health Problem ◽

Mesenteric Lymph

Salmonellosis is a major health problem worldwide. Salmonella enterica serovar Enteritidis (S. Enteritidis) has been a primary cause of Salmonella outbreaks in many countries. AvrA is an SPI-1 effector protein involved in the enteritis pathway, with critical roles in inhibiting inflammation and apoptosis. In this work, we constructed an AvrA-FLAG-tagged strain of S. Enteritidis to analyse the expression profile of AvrA in vitro, in cell culture and in vivo. AvrA expression and secretion were observed in vitro under culture conditions that mimicked intestinal and intracellular environments. In agreement, bacteria isolated from infected cell monolayers expressed and translocated AvrA for at least 24 h post-inoculation. For in vivo experiments, BALB/c mice were inoculated by the natural route of infection with the AvrA-FLAG strain. Infecting bacteria and infected cells were recovered from mesenteric lymph nodes (MLN). Our results showed that AvrA continues to be synthesized in vivo up to day 8 post-inoculation. Moreover, AvrA translocation was detected in the cytosol of cells isolated from MLN 8 days after infection. Interestingly, we observed that AvrA is secreted by both type three secretion system (T3SS)-1 and T3SS-2. In summary, these findings indicate that AvrA expression is not constrained to the initial host–bacteria encounter in the intestinal environment as defined previously. The AvrA effector may participate also in systemic S. Enteritidis infection.

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Pathogen load and expression of immune-related genes in mesenteric lymph-nodes of pigs experimentally infected with Salmonella typhimurium

New Biotechnology ◽

10.1016/j.nbt.2010.01.077 ◽

2010 ◽

Vol 27 ◽

pp. S49

Author(s):

R.P. Martins ◽

M. Collado-Romero ◽

C. Arce ◽

J.J. Garrido

Keyword(s):

Lymph Nodes ◽

Salmonella Typhimurium ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph ◽

Pathogen Load ◽

Immune Related Genes

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Faculty Opinions recommendation of Salmonella typhimurium persists within macrophages in the mesenteric lymph nodes of chronically infected Nramp1+/+ mice and can be reactivated by IFNgamma neutralization.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1017249.202372 ◽

2004 ◽

Author(s):

Francisco Garcia-del Portillo

Keyword(s):

Lymph Nodes ◽

Salmonella Typhimurium ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph

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ULTRASTRUCTURAL CHANGES OF THE MESENTRIC LYMPH NODES OF CALVES EXPERIMENTALLY INFECTED WITH SALMONELLA TYPHIMURIUM

The Iraqi Journal of Veterinary Medicine ◽

10.30539/ijvm.v21i1.1247 ◽

2022 ◽

Vol 21 (1) ◽

pp. 72-84

Author(s):

Yass, A.W. ◽

Habasha, F.G. F.G. ◽

Al-Sammarai, S.

Keyword(s):

Lymph Nodes ◽

Salmonella Typhimurium ◽

Host Cells ◽

Ultrastructural Changes ◽

Mesenteric Lymph Nodes ◽

Ultrastructural Alteration ◽

Mesenteric Lymph ◽

Group I ◽

Experimentally Induced

A systemic study was done on the pathogenesis of experimentally induced Salmonella typhimurium infection in calves. The present investigation was carried out on sixteen normal colostrum fed friesian calves, ranging in age from 3 to 6 weeks. The calves were divided into two equal groups. Group I inoculated orally with (1.5 x 10'') Salmonella typhimurium and group IA served as control. • The early ultrastructural alteration in the mesenteric lymph nodes was the presence of many free Salmonella in localized vacuoles. The interaction between the host cells and phagocytized Salmonella was also observed.

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