ULTRASTRUCTURAL CHANGES OF THE MESENTRIC LYMPH NODES OF CALVES EXPERIMENTALLY INFECTED WITH  SALMONELLA TYPHIMURIUM

A systemic study was done on the pathogenesis of experimentally induced Salmonella typhimurium infection in calves. The present investigation was carried out on sixteen normal colostrum fed friesian calves, ranging in age from 3 to 6 weeks. The calves were divided into two equal groups. Group I inoculated orally with (1.5 x 10'') Salmonella typhimurium and group IA served as control. • The early ultrastructural alteration in the mesenteric lymph nodes was the presence of many free Salmonella in localized vacuoles. The interaction between the host cells and phagocytized Salmonella was also observed.

Download Full-text

Prior immunity to Trichinella spiralis prevents (re)occurrence of an explicit stress response in intestines but not in mesenteric lymph nodes, heart and lungs from reinfected rats

Parasitology ◽

10.1017/s003118209900671x ◽

2000 ◽

Vol 121 (5) ◽

pp. 565-573 ◽

Cited By ~ 1

Author(s):

J. PÉREZ-SERRANO ◽

J. MARTÍNEZ ◽

P. REGAL ◽

W. E. BERNADINA ◽

F. RODRÍGUEZ-CAABEIRO

Keyword(s):

Heat Shock ◽

Lymph Nodes ◽

Rat Model ◽

Trichinella Spiralis ◽

Host Cells ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph ◽

Muscle Larvae ◽

Shock Response ◽

Heat Shock Responses

We recently showed that, in our Trichinella spiralis rat model, first exposure, but not re-exposure to infective-stage larvae evoked heat shock responses in 4 test organs. Our work, however, failed to implicate either early complete clearance of challenge muscle larvae (ML), or rapid elimination of newborn larvae (NBL) in the phenomenon noted in reinfected rats. This study clarifies that issue using 2 established facts in T. spiralis biology and anti-T. spiralis immunology. That is, adult worms injure gut cells and immune destruction of NBL requires release of material also toxic to host cells. To approach the above problem we analysed relevant and irrelevant rat organs for increased heat shock protein (HSP) production at 1, 7, 14, 20 and 27 p.i. during first and second infections. Organs examined were intestines, mesenteric lymph nodes (MLN), heart and lungs. Using densitometric analyses of immunoblots, increased HSP expression was detected on day 7 in intestines from both primary and secondary-infected rats albeit that the change in the latter was just short of significant. Interestingly, MLN only exhibited increased HSP levels in the reinfected rat model with increased HSP levels persisting for 1 week. A lasting shock response was detected in reinfected rats; in contrast, first exposure resulted in shock responses being evident in lungs at either day 7 or day 14, only. These findings suggest that (i) in immune rats, a few challenge ML develop into adults, produce NBLwhich are trapped within MLN, and (ii) that anti-T. spiralis and/or anti-NBL immunity is associated with an, as yet, uncomprehended stress to host's heart tissues.

Download Full-text

Identification of Attenuated Yersinia pseudotuberculosis Strains and Characterization of an Orogastric Infection in BALB/c Mice on Day 5 Postinfection by Signature-Tagged Mutagenesis

Infection and Immunity ◽

10.1128/iai.67.5.2779-2787.2001 ◽

2001 ◽

Vol 69 (5) ◽

pp. 2779-2787 ◽

Cited By ~ 90

Author(s):

Joan Mecsas ◽

Inna Bilis ◽

Stanley Falkow

Keyword(s):

Gastrointestinal Tract ◽

Lymph Nodes ◽

Yersinia Pseudotuberculosis ◽

Proximal Colon ◽

Host Cells ◽

Wild Type ◽

Mesenteric Lymph Nodes ◽

Host Animal ◽

Gene Encoding ◽

Mesenteric Lymph

ABSTRACT Yersinia pseudotuberculosis localizes to the distal ileum, cecum, and proximal colon of the gastrointestinal tract after oral infection. Using signature-tagged mutagenesis, we isolated 13Y. pseudotuberculosis mutants that failed to survive in the cecum of mice after orogastric inoculation. Twelve of these mutants were also attenuated for replication in the spleen after intraperitoneal infection, whereas one strain, mutated the gene encoding invasin, replicated as well as wild-type bacteria in the spleen. Several mutations were in operons encoding components of the type III secretion system, including components involved in translocating Yop proteins into host cells. This indicates that one or more Yops may be necessary for survival in the gastrointestinal tract. Three mutants were defective in O-antigen biosynthesis; these mutants were also unable to invade epithelial cells as efficiently as wild-typeY. pseudotuberculosis. Several other mutations were in genes that had not previously been associated with growth in a host, including cls, ksgA, and sufl. In addition, using Y. pseudotuberculosis strains marked with signature tags, we counted the number of different bacterial clones that were present in the cecum, mesenteric lymph nodes, and spleen 5 days postinfection. We find barriers in the host animal that limit the number of bacteria that succeed in reaching and/or replicating in the mesenteric lymph nodes and spleen after breaching the gut mucosa.

Download Full-text

Faculty Opinions recommendation of Salmonella typhimurium persists within macrophages in the mesenteric lymph nodes of chronically infected Nramp1+/+ mice and can be reactivated by IFNgamma neutralization.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1017249.202364 ◽

2004 ◽

Author(s):

Virginia L Miller

Keyword(s):

Lymph Nodes ◽

Salmonella Typhimurium ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph

Download Full-text

Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection

Journal of Proteomics ◽

10.1016/j.jprot.2012.03.045 ◽

2012 ◽

Vol 75 (14) ◽

pp. 4457-4470 ◽

Cited By ~ 15

Author(s):

Rodrigo Prado Martins ◽

Melania Collado-Romero ◽

Montserrat Martínez-Gomáriz ◽

Ana Carvajal ◽

Concepción Gil ◽

...

Keyword(s):

Lymph Nodes ◽

Salmonella Typhimurium ◽

Proteomic Analysis ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph

Download Full-text

Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection

Veterinary Research ◽

10.1186/s13567-018-0506-1 ◽

2018 ◽

Vol 49 (1) ◽

Cited By ~ 6

Author(s):

Juber Herrera-Uribe ◽

Sara Zaldívar-López ◽

Carmen Aguilar ◽

Cristina Luque ◽

Rocío Bautista ◽

...

Keyword(s):

Lymph Nodes ◽

Salmonella Typhimurium ◽

Regulatory Role ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph

Download Full-text

Incidence of Salmonellae in Lymph Nodes, Spleens and Feces of Sheep and Goats Slaughtered in the Riyadh Public Abattoir

Journal of Food Protection ◽

10.4315/0362-028x-45.14.1314 ◽

1982 ◽

Vol 45 (14) ◽

pp. 1314-1317 ◽

Cited By ~ 13

Author(s):

NASSIM H. NABBUT ◽

HABEEB M. AL-NAKHLI

Keyword(s):

Lymph Nodes ◽

Salmonella Typhimurium ◽

Food Poisoning ◽

Meat Products ◽

Red Meat ◽

Mesenteric Lymph Nodes ◽

Sheep And Goats ◽

Mesenteric Lymph ◽

Common Serotypes ◽

Edible Parts

During the period July, 1980 to June, 1981, 618 samples consisting of mesenteric lymph nodes, spleens and feces, collected from 307 sheep and goats slaughtered in the Riyadh Public Abattoir, were examined for salmonellae. Salmonellae were recovered from 14.7% of 307 lymph nodes, 4.7% of 192 feces and from 0.8% of 119 spleens. Among the 23 serotypes recovered, the most common was Salmonella typhimurium followed by S. newport, S. havana, S. bovismorbificans, S. reading, S. braenderup, S. eastbourne, and S. poona. Other less common serotypes were also encountered. Lymph nodes and feces from slaughtered animals may be a source for contamination of the red meat and other edible parts of the carcase with salmonellae. Consumption of contaminated meat or meat products either raw or undercooked may cause Salmonella food poisoning in man.

Download Full-text