Rapid detection of vip1-type genes from Bacillus cereus and characterization of a novel vip binary toxin gene

Abstract Bacillus cereus is considered to be an important food poisoning agent causing diarrhea and vomiting. In this study, the occurrence of B. cereus bacteriophages in Thai fermented soybean products (Thua Nao) was studied using five B. cereus sensu lato indicator strains (four B. cereus strains and one B. thuringiensis strain). In a total of 26 Thua Nao samples, there were only two bacteriophages namely BaceFT01 and BaceCM02 exhibiting lytic activity against B. cereus. Morphological analysis revealed that these two bacteriophages belonged to the Myoviridae. Both phages were specific to B. cereus and not able to lyse other tested bacteria including B. licheniformis and B. subtilis. The two phages were able to survive in a pH range between 5 and 12. However, both phages were inactive either by treatment of 50°C for 2 h or exposure of UV for 2 h. It should be noted that both phages were chloroform-insensitive, however. This is the first report describing the presence of bacteriophages in Thua Nao products. The characterization of these two phages is expected to be useful in the food industry for an alternative strategy including the potential use of the phages as a biocontrol candidate against foodborne pathogenic bacteria.

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Characterization of two newly isolated bacteriophages PW2 and PW4 and derived endolysins with lysis activity against Bacillus cereus group strains

Virus Research ◽

10.1016/j.virusres.2021.198489 ◽

2021 ◽

pp. 198489

Author(s):

Xiaofu Wan ◽

Peiling Geng ◽

Jiahui Sun ◽

Zhiming Yuan ◽

Xiaomin Hu

Keyword(s):

Bacillus Cereus ◽

Bacillus Cereus Group ◽

Lysis Activity

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Characterization of an iron-regulated promoter involved in desferrioxamine B synthesis in Streptomyces pilosus: repressor-binding site and homology to the diphtheria toxin gene promoter.

Journal of Bacteriology ◽

10.1128/jb.175.11.3295-3302.1993 ◽

1993 ◽

Vol 175 (11) ◽

pp. 3295-3302 ◽

Cited By ~ 38

Author(s):

K Günter ◽

C Toupet ◽

T Schupp

Keyword(s):

Binding Site ◽

Diphtheria Toxin ◽

Gene Promoter ◽

Toxin Gene ◽

Desferrioxamine B ◽

Repressor Binding Site

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Analysis of enterotoxigenic Bacillus cereus strains from dried foods using whole genome sequencing, multi-locus sequence analysis and toxin gene prevalence and distribution using endpoint PCR analysis

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2018.06.016 ◽

2018 ◽

Vol 284 ◽

pp. 31-39 ◽

Cited By ~ 9

Author(s):

Laurenda Carter ◽

Hannah R. Chase ◽

Charles M. Gieseker ◽

Nicholas R. Hasbrouck ◽

Cynthia B. Stine ◽

...

Keyword(s):

Sequence Analysis ◽

Bacillus Cereus ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Pcr Analysis ◽

Toxin Gene ◽

Whole Genome ◽

Dried Foods

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Characterization of germination and outgrowth of sorbic acid-stressed Bacillus cereus ATCC 14579 spores: Phenotype and transcriptome analysis

Food Microbiology ◽

10.1016/j.fm.2010.04.005 ◽

2011 ◽

Vol 28 (2) ◽

pp. 275-283 ◽

Cited By ~ 35

Author(s):

C.C.J. van Melis ◽

M.N. Nierop Groot ◽

M.H. Tempelaars ◽

R. Moezelaar ◽

T. Abee

Keyword(s):

Bacillus Cereus ◽

Transcriptome Analysis ◽

Sorbic Acid

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Biosynthesis and Characterization of Poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) from Bacillus cereus S10

Journal of Polymers and the Environment ◽

10.1007/s10924-012-0457-y ◽

2012 ◽

Vol 20 (3) ◽

pp. 865-871 ◽

Cited By ~ 14

Author(s):

Farha Masood ◽

Fariha Hasan ◽

Safia Ahmed ◽

P. Chen ◽

Abdul Hameed

Keyword(s):

Bacillus Cereus

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Biochemical characterization of a cortexless mutant of a variant of Bacillus cereus

Canadian Journal of Microbiology ◽

10.1139/m76-147 ◽

1976 ◽

Vol 22 (7) ◽

pp. 1007-1012 ◽

Cited By ~ 2

Author(s):

Susanne M. Pearce

Keyword(s):

Cell Wall ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Bacillus Cereus ◽

Biochemical Characterization ◽

Diaminopimelic Acid ◽

Peptidoglycan Synthesis ◽

Biochemical Lesion ◽

Do So

Previous studies on this cortexless mutant of Bacillus cereus var. alesti indicated that the forespore membrane was the site of the biochemical lesion. This hypothesis is supported by the results presented here: fatty acid composition of sporulating cells of the mutant is altered, while in vegetative cells it is comparable to the parent; soluble precursors of peptidoglycan synthesis are accumulated in the mutant, at the time of cortex formation; homogenates of the mutant prepared at the time of cortex formation are unable to incorporate tritiated diaminopimelic acid into peptidoglycan, while homogenates of cells forming germ cell wall do so to an extent comparable to that of the parent; lipid-linked intermediates are formed by the mutant as in the parent. Apparently the mutant is unable either to transfer disaccharide penta-peptide units from the carrier lipid to the growing peptidoglycan acceptor, or to transport lipid-linked intermediates across the forespore membrane.

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