Semiquantitative Determination of Fc and C3 Receptors on Human Lymphocytes by Isotope-Labelled Marker Cells

2009 ◽  
Vol 19 (4) ◽  
pp. 367-375 ◽  
Author(s):  
Viggo Jønsson ◽  
Bjarne Egelund Christensen
Keyword(s):  
Human Lymphocytes ◽  
Semiquantitative Determination ◽  
C3 Receptors

Comparison of several methods for semiquantitative determination of urinary protein.

1977 ◽  
Vol 23 (5) ◽  
pp. 876-879 ◽  
Author(s):  
W L Gyure
Keyword(s):  
Serum Albumin ◽  
Salt Concentration ◽  
Urine Samples ◽  
Urine Protein ◽  
Sulfosalicylic Acid ◽  
Green Method ◽  
Semiquantitative Determination ◽  
Acid Method ◽  
Bromcresol Green

Abstract Two types of urine protein dipsticks and the sulfosalicylic acid method were compared for their accuracy and specificity, with use of urine samples supplemented with various proteins. Dipsticks yield accurate results when the protein under consideration is restricted to albumin; the sulfosalicylic acid method accurately determines many kinds of proteins in addition to albumin. Detergents affect each of the methods, but changes in salt concentration only affect results by dipstick procedures. Dipsticks, which are based on the protein-error principle for indicators, are subject to some of the conditions that apply to the bromcresol green method for serum albumin determination.

Simple semiquantitative determination of trace metal ions by use of reagent gel columns—II

Talanta ◽  
1976 ◽  
Vol 23 (3) ◽  
pp. 244-246 ◽  
Author(s):  
Yong Keun Lee ◽  
Kyu Ja Whang ◽  
Keihei Ueno
Keyword(s):  
Trace Metal ◽  
Metal Ions ◽  
Trace Metal Ions ◽  
Semiquantitative Determination

Semiquantitative determination of alkaloids by circular thin-layer chromatography

1969 ◽  
Vol 57 (3) ◽  
pp. 449-455 ◽  
Author(s):  
Manzur-ul-Haque Hashmi ◽  
Shahnaz Parveen ◽  
N. A. Chughtai
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Semiquantitative Determination ◽  
Layer Chromatography

Application of the Solution of the Inverse Light Scattering Problem for Characterization of Mononuclear Cells

2009 ◽  
Vol 4 (2) ◽  
pp. 61-68
Author(s):  
Dmitriy Strokotov ◽  
Yuriy Pichugin ◽  
Maxim Yurkin ◽  
Mariya Gridina ◽  
Oleg Serov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Light Scattering ◽  
Mononuclear Cells ◽  
Human Lymphocytes ◽  
Scattering Problem ◽  
Refractive Indices ◽  
Single Particles ◽  
Differentiation Status

In this manuscript we propose two methods to solve inverse light scattering problem for single particles, which can be described as a coated sphere. The efficiency of the methods is illustrated by characterization of lymphocytes and stem cells using light scattering patterns obtained with scanning flow cytometer. Both methods, spectral and global optimization, were used to obtain diameters and refractive indices of the cytoplasm and the nucleus of mice embryo stem cells and human lymphocytes. These results agree with data obtained from other studies. Determination of these parameters is important for diagnostics of pathological states of lymphocytes and differentiation status of embryo stem cells. Moreover, methods described in this manuscript are applicable to all mononuclear cells. We also considered limitations of these methods and their possible improvements.

Collaborative Study of a Method for the Determination of Ochratoxin A in Green Coffee

1975 ◽  
Vol 58 (2) ◽  
pp. 258-262 ◽  
Author(s):  
Colette P Levi
Keyword(s):  
Thin Layer ◽  
Ochratoxin A ◽  
Collaborative Study ◽  
Green Coffee ◽  
Average Recovery ◽  
Semiquantitative Determination ◽  
Chromatographic Methods ◽  
Green Coffee Beans ◽  
Coffee Beans

Abstract A method for the semiquantitative determination of ochratoxin A in green coffee has been studied collaboratively by 11 laboratories. The average recovery for the 7 samples spiked at 3 levels of ochratoxin A was 69.1%, ranging from 60.5 to 85.6%. This is comparable to other visual thin layer chromatographic methods of mycotoxin detection. The method has been adopted as official first action for the determination of ochratoxin A in green coffee beans.

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