ON THE MECHANISM OF THE RING ZONE EFFECT OBTAINED WITH THE MIXED HAEMADSORPTION TECHNIQUE

The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) protein p74 is associated with the occlusion-derived virus (ODV) envelope. p74 is essential for oral infectivity of ODV and has been proposed to play a role in midgut attachment and/or fusion. In this study, p74 protein was expressed in-frame with green fluorescent protein (GFP) to create a p74–GFP chimera. The C-terminal GFP portion of the chimera facilitated visualization of the trafficking of p74 in baculovirus-infected Spodoptera frugiperda (Sf-9) cells. p74–GFP chimeric proteins localized in the intranuclear ring zone of the nucleus and were found to co-precipitate with the microvesicle fraction of cell lysates. A series of truncations of p74 was expressed as p74–GFP chimeras in recombinant baculoviruses. When C-terminal region S580–F645 was deleted from p74, p74–GFP chimera localization became non-specific and chimeras became soluble. p74 region S580–F645 directed GFP to the intranuclear ring zone in a similar pattern to full-length p74. The hydrophobic C terminus of p74 plays a role in protein localization and possibly in transmembrane anchoring and insertion.

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City fringe area landscape ecological planning under the green ring - Green wedge mode - To set the ecological ring zone of Chengdu as an example

Proceedings of the 2015 International Conference on Social Science and Technology Education ◽

10.2991/icsste-15.2015.184 ◽

2015 ◽

Author(s):

Han Junjie ◽

Hou Chaoping ◽

Liu Jiawen

Keyword(s):

Ecological Planning ◽

Green Ring ◽

Ring Zone ◽

Landscape Ecological Planning ◽

Landscape Ecological ◽

Fringe Area

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Proposal for an electrostatic energy filter and a monochromator

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100107393 ◽

1978 ◽

Vol 36 (1) ◽

pp. 50-51

Author(s):

Erich Plies

Keyword(s):

Angular Momentum ◽

Coordinate System ◽

Axial Symmetry ◽

Relativistic Effects ◽

Optic Axis ◽

Electrostatic Energy ◽

Cylinder Axis ◽

Ring Zone ◽

Cylindrical Mirror ◽

Fringing Fields

Both instruments suggested here are composed of several cylindrical mirror analyzers (CMA) with the centres of object and image on the cylinder axis, see fig. 1. Usually the whole 360° ring zone of the CMA is used but in our case the electrons fly only within a segment of the ring zone. In the curvilinear xyz coordinate system of this bundle, there exist five fundamental rays xα, yβ,xγ,yδ and xϰ (fig. 1). As is well-known from [1], 2nd-order focusing occurs in the first principal section if ⊝=42.31° and R2/R1 = 3.705 (relativistic effects and fringing fields neglected). 50kV electrons need ⊝=43.07° and R2/R13.660. Due to the axial symmetry, the beam is also focused in 2nd order in the 2nd principal section. If one shifts the curved optic axis in the Z-direction, one obtains the ray γxγ.The trajectory is found using Helmholtz's theorem and the conservation of the Z-component of the angular momentum.

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