Physicochemical properties of water-soluble myofibrillar proteins prepared from chicken breast muscle

2004 ◽  
Vol 75 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Yukiko ITO ◽  
Shinji TOKI ◽  
Takashi OMORI ◽  
Hiroshi IDE ◽  
Ryuichi TATSUMI ◽  
...  
Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 511
Author(s):  
Evelin Kivima ◽  
Kristel Tanilas ◽  
Kaie Martverk ◽  
Sirli Rosenvald ◽  
Loreida Timberg ◽  
...  

Thirty honey samples from different regions of Estonia were investigated to determine the chemical compositions, physicochemical properties, bioactive compounds, and sensory characteristics of typical honeys from a northern climate. The physicochemical parameters, such as electrical conductivity, moisture content, free acidity, hydroxymethylfurfural, diastase, and invertase activity were measured. The color was measured and expressed by L*-, a*-, and b*-coordinates. Sensory parameters were determined by using “fruity”, “floral”, “berry-like”, “herbal”, “woody”, “spicy”, “sweet”, and “animal-like” as the main odor and flavor attributes. The total polyphenol and flavonoid contents were in the range of 26.2–88.7 mg gallic acid equivalents (GAE) per 100 g and 1.9–6.4 mg quercetin equivalents (QE) per 100 g, respectively. The identified polyphenols showed the highest intensities of caffeic acid, coumaric acid, and abscisic acid and its derivatives. The protocatechuic acid intensity was highest in honeys containing traces of honeydew elements and of cinnamic acid and myricetin in heather honey. The water-soluble antioxidant values were 37.8–311.2 mg ascorbic acid equivalents (AAE) per 100 g and the lipid soluble antioxidant values were 14.4–60.7 mg Trolox equivalents (TE) per 100 g. The major amino acid in the analyzed honeys was proline, with variable values depending on the honey’s botanical source. Correlations were calculated based on the results obtained. It was revealed that the typical Estonian honey has floral, berry-like, sweet, and rather mild sensory characteristics. Most of the honeys lacked stronger spicy, woody, and animal-like attributes. The typical color of Estonian honey is quite light.


1963 ◽  
Vol 41 (1) ◽  
pp. 369-387 ◽  
Author(s):  
J. M. Neelin

By varying conditions of starch gel electrophoresis, factors contributing to the resolution of myogen proteins from chicken breast muscle have been studied. Variables examined included composition of the myogen extractant, protein concentration, ionic strength of electrophoretic media, pH of gel media, plane and direction of electrophoresis, and the nature of cations and anions in gel media and bridge solutions. The significance of anions was more closely studied with constant buffer systems, and gradient systems in which bridge electrolyte differed from, and gradually altered, the gel medium. Optimal separation was obtained in gradient systems with 0.10 M sodium chloride bridge solutions, and gel media of sodium cacodylate, pH 6.9, μ 0.010, which resolved 12 cationic zones, and sodium veronal, pH 7.4, μ 0.010, which resolved 10 anionic zones. These buffers in two-dimensional sequence revealed a total of about 24 components in this myogen.


1972 ◽  
Vol 50 (10) ◽  
pp. 1132-1142 ◽  
Author(s):  
Eric James ◽  
R. O. Hurst ◽  
T. G. Flynn

Phosphoglyceromutase (2,3-diphospho-D-glycerate: 2-phospho-D-glycerate phosphotransferase, EC 2.7.5.3) has been purified from both frozen and fresh chicken breast muscle. During purification it was found that substrate, 3-phospho-D-glycerate stabilized the enzyme against heat inactivation to almost the same extent as did the cofactor 2,3-diphospho-D-glycerate.Phosphoglyceromutase prepared from frozen chicken breast muscle separated into three peaks of activity (I, II, and III) following chromatography on DEAE-Sephadex in 0.05 μ phosphate buffer, pH 8.0, using a 0.0–0.4 M NaCl gradient. Each peak of activity was shown by polyacrylamide disc gel electrophoresis at pH 9.3 to contain two enzymically active components (isoenzymes Ia Ib, IIa IIb, and IIIa IIIb). Isoenzymes in the same peak had the same specific activity. Phosphoglyceromutase prepared from fresh chicken breast muscle yielded only one peak of activity following chromatography on DEAE-Sephadex. This peak contained two enzymically active components corresponding to isoenzymes Ia and Ib. Additional peaks of activity were not produced when phosphoglyceromutase from fresh muscle was subjected to freezing and thawing.Isoenzyme Ia and mixtures of Ia and Ib, IIa and IIb, and IIIa and IIIb were homogeneous in the ultra-centrifuge sedimenting as single peaks. The sedimentation coefficient obtained for isoenzyme Ia and for Ia and Ib combined was 4.15 S, the diffusion constant 6.62 × 10−7 cm2/s, and the molecular weight calculated from both gel filtration and sedimentation data was of the order of 59 000. These results were confirmed by charge isomer studies which also showed that the isoenzymes of phosphoglyceromutase from frozen chicken breast muscle were proteins of the same size but different net charges.


INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 39-47
Author(s):  
V. K Sharma ◽  
◽  
B. Mazumder ◽  
P. P. Sharma

The consumption of edible products strongly recommends the regular hygiene of oral cavity. Various dental products of allopathic and herbal origin are used as dentifrices. The dentifrices are considered safe and effective in terms of cleansing effect of oral cavity and antimicrobial effect against microbes causing bad smell and diseases such as gingivitis, pyorrhea etc. These characteristics of preparations are basically related to physicochemical properties of ingredients present in their composition and some how on directions of their use. In the present study, the marketed allopathic dentifrices coded as Brand I and II and herbal tooth powders coded as Brand III and IV were selected to analyze the impact of physicochemical properties of incorporated ingredients on their cleansing efficiency. The physicochemical characteristics studied were pH, bulk volume, tapped volume, tapped density, bulk density, true density, porosity, flowability, compressibility, compactability, cohesiveness, dispersability, Carr’s index, Hausner’s ratio, water soluble content, alcohol soluble content, foaming index, particle rearrangement behaviour and particle rearrangement constant. The antimicrobial effect of these powders was studied against Staphylococcus sorbinus, Staphylococcus salivarius and Lactobacillus acidophilus. It was observed that some of the physicochemical properties of all powders were different from each other. Marked antimicrobial effect of tooth powders was observed against pathogens. In all preparations, remarkable foaming index was analyzed that was generally considered responsible for cleansing effect.


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