Hair Growth and Hair Follicle Cell Proliferation in Histocultured Mouse Skin

Extractions from Phaeodactylum tricornutum have been widely studied and evaluated to various biological effects. The aim of this study was to investigate the promotional effect of P. tricornutum extract (PTE) on the ERK1/2 signaling pathway involved in hair follicle cell proliferation. In order to illuminate the enhancement of PTE on hair growth by promoting proliferation of hair follicle cells, the activities of human hair follicle outer root sheath cell (HFORSC), human hair follicle germinal matrix cells (HFGMC), and hair epithelial melanocytes (HEM) were observed under PET treatment. Levels of keratins, PKCζ, ERK1/2, and p38 MAPK in hair follicle cells were determined by Western blotting to illustrate the mechanisms of PTE effects on hair growth. Analyzed by GC-MS, the main polyunsaturated fatty acids which were 9.43% of total fatty acids in PTE were linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid. Melanin content and tyrosinase activity in HEM were measured. The results showed that PTE exhibited remarkable enhancement on cell proliferation. Melanin production was inhibited by PTE treatment, while keratin-14, keratin-15, and keratin-17 levels on hair follicle cells were elevated at different concentrations. The promotions of ERK1/2 and p38 MAPK levels indicated that the ERK1/2 signaling pathway is involved in the proliferation of hair follicle cells. These results are the evidence that PTE potentially deserves further study as a new natural candidate for hair care applications.

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HNG, A Humanin Analogue, Promotes Hair Growth by Inhibiting Anagen-to-Catagen Transition

International Journal of Molecular Sciences ◽

10.3390/ijms21124553 ◽

2020 ◽

Vol 21 (12) ◽

pp. 4553

Author(s):

Sung Min Kim ◽

Jung-Il Kang ◽

Hoon-Seok Yoon ◽

Youn Kyung Choi ◽

Ji Soo Go ◽

...

Keyword(s):

Hair Follicle ◽

Hair Growth ◽

Cell Cycle Progression ◽

Dermal Papilla ◽

Follicle Cell ◽

Hair Shaft ◽

Promoting Effect ◽

Dermal Papilla Cells ◽

Anagen Phase

The hair follicle goes through repetitive cycles including anagen, catagen, and telogen. The interaction of dermal papilla cells (DPCs) and keratinocytes regulates the hair cycle and hair growth. Humanin was discovered in the surviving brain cells of patients with Alzheimer’s disease. HNG, a humanin analogue, activates cell growth, proliferation, and cell cycle progression, and it protects cells from apoptosis. This study was performed to investigate the promoting effect and action mechanisms of HNG on hair growth. HNG significantly increased DPC proliferation. HNG significantly increased hair shaft elongation in vibrissa hair follicle organ culture. In vivo experiment showed that HNG prolonged anagen duration and inhibited hair follicle cell apoptosis, indicating that HNG inhibited the transition from the anagen to catagen phase mice. Furthermore, HNG activated extracellular signal-regulated kinase (Erk)1/2, Akt, and signal transducer and activator of transcription (Stat3) within minutes and up-regulated vascular endothelial growth factor (VEGF) levels on DPCs. This means that HNG could induce the anagen phase longer by up-regulating VEGF, which is a Stat3 target gene and one of the anagen maintenance factors. HNG stimulated the anagen phase longer with VEGF up-regulation, and it prevented apoptosis by activating Erk1/2, Akt, and Stat3 signaling.

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Growth factors specifically alter hair follicle cell proliferation and collagenolytic activity alone or in combination

Differentiation ◽

10.1111/j.1432-0436.1990.tb00470.x ◽

1990 ◽

Vol 45 (3) ◽

pp. 168-178 ◽

Cited By ~ 40

Author(s):

Wendy C. Weinberg ◽

Peter D. Brown ◽

William G. Stetler-Stevenson ◽

Stuart H. Yuspa

Keyword(s):

Cell Proliferation ◽

Growth Factors ◽

Hair Follicle ◽

Follicle Cell ◽

Collagenolytic Activity

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Modulation of Hair Follicle Cell Proliferation and Collagenolytic Activity by Specific Growth Factorsa

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1991.tb24394.x ◽

2006 ◽

Vol 642 (1) ◽

pp. 281-290 ◽

Cited By ~ 10

Author(s):

WENDY C. WEINBERG ◽

PETER D. BROWN ◽

WILLIAM G. STETLER-STEVENSON ◽

STUART H. YUSPA

Keyword(s):

Cell Proliferation ◽

Hair Follicle ◽

Specific Growth ◽

Follicle Cell ◽

Collagenolytic Activity

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Photoactivation of ROS Production In Situ Transiently Activates Cell Proliferation in Mouse Skin and in the Hair Follicle Stem Cell Niche Promoting Hair Growth and Wound Healing

Journal of Investigative Dermatology ◽

10.1038/jid.2015.248 ◽

2015 ◽

Vol 135 (11) ◽

pp. 2611-2622 ◽

Cited By ~ 31

Author(s):

Elisa Carrasco ◽

María I. Calvo ◽

Alfonso Blázquez-Castro ◽

Daniela Vecchio ◽

Alicia Zamarrón ◽

...

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Stem Cell ◽

Hair Growth ◽

Stem Cell Niche ◽

Mouse Skin ◽

Ros Production ◽

Hair Follicle Stem Cell ◽

Cell Niche

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Hair Growth-Promoting Activity of Citron Seed Oil: Induction of Premature Telogen-To-Anagen Transition of Hair Follicle in C57BL/6 Mouse Models

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.20:28-36 ◽

2021 ◽

Vol 20 (1) ◽

pp. 28-36

Author(s):

Van-Long Truong ◽

Woo-Sik Jeong

Keyword(s):

Growth Factor ◽

Hair Follicle ◽

Hair Growth ◽

Seed Oil ◽

Growth Promotion ◽

Mouse Skin ◽

Raw Material ◽

Skin Thickness ◽

Citrus Junos ◽

Growth Promoting

Citron (Citrus junos Sieb. Ex Tanaka), a citrus fruit native to Korea, Japan, and China, has been commonly used as a raw material for beverages and herbal medicines. Citron seeds, the by-product of processing industry, contain many bioactive compounds that exhibit several biological properties, such as antioxidative, anti-inflammatory, and antiobesity. However, potential of citron seed oil for hair growth promotion remains unexplored. In the present study, we investigated the hair growth-promoting effect of citron seed oil and its underlying mechanism(s) in a C57BL/6 mouse model. The results show that citron seed oil effectively promotes hair regrowth by early induction of telogen-to-anagen transition and by increasing hair follicle density and hair bulb diameter as well as skin thickness. Citron seed oil treatment increased β-catenin, lymphoid enhancer binding factor 1, sonic hedgehog, cyclin D1 and cyclin E expressions, and alkaline phosphatase activity, compared to the vehicle-treated control mice. Topical administration of citron seed oil upregulated expression of antiapoptotic Bcl-2 protein but did not alter protein level for proapoptotic Bax in mouse skin tissues. Furthermore, insulin-like growth factor 1 and vascular endothelial growth factor were also induced by citron seed oil treatment. These findings suggest the potential of citron seed oil in the prevention and/or treatment of hair loss by inducing hair cycling and regeneration.

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