Evaluation of an enzyme-linked immunosorbent assay for the detection of Mycoplasma hyopneumoniae antibody in porcine serum

1992 ◽  
Vol 69 (10) ◽  
pp. 255-258 ◽  
Author(s):  
RF SHELDRAKE ◽  
LF ROMALIS
Keyword(s):  
Immunosorbent Assay ◽  
Mycoplasma Hyopneumoniae ◽  
Enzyme Linked Immunosorbent Assay ◽  
Porcine Serum

scholarly journals Automation and ISO 17025 Test Validation of a Toxoplasma Gondii Antibodies Enzyme-Linked Immunosorbent Assay

2003 ◽  
Vol 8 (5) ◽  
pp. 42-50
Author(s):  
John T. Y. Wu ◽  
Lester S. Y. Wong ◽  
Eva Y. W. Chow ◽  
Evelyn E. Bowlby
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
High Throughput Screening ◽  
Fold Increase ◽  
Threshold Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Iso 17025 ◽  
Test Configuration ◽  
Porcine Serum ◽  
Panel Testing

The authors automated an enzyme-linked immunosorbent assay to detect porcine serum antibodies to Toxoplasma gondii. Two thousand swine sera can be assayed in two eight-hour shifts using a robotic workstation. The automated-ELISA programming is not complex and the test configuration is flexible. This high-throughput screening (HTS) a-ELISA can achieve a 10-fold increase (100→ 1000 tests) in test capacity over the manual method. The assay has been validated according to the requirements of the ISO/IEC 17025 standard. These include repeatability, reproducibility, and optimal threshold value studies. Other requirements are proficiency panel testing, analyst training, standard operation procedure, and equipment certifications.

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