A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) Strain Tolerant to High Concentration of Calcium Chloride

2021 ◽  
Author(s):  
Xudong Liu ◽  
Jinli Zhao ◽  
Jia Feng ◽  
Junping Lv ◽  
Qi Liu ◽  
...  
Keyword(s):  
Calcium Chloride ◽  
High Concentration ◽  
Parachlorella Kessleri

scholarly journals TESTING OF A DEFLUORIDATING FILTER USING MgO-CaO-CaCI2 FOR USE IN RURAL RAJASTHAN

2013 ◽  
Vol 21 (21) ◽  
pp. 79-95
Author(s):  
K. Margandan ◽  
R. Agrawal ◽  
K. Singh ◽  
R. Acharya ◽  
S. Sharma ◽  
...  
Keyword(s):  
Magnesium Oxide ◽  
Calcium Chloride ◽  
High Concentration ◽  
Bicarbonate Concentration ◽  
Ph Adjustment ◽  
High Alkalinity

A Magnesium Oxide-based defluoridation technique using MgO-CaO-Calcium Chloride and pH adjustment by Sodium Bisulphate has been suitably modified for use with fluoride contaminated local groundwater having high alkalinity. Taking into consideration the high bicarbonate concentration in raw groundwater the proportion of the lime has been changed. It was found that sodium bisulphate was not suitable for pH adjustment due to the high concentration of sulphate in the pH adjusted water. Therefore dilute HCl was used instead. Six trials of the adapted defluoridation technique have been carried out in the laboratory successfully at a 50-litre scale.

scholarly journals Screening supplements to enhance the biological control activity of HR42 against Colletotrichum acutatum

2013 ◽  
Vol 66 ◽  
pp. 383-383
Author(s):  
M.J. Vergara ◽  
N.J. Larsen ◽  
I.P.S. Pushparajah ◽  
K.R. Everett
Keyword(s):  
Biological Control ◽  
Calcium Chloride ◽  
Biological Control Agent ◽  
Fungal Growth ◽  
Control Agent ◽  
Ammonium Molybdate ◽  
Colletotrichum Acutatum ◽  
High Concentration ◽  
Glycol Chitosan ◽  
Control Activity

Ten compounds were evaluated for their effects on the efficacy of the bacterial biological control agent (BCA) HR42 against the avocado postharvest rot fungus Colletotrichum acutatum The effects of the 10 compounds (chitin Dglucose glycol chitosan Lserine Laspartic acid calcium chloride calcium carbonate potassium chloride ammonium molybdate and sodium carbonate) at four different concentrations (1 10 100 and 1000 mM) were tested separately on bacterial and fungal growth Lserine Laspartic acid and calcium chloride all stimulated growth of HR42 and suppressed mycelial growth of C acutatum The BCA and these compounds were then tested in combination on detached avocado fruit for their ability to inhibit lesion development following woundinoculation A high concentration of HR42 (107 cfu/ml) significantly inhibited the development of lesions caused by 105 and 107 conidia/ml of C acutatum There was no improvement of control by the addition of any of the three compounds These results show that HR42 has biocontrol activity against C acutatum in a detached fruit test but there was no enhancement of control by the addition of supplements

Cytomembrane Preservation in Tissue Dehydrated Only With Glutaraldehyde and Epon 812 Resin

1973 ◽  
Vol 31 ◽  
pp. 320-321
Author(s):  
Daniel C. Pease
Keyword(s):  
Diffraction Pattern ◽  
X Ray Diffraction ◽  
High Concentration ◽  
Unpublished Study ◽  
X Ray ◽  
Sectioned Material

A previous study demonstrated that tissue could be successfully infiltrated with 50% glutaraldehyde, and then subsequently polymerized with urea to create an embedment which retained cytomembrane lipids in sectioned material. As a result, the 180-190 Å periodicity characteristic of fresh, mammalian myelin was preserved in sections, as was a brilliant birefringence, and the capacity to bind OsO4 vapor in the hydrophobic bilayers. An associated (unpublished) study, carried out in co-operation with Drs. C.K. Akers and D.F. Parsons, demonstrated that the high concentration of glutaraldehyde (and urea) did not significantly alter the X-ray diffraction pattern of aldehyde-fixed, myelin. Thus, by itself, 50% glutaraldehyde has little effect upon cytomembrane systems and can be used with confidence for the first stages of dehydration.

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

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