scholarly journals Inactivation of vegetative cells, germinated spores, and dormant spores of Bacillus atrophaeus by pulsed electric field with fixed energy input

Author(s):  
Wen Jie Wu ◽  
Jinhui Chang
2020 ◽  
Vol 10 (4) ◽  
pp. 12-21
Author(s):  
I.A. Shorstky ◽  
◽  
O. Parniakov ◽  
S. Smetana ◽  
◽  
...  

The perspectives of pulsed electric field (PEF) application for larvae biomass drying are considered. Drying process optimization was carried out using two-way analysis of variance in the range of applied specific energy input of from 0 up to 20 kJ/kg and drying temperature of from 50 up to 90°С. It was found out that application of pulsed electric field treatment allowed marked decreasing of larvae biomass drying time from 183 to 124 minutes for the samples treated with electric filed intensity of E = 2 kV/cm and specific energy of 20 kJ/kg. Based on the obtained experimental data the optimal drying and PEF treatment parameters for larvae biomass were determined for the ranges of drying temperature – 82–85℃ and specific energy input – 4.1–6.6 kJ/kg.


2000 ◽  
Vol 66 (1) ◽  
pp. 428-430 ◽  
Author(s):  
Irene E. Pol ◽  
Hennie C. Mastwijk ◽  
Paul V. Bartels ◽  
Eddy J. Smid

ABSTRACT Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 μg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-μs duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatments, indicating synergy.


2001 ◽  
Vol 67 (4) ◽  
pp. 1693-1699 ◽  
Author(s):  
Irene E. Pol ◽  
Willy G. C. van Arendonk ◽  
Hennie C. Mastwijk ◽  
Judit Krommer ◽  
Eddy J. Smid ◽  
...  

ABSTRACT Treatment of Bacillus cereus spores with nisin and/or pulsed-electric-field (PEF) treatment did not lead to direct inactivation of the spores or increased heat sensitivity as a result of sublethal damage. In contrast, germinating spores were found to be sensitive to PEF treatment. Nisin treatment was more efficient than PEF treatment for inactivating germinating spores. PEF resistance was lost after 50 min of germination, and not all germinated spores could be inactivated. Nisin, however, was able to inactivate the germinating spores to the same extent as heat treatment. Resistance to nisin was lost immediately when the germination process started. A decrease in the membrane fluidity of vegetative cells caused by incubation in the presence of carvacrol resulted in a dramatic increase in the sensitivity to nisin. On the other hand, inactivation by PEF treatment or by a combination of nisin and PEF treatments did not change after adaptation to carvacrol. Spores grown in the presence of carvacrol were not susceptible to nisin and/or PEF treatment in any way.


2013 ◽  
Vol 133 (2) ◽  
pp. 32-37 ◽  
Author(s):  
Akira Nakagawa ◽  
Hitoshi Hatayama ◽  
Koichi Takaki ◽  
Shoji Koide ◽  
Yukio Kawamura

2015 ◽  
pp. 758-760
Author(s):  
Romain Delecourt ◽  
Loïc Marsal

Maguin (France) is still active in the application of pulsed electric field (PEF) technology. After having carried out successful tests on a 10 t/h pilot screw-type machine on sugar beet cossettes, a new application system based on a roller technology has been developed. This technology allows a wide range of application due to its flexibility with flowrates and materials. A variety of process schemes are proposed to ensure the best performance of the PEF technology.


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