Effect of temperature on thermal denaturation of skimmed coconut milk to produce a new product, coconut water

Author(s):  
Phiraiwan Jermwongruttanachai ◽  
Siwalak Pathaveerat ◽  
Sirinad Noypitak
2019 ◽  
Vol 26 (7) ◽  
pp. 532-541 ◽  
Author(s):  
Cadena-Cadena Francisco ◽  
Cárdenas-López José Luis ◽  
Ezquerra-Brauer Josafat Marina ◽  
Cinco-Moroyoqui Francisco Javier ◽  
López-Zavala Alonso Alexis ◽  
...  

Background: Cathepsin D is a lysosomal enzyme that is found in all organisms acting in protein turnover, in humans it is present in some types of carcinomas, and it has a high activity in Parkinson's disease and a low activity in Alzheimer disease. In marine organisms, most of the research has been limited to corroborate the presence of this enzyme. It is known that cathepsin D of some marine organisms has a low thermostability and that it has the ability to have activity at very acidic pH. Cathepsin D of the Jumbo squid (Dosidicus gigas) hepatopancreas was purified and partially characterized. The secondary structure of these enzymes is highly conserved so the role of temperature and pH in the secondary structure and in protein denaturation is of great importance in the study of enzymes. The secondary structure of cathepsin D from jumbo squid hepatopancreas was determined by means of circular dichroism spectroscopy. Objective: In this article, our purpose was to determine the secondary structure of the enzyme and how it is affected by subjecting it to different temperature and pH conditions. Methods: Circular dichroism technique was used to measure the modifications of the secondary structure of cathepsin D when subjected to different treatments. The methodology consisted in dissecting the hepatopancreas of squid and freeze drying it. Then a crude extract was prepared by mixing 1: 1 hepatopancreas with assay buffer, the purification was in two steps; the first step consisted of using an ultrafiltration membrane with a molecular cut of 50 kDa, and the second step, a pepstatin agarose resin was used to purification the enzyme. Once the enzyme was purified, the purity was corroborated with SDS PAGE electrophoresis, isoelectric point and zymogram. Circular dichroism is carried out by placing the sample with a concentration of 0.125 mg / mL in a 3 mL quartz cell. The results were obtained in mdeg (millidegrees) and transformed to mean ellipticity per residue, using 111 g/mol molecular weight/residue as average. Secondary-structure estimation from the far-UV CD spectra was calculated using K2D Dichroweb software. Results: It was found that α helix decreases at temperatures above 50 °C and above pH 4. Heating the enzyme above 70°C maintains a low percentage of α helix and increases β sheet. Far-UV CD measurements of cathepsin D showed irreversible thermal denaturation. The process was strongly dependent on the heating rate, accompanied by a process of oligomerization of the protein that appears when the sample is heated, and maintained a certain time at this temperature. An amount typically between 3 and 4% α helix of their secondary structure remains unchanged. It is consistent with an unfolding process kinetically controlled due to the presence of an irreversible reaction. The secondary structure depends on pH, and a pH above 4 causes α helix structures to be modified. Conclusion: In conclusion, cathepsin D from jumbo squid hepatopancreas showed retaining up to 4% α helix at 80°C. The thermal denaturation of cathepsin D at pH 3.5 is under kinetic control and follows an irreversible model.


2017 ◽  
Vol 5 (2) ◽  
pp. 149 ◽  
Author(s):  
Amadi Benjamin ◽  
Ogunka-Nnoka Charity ◽  
Amadi Peter ◽  
Ogaji Miebaka

Using standard methods, this study investigated the ameliorative effects of coconut products during alloxan-induced diabetic conditions. Experimental animals were divided into five groups, group 1 served as normal control rats fed only rat chow and saline, group 2 were dia-betic control rats intraperitoneally treated with 150mg/kg body weight alloxan monohydrate, group 3 were diabetic rats orally treated with 4ml/day of coconut milk, group 4 were diabetic rats orally treated with 4ml/day of coconut water, and group 5 were diabetic rats orally treat-ed with 4ml/day of a mixture of coconut milk and coconut water. The coconut products had high moisture, fats, potassium, magnesium, and sodium contents. Coconut milk exhibited the most effective glucose lowering effect, and on the 21st day. The total cholesterol was com-pletely normalized on treatment with coconut milk after alloxan induced diabetes, while the administration of the mixture of coconut milk and water had a comparable effect to administering only coconut milk on HDL, LDL, and TG. The alloxan-induced derangements on SOD, catalase and GPx were completely normalized after the coconut milk administration, while the mixture of coconut milk and water restored only SOD and GPx, and coconut water, ineffective on most of the antioxidant enzymes. Coconut water was ineffective on the RBC and HB of diabetic rats, while coconut milk and the mixture of coconut milk and water showed the most hemato-ameliorative effect. This study has shown the effectiveness of coconut products in the management of diabetes, with coconut milk the most effective.


Pro Food ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 665
Author(s):  
Lucia Cecilia Mandey ◽  
Dantje Tarore ◽  
Jenny E.A. Kandou ◽  
Natasia M. Dumais

ABSTRACT Production research technology of nata de coco organic aims to find an alternative nitrogen source model to replace Urea Fertilizer (ZA) to produce quality nata de coco products and to implement this model in the community (UKM NATA de COCO). The benefit of this research is to produce quality nata de coco products and contain a source of fiber (dietary fiber). The quantitative Research Methods (laboratory experiments), with Phase I research carried out trough laboratory test with Treatment A = 10 liters of coconut water + 50 ml of bean sprouts extract; Treatment B = 10 liters of coconut water + 50 ml mung bean extract; Treatment C = basic ingredients of coconut water 10 liters + coconut milk 50 ml and Treatment D = basic ingredients of coconut water 10 liters + ZA Food Grade 20 grams + the palm tree sap and treatment E = basic ingredients of coconut water + ZA Non FOod Grade (control). The quality parameters of nata de coco studied were water content, rendemen, crude fiber content, thickness, color and weight of the pellicle. Next Phase II Research: Implementation of Alternative Nitrogen Substituted Urea Fertilizer (ZA) source models in Natural Cellulose Small Business (nata de Coco) in Airmadidi District, North Minahasa Regency. The results of the research: (1). Nitrogen sources are obtained from natural / organic ingredients such as: bean sprouts extract, green bean extract, coconut milk, as an alternative to Urea (ZA) fertilizer which can be used by bacteria Acetobacter xylinum to form natural cellulose pellicles, and a source of Acetobacter xylinum from the sap of the Enau tree. (2). A quality nata de coco product is obtained by providing a model for the use of “Food Grade” N sources in small businesses that produce nata de coco in Airmadidi District, North Minahasa Regency.   Keywords: dietary fiber, nata de coco, organic   ABSTRAK         Penelitian Teknologi Produksi Nata De Coco Berbahan Baku Organik   bertujuan untuk mendapatkan model sumber nitrogen alternatif pengganti Pupuk Urea (ZA) guna menghasilkan produk nata de coco yang berkualitas serta dapat mengimplementasikan model ini kepada masyarakat (UKM Nata de Coco).  Manfaat penelitian ini guna menghasilkan produk nata de coco yang berkualitas dan mengandung sumber serat (dietary fiber).  Metode Penelitian secara kuantitatif (eksperimen laboratorium), dengan Penelitian Tahap I dilakukan melalui uji laboratorium dengan  Perlakuan A =  bahan dasar air kelapa 10 liter + ekstrak tauge 50 ml; Perlakuan B = bahan dasar air kelapa 10 liter + ekstrak kacang hijau 50 ml; Perlakuan C = bahan dasar air kelapa 10 liter + santan kelapa 50 ml dan Perlakuan D = bahan dasar air kelapa 10 liter + ZA Food Grade 20 Gram + Nira Pohon Enau dan Perlakuan E = bahan dasar air kelapa + ZA Non Food Grade (Kontrol).Parameter kualitas nata de coco yang diteliti yaitu: kadar air, rendeman, kadar serat kasar, ketebalan,   warna dan berat pelikel.  Selanjutnya Penelitian Tahap II: Implementasi model sumber nitrogen alternatif pengganti Pupuk Urea (ZA) pada Usaha Kecil nata de coco di Kecamatan Airmadidi Kabupaten Minahasa Utara. Hasil penelitian  yang dihasilkan: (1).  Diperoleh   Sumber Nitrogen dari bahan alami/organik seperti : ekstrak tauge, ekstrak kacang  hijau, santan kelapa,  sebagai alternatif pengganti   Pupuk Urea (ZA) yang dapat digunakan  oleh bakteri Acetobacter xylinum dalam  menmbentuk pelikel selulosa alami, dan sumber Acetobacter xylinum dari nira pohon Enau. (2). Diperoleh   produk  nata de coco   yang  berkualitas dengan memberikan  model penggunaan sumber N “Food Grade” pada Usaha Kecil yang   memproduksi nata de  coco  di Kecamatan Airmadidi Kabupaten   Minahasa Utara.   Kata kunci: dietary fiber, nata de coco, organik


2019 ◽  
Vol 16 ◽  
pp. 2238-2244 ◽  
Author(s):  
Siti Fatimah Rahamat ◽  
Wan Nor Hayati Wan Abd Manan ◽  
Anil Azura Jalaludin ◽  
Zurainie Abllah

2019 ◽  
Vol 284 ◽  
pp. 259-263 ◽  
Author(s):  
Luciane B. Paixão ◽  
Geovani C. Brandão ◽  
Rennan Geovanny O. Araujo ◽  
Maria Graças A. Korn

2010 ◽  
Vol 2 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Bambang Setiaji ◽  
Ani Setyopratiwi ◽  
Nahar Cahyandaru

A research to know influence of mixing concentration of coconut water and sucrose concentration to coconut milk skim as nata de coco substrate has been conducted. The variation was taken from mixing coconut water (0%, 25%, 35% and 50% and 100% as control) and the sucrose concentration (0.5%, 1%, 1.5% and 2%). Coconut milk skim boiled before used as substrat, yielded a coconut protein (blondo). The result of research showed that coconut milk skim can be used as nata de coco substrate with mixing coconut water and sucrose addition, mixing 50 % concentration coconut water representing optimum concentration. The content of crude fibre nata was yielded by higher concentration of sucrose, while mixing concentration coconut water do not influence crude fibre content.   Keyword: Coconut milk skim, substrate, nata de coco


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