Iron oxide nanoparticles decrease nuclear fractal dimension of buccal epithelial cells in a time-dependent manner

Ultrasmall iron oxide nanoparticles (<10 nm) were loaded with cis-diamminetetrachloroplatinum (IV), a cisplatin (II) prodrug, and used as an efficient nanodelivery system in cell models. To gain further insight into their behavior in ovarian cancer cells, the level of cellular incorporation as well as the platination of mitochondrial and nuclear DNA were measured using inductively coupled plasma mass spectrometry (ICP-MS) strategies. Quantitative Pt results revealed that after 24 h exposure to 20 µM Pt in the form of the Pt(IV)-loaded nanoparticles, approximately 10% of the incorporated Pt was associated with nuclear DNA. This concentration increased up to 60% when cells were left to stand in drug-free media for 3 h. These results indicated that the intracellular reducing conditions permitted the slow release of cisplatin (II) from the cisplatin (IV)-loaded nanoparticles. Similar results were obtained for the platination of mitochondrial DNA, which reached levels up to 17,400 ± 75 ng Pt/ mg DNA when cells were left in drug-free media for 3 h, proving that this organelle was also a target for the action of the released cisplatin (II). The time-dependent formation of Pt-DNA adducts could be correlated with the time-dependent decrease in cell viability. Such a decrease in cell viability was correlated with the induction of apoptosis as the main route of cell death. The formation of autophagosomes, although observed upon exposure in treated cells, does not seem to have played an important role as a means for cells to overcome nanoparticles’ toxicity. Thus, the designed nanosystem demonstrated high cellular penetration and the “in situ” production of the intracellularly active cisplatin (II), which is able to induce cell death, in a sustained manner.

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Synergistic Effect of Vitamin C with Superparamagnetic Iron Oxide Nanoparticles for Inhibiting Proliferation of Gastric Cancer Cells

Biointerface Research in Applied Chemistry ◽

10.33263/briac123.32153224 ◽

2021 ◽

Vol 12 (3) ◽

pp. 3215-3224

Keyword(s):

Gene Expression ◽

Iron Oxide ◽

Vitamin C ◽

Iron Oxide Nanoparticles ◽

Superparamagnetic Iron Oxide ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Dependent Manner ◽

Low Doses ◽

Gastric Cancer Cells

The present study aimed to explore the synergistic anticancer effect of combined L-ascorbic acid (LAA) with superparamagnetic iron oxide nanoparticles (SPIONs) on adenocarcinoma gastric cell line (AGS). Fe3O4-LAA was synthesized using the reverse co-precipitation technique. XRD, FT-IR, FESEM, and TEM were employed to characterize the synthesized nanoparticles. MTT test and Real-time PCR were performed to investigate the cytotoxicity and apoptosis induced by treatments. The present study indicated that the anticancer properties of vitamin C were increased when employed as the Fe3O4-LAA nanoparticles. A dose-dependent manner on the effect of Fe3O4-LAA on investigated genes was observed; p53 gene expression changes in high and low doses of Fe3O4-LAA were 7.4 and 3.1 fold, respectively; accordingly, Bcl2 gene expression under treatment with high and low doses of Fe3O4-LAA was reduced by 71% and 25%, respectively. In conclusion, our results indicated that Fe3O4-LAA nanoparticles might be an efficient agent for cancer treatment.

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Targeting Cells With MR Imaging Probes: Cellular Interaction And Intracellular Magnetic Iron Oxide Nanoparticles Uptake In Brain Capillary Endothelial and Choroidal Plexus Epithelial Cells

10.1063/1.3505065 ◽

2010 ◽

Cited By ~ 1

Author(s):

I. Cambianica ◽

M. Bossi ◽

P. Gasco ◽

W. Gonzalez ◽

J. M. Idee ◽

...

Keyword(s):

Iron Oxide ◽

Epithelial Cells ◽

Mr Imaging ◽

Iron Oxide Nanoparticles ◽

Cellular Interaction ◽

Oxide Nanoparticles ◽

Magnetic Iron Oxide Nanoparticles ◽

Brain Capillary ◽

Magnetic Iron Oxide ◽

Imaging Probes

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Iron oxide nanoparticles induce cytokine secretion in a complement-dependent manner in a human whole blood model

International Journal of Nanomedicine ◽

10.2147/ijn.s136453 ◽

2017 ◽

Vol Volume 12 ◽

pp. 3927-3940 ◽

Cited By ~ 13

Author(s):

Susann Wolf-Grosse ◽

Anne Mari Rokstad ◽

Syed Ali ◽

John Lambris ◽

Tom Mollnes ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Whole Blood ◽

Cytokine Secretion ◽

Oxide Nanoparticles ◽

Dependent Manner ◽

Human Whole Blood

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Intraperitoneal exposure of iron oxide nanoparticles causes dose-dependent toxicity in Wistar rats

Toxicology and Industrial Health ◽

10.1177/07482337211058668 ◽

2021 ◽

pp. 074823372110586

Author(s):

Gajraj S Verma ◽

Naresh K Nirmal ◽

Deepika Gunpal ◽

Hemant Gupta ◽

Monika Yadav ◽

...

Keyword(s):

Body Weight ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Wistar Rats ◽

Serum Lactate Dehydrogenase ◽

Oxide Nanoparticles ◽

Mean Corpuscular Hemoglobin ◽

Dependent Manner ◽

Corpuscular Hemoglobin ◽

Control Serum

Nanoparticles of iron oxide, with diameters beteween 1 to 100 nm, have notable implications for human health and well being. In the current study, we have investigated the effects of iron oxide nanoparticles (IONP) exposure on general physiology and health of adult Wistar rats. IONP used in the study had spherical shape and average size in the range of 15–20 nm. A total of eight groups of rats were repeatedly injected with 0 (control), 20, 40, and 80 mg IONP per kg body weight intraperitoneally under two different exposure schemes (sub-acute and sub-chronic). IONP exposure caused significant changes in lungs, liver, and kidney indices in both exposure schemes. Sub-acute exposure did not affect body weight gain in treated rats, but longer duration exposure was responsible for significant reduction in body weight. Mesenteries, visceral fatty tissues, and visceral peritoneal membranes demonstrated apparent accumulations of IONP in a dose and time-dependent manner. Hematological analysis showed that total RBC count, hemoglobin content, hematocrit, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and mean platelet volume (MPV) were not affected by IONP exposure. Total lymphocyte count, however, was elevated in low- and mid-dose treated rats, but not in high-dose group. Serum lactate dehydrogenase (LDH) increased significantly in rats treated with mid and high doses as compared to control. Serum creatinine and blood urea nitrogen levels were also significantly altered in treated rats. Histological study found significant hepatic damage and mild spleen toxicity. Our report suggests that IONP exhibit significant toxicity in rats.

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