scholarly journals Extracellular matrix production by nucleus pulposus and bone marrow stem cells in response to altered oxygen and glucose microenvironments

2015 ◽  
Vol 227 (6) ◽  
pp. 757-766 ◽  
Author(s):  
Syeda M. Naqvi ◽  
Conor T. Buckley
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Nucleus Pulposus ◽  
Bone Marrow Stem Cells ◽  
Extracellular Matrix Production ◽  
Matrix Production

scholarly journals Laminin-5 activates extracellular matrix production and osteogenic gene focusing in human mesenchymal stem cells

2007 ◽  
Vol 26 (2) ◽  
pp. 106-114 ◽  
Author(s):  
Robert F. Klees ◽  
Roman M. Salasznyk ◽  
Scott Vandenberg ◽  
Kristin Bennett ◽  
George E. Plopper
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Laminin 5 ◽  
Extracellular Matrix Production ◽  
Matrix Production ◽  
Osteogenic Gene

scholarly journals Differentiation of cardiomyocytes from human embryonic stem cells is accompanied by changes in the extracellular matrix production of versican and hyaluronan

2010 ◽  
Vol 111 (3) ◽  
pp. 585-596 ◽  
Author(s):  
Christina K. Chan ◽  
Marsha W. Rolle ◽  
Susan Potter-Perigo ◽  
Kathleen R. Braun ◽  
Benjamin P. Van Biber ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Extracellular Matrix Production ◽  
Matrix Production

scholarly journals Bone marrow stem cells-derived extracellular matrix is a promising material

Oncotarget ◽  
2017 ◽  
Vol 8 (58) ◽  
pp. 98336-98347 ◽  
Author(s):  
Xiaoyan Wang ◽  
Guanghua Chen ◽  
Chao Huang ◽  
Hualei Tu ◽  
Jilong Zou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Bone Marrow Stem Cells ◽  
Promising Material

scholarly journals Hif-3α Fine-tunes Extracellular Matrix Production in Nucleus Pulposus Cell under Hypoxia

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582626-s-0036-1582626
Author(s):  
Wai-Kit Tam ◽  
Kenneth M. C. Cheung ◽  
Victor Y. L. Leung
Keyword(s):  
Extracellular Matrix ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cell ◽  
Extracellular Matrix Production ◽  
Matrix Production

Microenvironment of Apatite-Fiber Scaffold Affects Cell Proliferation and Resulting Cell Differentiation

2007 ◽  
Vol 361-363 ◽  
pp. 1075-1078
Author(s):  
Michiyo Honda ◽  
Shigeki Izumi ◽  
Nobuyuki Kanzawa ◽  
Takahide Tsuchiya ◽  
Mamoru Aizawa
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Bone Marrow Cells ◽  
Three Dimensional ◽  
Bone Marrow Stem Cells ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Appropriate culture conditions cause bone marrow stem cells to differentiate into multilineage cells such as adipocytes, chondrocytes, and osteoblasts. One key factor that regulates intercellular signaling and cell differentiation is the extracellular matrix microenvironment. The composition of the extracellular matrix influences cellular functions. In the present study, we investigated the effects of a microenvironment comprising a three-dimensional apatite-fiber scaffold (AFS) that has two kinds of pores (micro- and macro pores) on proliferation and subsequent differentiation of bone marrow stem cells. Morphologic observation revealed that osteoblastic cells in the AFS were distributed primarily in the same location on the fibrous scaffold and formed bridges within micro- and macro pores. We used molecular approaches to evaluate cell proliferation and differentiation in detail. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that culturing bone marrow cells on AFS increases expression of osteocalcin (OC) mRNA compared with culture in a dish. Furthermore, cells cultured in AFS expressed type X collagen (Col X), which is a marker of hypertrophic cartilage. These data suggest that the three-dimensional microenvironment of AFS facilitates cell proliferation and differentiation, and promotes endochondral ossification of bone marrow cells.

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