The effect of dermal fibroblasts and mesenchymal stem cells seeded on an amniotic membrane scaffold in skin regeneration: a case series

AIM: To evaluate therapeutic outcomes of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) treatment in patients with refractory uveitis. METHODS: A retrospective and noncomparative review was performed on four patients with refractory uveitis from December 2013 to December 2017. HUC-MSCs were administered intravenously at a dose of 1×106 cells/kg. Clinical response, relapse rate, change of visual acuity, and other metrics were evaluated. RESULTS: All four patients presented with responses to HUC-MSCs treatment, with three males and one female. The numbers of uveitis attacks per year after the HUC-MSCs treatment (0, 2, 0, 0 respectively) all decreased compared with the numbers before the treatment (3, 6, 4, 4 respectively). The oral steroid and immunosuppressive agents were tapered in all patients without recrudescence of ocular inflammation, and three patients discontinued their oral medicine at the last visit. The best corrected visual acuity (BCVA) of 3 patients was improved to varying degrees, and the BCVA of 1 patient remained at 20/20 (Snellen chart) from the first to the last consultation. CONCLUSION: The study provides an effective therapy of HUC-MSCs in maintaining remission in patients affected by uveitis refractory to previous immunosuppressant treatments.

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Canine amniotic membrane derived mesenchymal stem cells exosomes addition in canine sperm freezing medium

Journal of Animal Reproduction and Biotechnology ◽

10.12750/jarb.35.3.268 ◽

2020 ◽

Vol 35 (3) ◽

pp. 268-272

Author(s):

Feriel Yasmine Mahiddine ◽

Ahmad Yar Qamar ◽

Min Jung Kim

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Amniotic Membrane ◽

Freezing Medium ◽

Sperm Freezing

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Anti-aging effects of fetal dermal mesenchymal stem cells in a D-galactose-induced aging model of adult dermal fibroblasts

In Vitro Cellular & Developmental Biology - Animal ◽

10.1007/s11626-021-00624-z ◽

2021 ◽

Author(s):

Shengsheng Pan ◽

Siyu Gong ◽

Jingjuan Zhang ◽

Shanshan Jia ◽

Maoying Wang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Dermal Fibroblasts ◽

Aging Effects ◽

Aging Model

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Glutathione–Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair

International Journal of Molecular Sciences ◽

10.3390/ijms21051638 ◽

2020 ◽

Vol 21 (5) ◽

pp. 1638 ◽

Cited By ~ 1

Author(s):

Emilia Di Giovanni ◽

Silvia Buonvino ◽

Ivano Amelio ◽

Sonia Melino

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Tissue Repair ◽

Dermal Fibroblasts ◽

Water Soluble ◽

Dependent Manner ◽

Tissue Repair And Regeneration ◽

Stem Cell Delivery ◽

Cell Based Therapy

The endogenous gasotransmitter H2S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H2S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H2S-releasing agents on the growth of stem cells have not been fully investigated. H2S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H2S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H2S agents on the cell growth and differentiation of cardiac Lin− Sca1+ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH–garlic conjugates (GSGa) on cMSC compared to other H2S-releasing agents, such as Na2S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell–cell adhesion and commitment to differentiation. These results highlight the effects of H2S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.

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