Decreased expression of Kallmann syndrome 1 sequence gene (KAL1 ) contributes to oral squamous cell carcinoma progression and significantly correlates with poorly differentiated grade

2014 ◽  
Vol 44 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Jiannan Liu ◽  
Wei Cao ◽  
Wantao Chen ◽  
Liqun Xu ◽  
Chenping Zhang
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Kallmann Syndrome ◽  
Poorly Differentiated

scholarly journals Diagnostic and prognostic value of cancer stem cell marker CD44 and soluble CD44 in the peripheral Blood of patients with oral Squamous cell carcinoma

2017 ◽  
Vol 2 (3) ◽  
Author(s):  
Aya K. Khamis ◽  
Hamed A. Fouad ◽  
Hanaa S. Raslan ◽  
Mohamed M. Fata ◽  
Amira I. Fayad
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Stem Cell Marker ◽  
Soluble Cd44 ◽  
Poorly Differentiated ◽  
Diagnostic And Prognostic Value

BackgroundHead and neck cancer is a major health problem. Recent studies on the pathobiology of oral squamous cell carcinoma (OSCC) have led to the discovery of a small population of cancer cells with a consistent behavior with the features of cancer stem cells (CSCs).  CSCs are required and responsible for initiation, maintenance and recurrence of disease. Molecular markers are commonly used for the identification of CSCs. CD44 is the most reported CSC marker in OSCC.The aim of the study was to evaluate and correlate the expression of CD44 in different histopathological grades of OSCC, as well as to assess the diagnostic and prognostic value of soluble CD44 (CD44sol) in peripheral blood of patients.Materials and methodsFifteen patients with OSCC were included; biopsies were histologically evaluated using haematoxylin and eosin. Serial sections were immunohistochemically stained by monoclonal antibody to CD44. The intensity of immunostaining of CD44 was calculated. Enzyme-linked immunosorbent assay (ELISA) method was used to determine the concentration of CD44sol in the blood serum.ResultsAll grades of OSCC showed membranous immunosignaling of CD44. The well, moderately and poorly differentiated OSCC cases showed weak, moderate and intense positive membranous immunosignaling of CD44 respectively.CD44sol levels were significantly higher in OSCC patients than they were in control groups. Soluble CD44 serum levels were significantly higher in poorly differentiated than they were in moderately and well differentiated.ConclusionCSCs detection in fixed human tissue and CD44sol detection in peripheral blood using ELISA seemed to be a promising method and may have a diagnostic and prognostic value in management of OSCC.

scholarly journals ORAL SQUAMOUS CELL CARCINOMA

2015 ◽  
Vol 22 (01) ◽  
pp. 018-026
Author(s):  
Sofia Ali Syed ◽  
Mulazim Hussain Bukhari ◽  
Riaz Ahmed Warraich ◽  
Fauzia Quadir ◽  
Asifa Iqbal ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Maxillofacial Surgery ◽  
Nucleolar Organizer Regions ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Poorly Differentiated ◽  
Well Differentiated

Background: The aim of this study was to evaluate clinico-pathologicalparameters and the status of argyrophilic nucleolar organizer regions stain in various histologicalgrades of oral squamous cell carcinoma. Materials and Methods: A cross sectional studywas conducted on fifty cases of oral squamous cell carcinoma. The specimen were collectedfrom the department of Oral & Maxillofacial Surgery and processed for hematoxylin and eosinstain and AgNOR stain Pathology Laboratory, King Edward Medical University Lahore. Results:Bidi smoking is associated with oral squamous cell carcinoma. The AgNOR (mAgNOR andpAgNOR) status was significantly low in well differentiated and moderately differentiatedcompared to poorly differentiated oral squamous cell carcinoma (p =0.001). AgNOR size inpoorly differentiated was significantly higher than the AgNOR size in well differentiated oralsquamous cell carcinoma. Similarly the distribution of AgNOR in moderately and poorlydifferentiated oral squamous cell carcinoma was significantly high. The AgNORs index wassignificantly high in poorly differentiated squamous cell carcinoma as compared to welldifferentiated and moderately differentiated squamous cell carcinoma. Conclusions: The useof AgNORs stain is easy, valid and reliable method to assess the histological grading of oralsquamous cell carcinoma and should be used to predict the prognosis of patients.

scholarly journals Correlation of increased numbers of tumor-infiltrating T regulatory, CD19 and CD8 T cells in well differentiated oral squamous cell carcinoma

2018 ◽  
Vol 1 ◽  
pp. e201801004
Author(s):  
Victor Angelo MONTALLI ◽  
Fabrício PASSADOR-SANTOS ◽  
Andresa Borges SOARES ◽  
Elizabeth F. MARTINEZ ◽  
Denise B. R. RODRIGUES ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tumor Antigens ◽  
Tumor Grade ◽  
Inflammatory Cells ◽  
Tissue Microarrays ◽  
Poorly Differentiated ◽  
Well Differentiated

Evidence from cancer patients suggests that increases in T-regulatory lymphocyte (Treg) activity may be associated with poor immune responses against tumor antigens, and contribute to immune dysfunction. Since there have been contradictory reports concerning the physiological roles of the Treg and inflammatory cells in the tumor microenvironment, the inflammatory cell influx was investigated for different malignancygrades of oral squamous cell carcinoma (OSCC). A total of 42 patients with OSCC were selected. Tumors were histologically graded as well, moderately, or poorly differentiated. Tissue microarrays were performed to immunohistochemically measure the expression of CD8, Foxp3, CD19, and IL-10. Significant statistical increases (p<0.05) were observed in the numbers of Foxp3+ and CD8+ inflammatory cells, as well as inIL-10 expression, in the well differentiated tumors, compared to the poorly differentiated tumors. No differences between the three different histological grades were observed in the numbers of CD19+ cells. The relationship between the tumor grade and Treg and CD8 cell recruitment in patients with OSCC underlined the fact that the immune system is deregulated as a consequence of the disease.

scholarly journals Penentuan Grading Tumor Ganas Oral Squamous Cell Carcinoma Berdasarkan Gambaran Histopatologi

2015 ◽  
Vol 17 (1) ◽  
pp. 46
Author(s):  
Theresia Indah Budhy
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Stage Iv ◽  
Stage I ◽  
Stage Iii ◽  
Low Grade ◽  
Poorly Differentiated ◽  
Well Differentiated

AbstrakLatar Belakang: Sekitar 95% dari tumor ganas diklasifikassikan secara histologis sebagai Oral Squamous Cell Carcinoma (OSCC). Secara mikroskopis OSCC diklasifikasikan berdasarkan pada metode yang mempehitungkan penilaian subjektif, tingkat keratinisasi, pleomorfik nukleus dan seluler, dan aktivitas mitosis. Tingkatan tersebut meliputi well differentiated (grade I-II), moderately differentiated (grade III), dan poorly differentiated (grade IV). Well dan moderately differentiated dapat dikelompokkan sebagai low grade sedangkan poorly differentiated tumor sebagai high grade. Standar yang paling baik untuk menegakkan diagnosis OSCC adalah pemeriksaan histopatologis dan biopsi jaringan lesi tersebut. Tujuan: Penelitian ini bertujuan untuk menentukan grading tumor ganas OSCC berdasarkan gambaran histopatologi. Metode: Sampel terdiri dari 6 jenis OSCC kemudian dilakukan pembuatan sediaan jaringan tumor ganas OSCC kedalam blok paraffin dan dilakukan pengecatan menggunakan hematoxilin eosin (HE). Gambaran histopatologi dari keenam sampel diamati dibawah mikroskop cahaya dengan pembesaran 100x dan 400x.   Hasil: Dari 6 kasus yang ditemukan satu terdiagnosa sebagai well differentiated (stage I), dua kasus terdiagnosa well differentiated(sstage II),  dua kasus terdiagnosa moderately differentiated (stage III), dan poorly differentiated (stage IV). Kesimpulan: OSCC diklasifikasikan berdasarkan gambaran histopatologi kedalam well differentiated (stage I-II), moderately differentiated (stage III), dan poorly differentiated (stage IV)Kata Kunci : OSCC, well differentiated, moderately differentiated, poorly differentiated

scholarly journals Evaluation of Podoplanin in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Ashok Patil ◽  
Kishor Patil ◽  
Suyog Tupsakhare ◽  
Mahesh Gabhane ◽  
Shrikant Sonune ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Oral Leukoplakia ◽  
Severe Dysplasia ◽  
Podoplanin Expression ◽  
Poorly Differentiated ◽  
One Way Anova

Background. Recent studies have demonstrated that podoplanin was expressed in some dysplastic lesions adjacent to primary oral cancers suggesting that podoplanin expression may occur in early oral tumorigenesis and lymphangiogenesis and therefore is related to tumor growth. The purpose of this study is to determine the role of podoplanin as a biomarker for cancer risk assessment in oral leukoplakia and correlation of podoplanin expression with grades of oral squamous cell carcinoma (OSCC).Materials and Methods. In the present retrospective study, podoplanin expression was investigated immunohistochemically in 40 patients each of oral leukoplakia and OSCC. The scores were analyzed statistically using one-way ANOVA test followed by Tukey HSD.Results. By applying one-way ANOVA test, there was a highly significant increase of the podoplanin expression from mild to severe dysplasia and from well to poorly differentiated OSCC (P<0.01). Statistically highly significant difference was present between scores of mild to moderate dysplasia, moderate to severe dysplasia, well to poorly differentiated OSCC, and moderately to poorly differentiated OSCC (Tukey HSD test,P<0.01).Conclusion. Podoplanin can be used as a biomarker for early oral tumorigenesis and for malignant transformation risk assessment of premalignant lesions and as a tumor progression biomarker for advanced grades of OSCC.

scholarly journals Prognostic Implications of ALDH1 and Notch1 in Different Subtypes of Oral Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Silas Antonio Juvencio de Freitas Filho ◽  
Cláudia Malheiros Coutinho-Camillo ◽  
Katia Klug Oliveira ◽  
Bárbara Beltrame Bettim ◽  
Clóvis Antônio Lopes Pinto ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Basaloid Squamous Cell Carcinoma ◽  
Poorly Differentiated ◽  
Basaloid Squamous

Background. The present study aimed to investigate the clinical significance and prognostic value of the immunoexpression of cancer stem cell markers, ALDH1 and Notch1, in subtypes of oral squamous cell carcinoma. Materials and Methods. The expression of ALDH1 and Notch1 in 63 patients with well and poorly differentiated oral squamous cell carcinomas and their subtypes, verrucous carcinoma and basaloid squamous cell carcinoma, was evaluated by immunohistochemistry. The semi-quantitative analysis of the ALDH1 and Notch immunoexpression levels, based on the capture of 10 microscopic fields, at 400X magnification, at the invasive tumor front was performed and associated with clinicopathological variables using the chi-square test or Fisher’s exact test. The overall and disease-free survival rates were estimated according to the Kaplan–Meier method and the curves were compared using the log-rank test. The independent effects of variables were calculated using Cox’s proportional hazards regression model. Results. Strong ALDH1 and Notch1 expression was observed in 16 (25.4%) and 27 (42.9%) oral squamous cell carcinomas including their subtypes, respectively. Most tumors with strong immunoexpression of ALDH1 were basaloid squamous cell carcinoma (56.3%). Statistically significant associations were observed between the strong immunoexpression of Notch1 in poorly differentiated oral squamous cell carcinoma with perineural infiltration ( p  = 0.011) and lymph node involvement (pN+) ( p  = 0.034). The strong immunoexpression of ALDH1 was a prognostic factor associated with worse overall survival ( p  = 0.040) for patients with oral cancer. Conclusion. The strong immunoexpression of Notch1 can contribute to identification of patients with poorly differentiated oral squamous cell carcinoma, who have perineural infiltration or lymph node metastasis. In addition, the strong immunoexpression of ALDH1 may help to identify a worse prognosis in patients with oral squamous cell carcinoma and their subtypes.

Prognostic value of cathepsin L and its inhibitor headpin in oral squamous cell carcinoma

2012 ◽  
Vol 126 (11) ◽  
pp. 1134-1137 ◽  
Author(s):  
T Nakashima ◽  
R Yasumatsu ◽  
M Masuda ◽  
G L Clayman ◽  
S Komune
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cathepsin L ◽  
Prognostic Significance ◽  
Metastatic Potential ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Immunohistochemical Studies

AbstractObjective:To investigate the clinicopathological and prognostic significance of the expression of cathepsin L and its inhibitor headpin, in oral squamous cell carcinoma.Design:Immunohistochemical studies were performed on 56 oral squamous cell carcinoma samples. We evaluated the relationship between cathepsin L and headpin expression versus patients' clinicopathological factors and survival.Results:The group that was positive for cathepsin L expression tended to have positive metastatic neck lymph nodes and a poorer prognosis. Headpin expression was not related to metastasis or prognosis. Well differentiated squamous cell carcinoma had higher levels of headpin expression compared with poorly differentiated squamous cell carcinoma.Conclusion:Cathepsin L expression is related to the invasive and metastatic potential of oral squamous cell carcinoma.

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