scholarly journals Correlation of increased numbers of tumor-infiltrating T regulatory, CD19 and CD8 T cells in well differentiated oral squamous cell carcinoma

2018 ◽  
Vol 1 ◽  
pp. e201801004
Author(s):  
Victor Angelo MONTALLI ◽  
Fabrício PASSADOR-SANTOS ◽  
Andresa Borges SOARES ◽  
Elizabeth F. MARTINEZ ◽  
Denise B. R. RODRIGUES ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tumor Antigens ◽  
Tumor Grade ◽  
Inflammatory Cells ◽  
Tissue Microarrays ◽  
Poorly Differentiated ◽  
Well Differentiated

Evidence from cancer patients suggests that increases in T-regulatory lymphocyte (Treg) activity may be associated with poor immune responses against tumor antigens, and contribute to immune dysfunction. Since there have been contradictory reports concerning the physiological roles of the Treg and inflammatory cells in the tumor microenvironment, the inflammatory cell influx was investigated for different malignancygrades of oral squamous cell carcinoma (OSCC). A total of 42 patients with OSCC were selected. Tumors were histologically graded as well, moderately, or poorly differentiated. Tissue microarrays were performed to immunohistochemically measure the expression of CD8, Foxp3, CD19, and IL-10. Significant statistical increases (p<0.05) were observed in the numbers of Foxp3+ and CD8+ inflammatory cells, as well as inIL-10 expression, in the well differentiated tumors, compared to the poorly differentiated tumors. No differences between the three different histological grades were observed in the numbers of CD19+ cells. The relationship between the tumor grade and Treg and CD8 cell recruitment in patients with OSCC underlined the fact that the immune system is deregulated as a consequence of the disease.

scholarly journals ORAL SQUAMOUS CELL CARCINOMA

2015 ◽  
Vol 22 (01) ◽  
pp. 018-026
Author(s):  
Sofia Ali Syed ◽  
Mulazim Hussain Bukhari ◽  
Riaz Ahmed Warraich ◽  
Fauzia Quadir ◽  
Asifa Iqbal ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Maxillofacial Surgery ◽  
Nucleolar Organizer Regions ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Poorly Differentiated ◽  
Well Differentiated

Background: The aim of this study was to evaluate clinico-pathologicalparameters and the status of argyrophilic nucleolar organizer regions stain in various histologicalgrades of oral squamous cell carcinoma. Materials and Methods: A cross sectional studywas conducted on fifty cases of oral squamous cell carcinoma. The specimen were collectedfrom the department of Oral & Maxillofacial Surgery and processed for hematoxylin and eosinstain and AgNOR stain Pathology Laboratory, King Edward Medical University Lahore. Results:Bidi smoking is associated with oral squamous cell carcinoma. The AgNOR (mAgNOR andpAgNOR) status was significantly low in well differentiated and moderately differentiatedcompared to poorly differentiated oral squamous cell carcinoma (p =0.001). AgNOR size inpoorly differentiated was significantly higher than the AgNOR size in well differentiated oralsquamous cell carcinoma. Similarly the distribution of AgNOR in moderately and poorlydifferentiated oral squamous cell carcinoma was significantly high. The AgNORs index wassignificantly high in poorly differentiated squamous cell carcinoma as compared to welldifferentiated and moderately differentiated squamous cell carcinoma. Conclusions: The useof AgNORs stain is easy, valid and reliable method to assess the histological grading of oralsquamous cell carcinoma and should be used to predict the prognosis of patients.

scholarly journals Penentuan Grading Tumor Ganas Oral Squamous Cell Carcinoma Berdasarkan Gambaran Histopatologi

2015 ◽  
Vol 17 (1) ◽  
pp. 46
Author(s):  
Theresia Indah Budhy
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Stage Iv ◽  
Stage I ◽  
Stage Iii ◽  
Low Grade ◽  
Poorly Differentiated ◽  
Well Differentiated

AbstrakLatar Belakang: Sekitar 95% dari tumor ganas diklasifikassikan secara histologis sebagai Oral Squamous Cell Carcinoma (OSCC). Secara mikroskopis OSCC diklasifikasikan berdasarkan pada metode yang mempehitungkan penilaian subjektif, tingkat keratinisasi, pleomorfik nukleus dan seluler, dan aktivitas mitosis. Tingkatan tersebut meliputi well differentiated (grade I-II), moderately differentiated (grade III), dan poorly differentiated (grade IV). Well dan moderately differentiated dapat dikelompokkan sebagai low grade sedangkan poorly differentiated tumor sebagai high grade. Standar yang paling baik untuk menegakkan diagnosis OSCC adalah pemeriksaan histopatologis dan biopsi jaringan lesi tersebut. Tujuan: Penelitian ini bertujuan untuk menentukan grading tumor ganas OSCC berdasarkan gambaran histopatologi. Metode: Sampel terdiri dari 6 jenis OSCC kemudian dilakukan pembuatan sediaan jaringan tumor ganas OSCC kedalam blok paraffin dan dilakukan pengecatan menggunakan hematoxilin eosin (HE). Gambaran histopatologi dari keenam sampel diamati dibawah mikroskop cahaya dengan pembesaran 100x dan 400x.   Hasil: Dari 6 kasus yang ditemukan satu terdiagnosa sebagai well differentiated (stage I), dua kasus terdiagnosa well differentiated(sstage II),  dua kasus terdiagnosa moderately differentiated (stage III), dan poorly differentiated (stage IV). Kesimpulan: OSCC diklasifikasikan berdasarkan gambaran histopatologi kedalam well differentiated (stage I-II), moderately differentiated (stage III), dan poorly differentiated (stage IV)Kata Kunci : OSCC, well differentiated, moderately differentiated, poorly differentiated

Prognostic value of cathepsin L and its inhibitor headpin in oral squamous cell carcinoma

2012 ◽  
Vol 126 (11) ◽  
pp. 1134-1137 ◽  
Author(s):  
T Nakashima ◽  
R Yasumatsu ◽  
M Masuda ◽  
G L Clayman ◽  
S Komune
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cathepsin L ◽  
Prognostic Significance ◽  
Metastatic Potential ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Immunohistochemical Studies

AbstractObjective:To investigate the clinicopathological and prognostic significance of the expression of cathepsin L and its inhibitor headpin, in oral squamous cell carcinoma.Design:Immunohistochemical studies were performed on 56 oral squamous cell carcinoma samples. We evaluated the relationship between cathepsin L and headpin expression versus patients' clinicopathological factors and survival.Results:The group that was positive for cathepsin L expression tended to have positive metastatic neck lymph nodes and a poorer prognosis. Headpin expression was not related to metastasis or prognosis. Well differentiated squamous cell carcinoma had higher levels of headpin expression compared with poorly differentiated squamous cell carcinoma.Conclusion:Cathepsin L expression is related to the invasive and metastatic potential of oral squamous cell carcinoma.

Immunohistochemical Expression of TGF-β3 in Oral Squamous Cell Carcinoma

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Asmaa Ali Hussein
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Transforming Growth Factor ◽  
Tumor Grade ◽  
Tumor Stage ◽  
High Rate ◽  
Female Ratio ◽  
Positive Expression

Squamous cell carcinoma characterized by poor prognosis due to aggressive tumor growth and dissemination high rate of tumor cell . age ranged of patient case included in the study 40-62 years and mean age 55±99. The sex distribution male/female ratio 1:1. Male case 15 and female 15 of the present study The results of clinical forums showed in the current study was endophytic 10(33.3%) in the same time Exophytic were presented in 20 cases (76.7%). Regarding distribution of the tumors site, the preponderance of them 19 cases 73.3% were located alveolar mucosa, followed by in the tongue 11 cases(36.7%) Tumor stage was analyzed and recorded in Oral squamous cell carcinoma included cases, the preponderance of them were Stage II 11 cases 36.7% followed by stage III 10 cases 33.3% , 9 cases 30.0% were stage I. While Concerning tumor grade, majority of them 15 cases 50% had grade II moderately differentiated SCC, while 11 cases 36.7% had grade III poorly differentiated SCC and 4 cases 13.3% had grade I well differentiated SCC Positive TGF-β3 immunostaining was detected as cell with staining brown color, all tissues sections included show Positive expression based on IHC teqnique. Positive Transforming Growth Factor TGF-β3 Immuno staining was found in all case results and display that 4 samples with percentage 13.3% expressed strong positive 87.67 ± 1.45 expression , 11cases 36.7% showed 51.33 ±0.88 positive expression moderate at the same time 15 samples 50.0% showed positive weak expression.

scholarly journals Cutaneous squamous cell carcinoma arising in hidradenitis suppurativa: A case report

2019 ◽  
Vol 7 ◽  
pp. 2050313X1984735 ◽  
Author(s):  
Catherine F Roy ◽  
Simon F Roy ◽  
Feras M Ghazawi ◽  
Erica Patocskai ◽  
Annie Bélisle ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Hidradenitis Suppurativa ◽  
Rare Complication ◽  
Excisional Biopsy ◽  
Necrosis Factor Alpha ◽  
Spindle Cells ◽  
Poorly Differentiated ◽  
Well Differentiated

We present a case of a 64-year-old man who presented with a rapidly growing tumor in the left buttock and intergluteal cleft area, which was affected by hidradenitis suppurativa. The patient was on tumor necrosis factor-alpha inhibitors for hidradenitis suppurativa for 2 years prior to the development of the mass. Initial biopsy of the mass showed a well-differentiated squamous cell carcinoma with spindle cells and positive epithelial immunomarkers. Subsequent excisional biopsy of the tumor showed an infiltrating poorly differentiated squamous cell carcinoma composed of islands of atypical sarcomatoid spindle cells. Squamous cell carcinoma arising in hidradenitis suppurativa is a rare complication which may occur secondary to chronic inflammation and epidermal hyperproliferation in hidradenitis suppurativa–affected areas.

scholarly journals Diagnostic and prognostic value of cancer stem cell marker CD44 and soluble CD44 in the peripheral Blood of patients with oral Squamous cell carcinoma

2017 ◽  
Vol 2 (3) ◽  
Author(s):  
Aya K. Khamis ◽  
Hamed A. Fouad ◽  
Hanaa S. Raslan ◽  
Mohamed M. Fata ◽  
Amira I. Fayad
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Stem Cell Marker ◽  
Soluble Cd44 ◽  
Poorly Differentiated ◽  
Diagnostic And Prognostic Value

BackgroundHead and neck cancer is a major health problem. Recent studies on the pathobiology of oral squamous cell carcinoma (OSCC) have led to the discovery of a small population of cancer cells with a consistent behavior with the features of cancer stem cells (CSCs).  CSCs are required and responsible for initiation, maintenance and recurrence of disease. Molecular markers are commonly used for the identification of CSCs. CD44 is the most reported CSC marker in OSCC.The aim of the study was to evaluate and correlate the expression of CD44 in different histopathological grades of OSCC, as well as to assess the diagnostic and prognostic value of soluble CD44 (CD44sol) in peripheral blood of patients.Materials and methodsFifteen patients with OSCC were included; biopsies were histologically evaluated using haematoxylin and eosin. Serial sections were immunohistochemically stained by monoclonal antibody to CD44. The intensity of immunostaining of CD44 was calculated. Enzyme-linked immunosorbent assay (ELISA) method was used to determine the concentration of CD44sol in the blood serum.ResultsAll grades of OSCC showed membranous immunosignaling of CD44. The well, moderately and poorly differentiated OSCC cases showed weak, moderate and intense positive membranous immunosignaling of CD44 respectively.CD44sol levels were significantly higher in OSCC patients than they were in control groups. Soluble CD44 serum levels were significantly higher in poorly differentiated than they were in moderately and well differentiated.ConclusionCSCs detection in fixed human tissue and CD44sol detection in peripheral blood using ELISA seemed to be a promising method and may have a diagnostic and prognostic value in management of OSCC.

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