Fine‐scale genetic diversity and putative ecotypes of oxymonad protists co‐inhabiting the hindgut of Reticulitermes speratus

2021 ◽  
Author(s):  
Katsura Igai ◽  
Osamu Kitade ◽  
Jieyang Fu ◽  
Kazumi Omata ◽  
Takahiro Yonezawa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Fine Scale ◽  
Reticulitermes Speratus

scholarly journals Population Genetic Structure and Habitat Connectivity for Jaguar (Panthera onca) Conservation in Central Belize.

2019 ◽  
Author(s):  
Angelica Menchaca ◽  
Natalia Rossi ◽  
Jeremy Froidevaux ◽  
Isabela Dias-freedman ◽  
Anthony Caragiulo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Genetic Structure ◽  
Genetic Connectivity ◽  
Suitable Habitat ◽  
Fine Scale ◽  
Panthera Onca ◽  
Wildlife Sanctuary ◽  
Landscape Permeability ◽  
Forest Reserve

Abstract Connectivity among jaguar (Panthera onca) populations will ensure natural gene flow and the long-term survival of the species throughout its range. Jaguar conservation efforts have focused primarily on connecting suitable habitat in a broad-scale. Accelerated habitat reduction, human-wildlife conflict, limited funding, and the complexity of jaguar behaviour have proven challenging to maintain connectivity between populations effectively. Here, we used non-invasive genetic sampling and individual-based conservation genetic analyses to assess genetic diversity and levels of genetic connectivity between individuals in the Cockscomb Basin Wildlife Sanctuary and the Maya Forest Corridor. We used expert knowledge and scientific literature to develop models of landscape permeability based on circuit theory with fine-scale landscape features as ecosystem types, distance to human settlements and roads to predict the most probable jaguar movement across central Belize. Results We used 12 highly polymorphic microsatellite loci to identify 50 individual jaguars. We detected high levels of genetic diversity across loci (HE= 0.61, HO= 0.55, and NA=9.33). Using Bayesian clustering and multivariate models to assess gene flow and genetic structure, we identified one single group of jaguars (K = 1). We identified critical areas for jaguar movement that fall outside the boundaries of current protected areas in central Belize. We detected two main areas of high landscape permeability in a stretch of approximately 18 km between Sittee River Forest Reserve and Manatee Forest Reserve that may increase functional connectivity and facilitate jaguar dispersal from and to Cockscomb Basin Wildlife Sanctuary. Our analysis provides important insights on fine-scale genetic and landscape connectivity of jaguars in central Belize, an area of conservation concern. Conclusions The results of our study demonstrate high levels of relatively recent gene flow for jaguars between two study sites in central Belize. Our landscape analysis detected corridors of expected jaguar movement between the Cockscomb Basin Wildlife Sanctuary and the Maya Forest Corridor. We highlight the importance of maintaining already established corridors and consolidating new areas that further promote jaguar movement across suitable habitat beyond the boundaries of currently protected areas. Continued conservation efforts within identified corridors will further maintain and increase genetic connectivity in central Belize.

scholarly journals Patterns of Genetic Diversity and Structure at Fine Scale of an Endangered Moroccan Endemic Tree (Argania spinosa L. Skeels) Based on ISSR Polymorphism

2015 ◽  
Vol 43 (2) ◽  
Author(s):  
Jamila MOUHADDAB ◽  
Naima AIT AABD ◽  
Hafid ACHTAK ◽  
Fouad MSANDA ◽  
Abdelaziz ZAHIDI ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Fine Scale ◽  
Argania Spinosa ◽  
Genetic Diversity And Structure

scholarly journals Fine-Scale Population Genetic Structure and Within-Tree Distribution of Mating Types of Venturia effusa, Cause of Pecan Scab in the United States

2018 ◽  
Vol 108 (11) ◽  
pp. 1326-1336 ◽  
Author(s):  
Clive H. Bock ◽  
Carolyn A. Young ◽  
Katherine L. Stevenson ◽  
Nikki D. Charlton
Keyword(s):  
United States ◽  
Genetic Diversity ◽  
Asexual Reproduction ◽  
Population Genetic ◽  
Spatial Scales ◽  
The United States ◽  
Source Population ◽  
Mating Types ◽  
Fine Scale ◽  
Scale Population

Scab (caused by Venturia effusa) is the major disease of pecan in the southeastern United States. There is no information available on the fine-scale population genetic diversity or the occurrence of clonal types at small spatial scales that provides insight into inoculum sources and dispersal mechanisms, and potential opportunity for sexual reproduction. To investigate fine-scale genetic diversity, four trees of cultivar Wichita (populations) were sampled hierarchically: within each tree canopy, four approximately evenly spaced terminals (subpopulations) were selected and up to six leaflets (sub-subpopulations) were sampled from different compound leaves on each terminal. All lesions (n = 1 to 8) on each leaflet were sampled. The isolates were screened against a panel of 29 informative microsatellite markers and the resulting multilocus genotypes (MLG) subject to analysis. Mating type was also determined for each isolate. Of 335 isolates, there were 165 MLG (clonal fraction 49.3%). Nei’s unbiased measure of genetic diversity for the clone-corrected data were moderate to high (0.507). An analysis of molecular variance demonstrated differentiation (P = 0.001) between populations on leaflets within individual terminals and between terminals within trees in the tree canopies, with 93.8% of variance explained among isolates within leaflet populations. Other analyses (minimum-spanning network, Bayesian, and discriminant analysis of principal components) all indicated little affinity of isolate for source population. Of the 335 isolates, most unique MLG were found at the stratum of the individual leaflets (n = 242), with similar total numbers of unique MLG observed at the strata of the terminal (n = 170), tree (n = 166), and orchard (n = 165). Thus, the vast majority of shared clones existed on individual leaflets on a terminal at the scale of 10s of centimeters or less, indicating a notable component of short-distance dispersal. There was significant linkage disequilibrium (P < 0.001), and an analysis of Psex showed that where there were multiple encounters of an MLG, they were most probably the result of asexual reproduction (P < 0.05) but there was no evidence that asexual reproduction was involved in single or first encounters of an MLG (P > 0.05). Overall, the MAT1-1-1 and MAT1-2-1 idiomorphs were at equilibrium (73:92) and in most populations, subpopulations, and sub-subpopulations. Both mating types were frequently observed on the same leaflet. The results provide novel information on the characteristics of populations of V. effusa at fine spatial scales, and provide insights into the dispersal of the organism within and between trees. The proximity of both mating idiomorphs on single leaflets is further evidence of opportunity for development of the sexual stage in the field.

Fine-scale genetic diversity and landscape-scale genetic structuring in three foundational bulrush species: implications for wetland revegetation

2018 ◽  
Vol 27 (2) ◽  
pp. 408-420 ◽  
Author(s):  
Karin M. Kettenring ◽  
Bret N. Mossman ◽  
Rebekah Downard ◽  
Karen E. Mock
Keyword(s):  
Genetic Diversity ◽  
Landscape Scale ◽  
Fine Scale ◽  
Genetic Structuring

scholarly journals Applying new inter-individual approaches to assess fine-scale population genetic diversity in a neotropical frog, Eleutherodactylus ockendeni

Heredity ◽  
2007 ◽  
Vol 99 (5) ◽  
pp. 506-515 ◽  
Author(s):  
K R Elmer ◽  
J A Dávila ◽  
S C Lougheed
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Fine Scale ◽  
Population Genetic Diversity ◽  
Scale Population

scholarly journals Population Genetic Structure and Habitat Connectivity for Jaguar (Panthera onca) Conservation in Central Belize.

2019 ◽  
Author(s):  
Angelica Menchaca ◽  
Natalia Rossi ◽  
Jeremy Froidevaux ◽  
Isabela Dias-freedman ◽  
Anthony Caragiulo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Genetic Structure ◽  
Genetic Connectivity ◽  
Suitable Habitat ◽  
Fine Scale ◽  
Panthera Onca ◽  
Wildlife Sanctuary ◽  
Landscape Permeability ◽  
Forest Reserve

Abstract Background Connectivity among jaguar (Panthera onca) populations will ensure natural gene flow and the long-term survival of the species throughout its range. Jaguar conservation efforts have focused primarily on connecting suitable habitat in a broad-scale. Accelerated habitat reduction, human-wildlife conflict, limited funding, and the complexity of jaguar behaviour have proven challenging to maintain connectivity between populations effectively. Here, we used non-invasive genetic sampling and individual-based conservation genetic analyses to assess genetic diversity and levels of genetic connectivity between individuals in the Cockscomb Basin Wildlife Sanctuary and the Maya Forest Corridor. We used expert knowledge and scientific literature to develop models of landscape permeability based on circuit theory with fine-scale landscape features as ecosystem types, distance to human settlements and roads to predict the most probable jaguar movement across central Belize. Results We used 12 highly polymorphic microsatellite loci to identify 50 individual jaguars. We detected high levels of genetic diversity across loci (HE= 0.61, HO= 0.55, and NA=9.33). Using Bayesian clustering and multivariate models to assess gene flow and genetic structure, we identified one single group of jaguars (K = 1). We identified critical areas for jaguar movement that fall outside the boundaries of current protected areas in central Belize. We detected two main areas of high landscape permeability in a stretch of approximately 18 km between Sittee River Forest Reserve and Manatee Forest Reserve that may increase functional connectivity and facilitate jaguar dispersal from and to Cockscomb Basin Wildlife Sanctuary. Our analysis provides important insights on fine-scale genetic and landscape connectivity of jaguars in central Belize, an area of conservation concern. Conclusions The results of our study demonstrate high levels of relatively recent gene flow for jaguars between two study sites in central Belize. Our landscape analysis detected corridors of expected jaguar movement between the Cockscomb Basin Wildlife Sanctuary and the Maya Forest Corridor. We highlight the importance of maintaining already established corridors and consolidating new areas that further promote jaguar movement across suitable habitat beyond the boundaries of currently protected areas. Continued conservation efforts within identified corridors will further maintain and increase genetic connectivity in central Belize.

scholarly journals Broad-scale puma connectivity could restore genomic diversity to fine-scale coastal populations

2021 ◽  
Author(s):  
Kyle D Gustafson ◽  
Roderick B Gagne ◽  
Michael R Buchalski ◽  
T Winston Vickers ◽  
Seth PD Riley ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Linkage Disequilibrium ◽  
Puma Concolor ◽  
Genomic Diversity ◽  
Strong Linkage Disequilibrium ◽  
Fine Scale ◽  
High Genetic Diversity ◽  
Population Genomic ◽  
Genome Wide ◽  
Genetic Clusters

Urbanization is decreasing wildlife habitat and connectivity worldwide, including for apex predators, such as the puma (Puma concolor). Puma populations along California's central and southern coastal habitats have experienced rapid fragmentation from development, leading to calls for demographic and genetic management. To address urgent conservation genomic concerns, we used double-digest restriction-site associated DNA (ddRAD) sequencing to analyze 16,285 genome-wide single-nucleotide polymorphisms (SNPs) from 401 broadly sampled pumas. Our analyses indicated support for 4–10 geographically nested, broad- to fine-scale genetic clusters. At the broadest scale, the 4 genetic clusters had high genetic diversity and exhibited low linkage disequilibrium, indicating pumas have retained statewide genomic diversity. However, multiple lines of evidence indicated substructure, including 10 fine-scale genetic clusters, some of which exhibited allelic fixation and linkage disequilibrium. Fragmented populations along the Southern Coast and Central Coast had particularly low genetic diversity and strong linkage disequilibrium, indicating genetic drift and close inbreeding. Our results demonstrate that genetically at-risk populations are typically nested within a broader-scale group of interconnected populations that collectively retains high genetic diversity and heterogeneous fixations. Thus, extant variation at the broader scale has potential to restore diversity to local populations if management actions can enhance vital gene flow and recombine locally sequestered genetic diversity. These state- and genome-wide results are critically important for science-based conservation and management practices. Our broad- and fine-scale population genomic analysis highlights the information that can be gained from population genomic studies aiming to provide guidance for fragmented population conservation management.

Fine-scale genetic diversity and relatedness in fungi associated with the mountain pine beetle

2019 ◽  
Vol 49 (8) ◽  
pp. 933-941 ◽  
Author(s):  
Clement K.-M. Tsui ◽  
Stéphanie Beauseigle ◽  
Dario I. Ojeda Alayon ◽  
Adrianne V. Rice ◽  
Janice E.K. Cooke ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mountain Pine Beetle ◽  
Spatial Genetic Structure ◽  
Fungal Species ◽  
Fine Scale ◽  
Symbiotic Associations ◽  
Mountain Pine ◽  
Pine Beetle ◽  
Sexual Recombination ◽  
Ophiostoma Montium

The mountain pine beetle (MPB; Dendroctonus ponderosae Hopkins, 1902) forms beneficial symbiotic associations with fungi. Here we explored the fine-scale spatial genetic structure of three of those fungi using single nucleotide polymorphism. We found that single mated pairs of beetles carry not only multiple fungal species, but also multiple genotypes of each species into their galleries. We observed genetic diversity at a fine spatial scale. Most of the diversity was found within and among galleries with nonsignificant diversity among trees. We observed clonal propagation almost exclusively within galleries. Ophiostoma montium (Rumbold) Arx possessed a larger expected number of multilocus genotypes and lower linkage disequilibrium than Grosmannia clavigera (Rob.-Jeffr. & R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. and Leptographium longiclavatum S.W. Lee, J.J. Kim & C. Breuil. More than 80% of fungal samples were genetically unrelated, a result that parallels what has been observed in the beetles. The proportion of genetically related samples within galleries was higher in O. montium (40%) than in G. clavigera (20%) or L. longiclavatum (6%), likely the consequence of within-gallery sexual recombination in O. montium. The underlying genetic diversity reported here and the differences among fungal species could enable the symbiont community to quickly respond to new environmental conditions or changes in the host, enhancing the maintenance of this multipartite relationship and allowing the MPB to colonize new habitats.

scholarly journals Influence of Forest Road Culverts and Waterfalls on the Fine-Scale Distribution of Brook Trout Genetic Diversity in a Boreal Watershed

2014 ◽  
Vol 143 (6) ◽  
pp. 1577-1591 ◽  
Author(s):  
Jean-Baptiste Torterotot ◽  
Charles Perrier ◽  
Normand E. Bergeron ◽  
Louis Bernatchez
Keyword(s):  
Genetic Diversity ◽  
Brook Trout ◽  
Fine Scale ◽  
Forest Road
Sign in / Sign up

Export Citation Format

Share Document