scholarly journals Cauliflower mosaic virus protein P6‐TAV plays a major role in alteration of aphid vector feeding behaviour but not performance on infected Arabidopsis

2021 ◽  
Author(s):  
Quentin Chesnais ◽  
Maxime Verdier ◽  
Myriam Burckbuchler ◽  
Véronique Brault ◽  
Mikhail Pooggin ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Feeding Behaviour ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein ◽  
Aphid Vector

Epidemiology of Cauliflower Mosaic virus in South Australia

1967 ◽  
Vol 18 (2) ◽  
pp. 289 ◽  
Author(s):  
JW Randles ◽  
NC Crowley
Keyword(s):  
Mosaic Virus ◽  
South Australia ◽  
Brevicoryne Brassicae ◽  
Cauliflower Mosaic Virus ◽  
Vector Species ◽  
Aphid Vector ◽  
Brassica Crops ◽  
Significant Spread

Results are presented of 3 years of aphid trapping and 3 years of surveys of the incidence of infection with cauliflower mosaic virus in Brassica crops at two sites in South Australia. Mosaic infection has been found to be of importance only in cauliflower plantings. The incidence of the disease varied greatly at the two sites, which were located in climatically different regions. In the Adelaide Hills significant spread of the disease occurred only through the autumn months March, April, and May, and in only one of the three years did the incidence of the disease reach epidemic proportions. On the Adelaide Plains spread of the disease occurred throughout the cooler months of the year, from March till September. At both locations increasing incidence of the disease followed increases in the number of trapped aphid vector species. Results indicate that the most important vector in the field is likely to be Brevicoryne brassicae. Trials suggest that seed-bed infection with the virus can be reduced by the use of barrier crops.

scholarly journals The Cauliflower Mosaic Virus Protein P6 Forms Motile Inclusions That Traffic along Actin Microfilaments and Stabilize Microtubules

2008 ◽  
Vol 149 (2) ◽  
pp. 1005-1016 ◽  
Author(s):  
Phillip A. Harries ◽  
Karuppaiah Palanichelvam ◽  
Weichang Yu ◽  
James E. Schoelz ◽  
Richard S. Nelson
Keyword(s):  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein ◽  
Actin Microfilaments

scholarly journals Identification of the domains of cauliflower mosaic virus protein P6 responsible for suppression of RNA silencing and salicylic acid signalling

2013 ◽  
Vol 94 (12) ◽  
pp. 2777-2789 ◽  
Author(s):  
Janet Laird ◽  
Carol McInally ◽  
Craig Carr ◽  
Sowjanya Doddiah ◽  
Gary Yates ◽  
...  
Keyword(s):  
Cell Death ◽  
Salicylic Acid ◽  
Mosaic Virus ◽  
Virus Replication ◽  
Nuclear Export ◽  
Rna Silencing ◽  
Nuclear Export Signal ◽  
Genetically Modified Crops ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein

Cauliflower mosaic virus (CaMV) encodes a 520 aa polypeptide, P6, which participates in several essential activities in the virus life cycle including suppressing RNA silencing and salicylic acid-responsive defence signalling. We infected Arabidopsis with CaMV mutants containing short in-frame deletions within the P6 ORF. A deletion in the distal end of domain D-I (the N-terminal 112 aa) of P6 did not affect virus replication but compromised symptom development and curtailed the ability to restore GFP fluorescence in a GFP-silenced transgenic Arabidopsis line. A deletion in the minimum transactivator domain was defective in virus replication but retained the capacity to suppress RNA silencing locally. Symptom expression in CaMV-infected plants is apparently linked to the ability to suppress RNA silencing. When transiently co-expressed with tomato bushy stunt virus P19, an elicitor of programmed cell death in Nicotiana tabacum, WT P6 suppressed the hypersensitive response, but three mutants, two with deletions within the distal end of domain D-I and one involving the N-terminal nuclear export signal (NES), were unable to do so. Deleting the N-terminal 20 aa also abolished the suppression of pathogen-associated molecular pattern-dependent PR1a expression following agroinfiltration. However, the two other deletions in domain D-I retained this activity, evidence that the mechanisms underlying these functions are not identical. The D-I domain of P6 when expressed alone failed to suppress either cell death or PR1a expression and is therefore necessary but not sufficient for all three defence suppression activities. Consequently, concerns about the biosafety of genetically modified crops carrying truncated ORFVI sequences appear unfounded.

scholarly journals Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector

2002 ◽  
Vol 99 (4) ◽  
pp. 2422-2427 ◽  
Author(s):  
M. Drucker ◽  
R. Froissart ◽  
E. Hebrard ◽  
M. Uzest ◽  
M. Ravallec ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Intracellular Distribution ◽  
Cauliflower Mosaic Virus ◽  
Viral Gene ◽  
Gene Products ◽  
Complex Mechanism ◽  
Aphid Vector ◽  
Virus Acquisition

scholarly journals The Composition of Cauliflower Mosaic Virus Protein

1975 ◽  
Vol 27 (1) ◽  
pp. 101-106 ◽  
Author(s):  
A. A. Brunt ◽  
R. J. Barton ◽  
J. H. Tremaine ◽  
R. Stace-Smith
Keyword(s):  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein

scholarly journals Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Valentina Turri ◽  
Olga S. Latinovic ◽  
Massimiliano Bonafè ◽  
Ngeh Toyang ◽  
Maria Parigi ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Plant Virus ◽  
Sequence Similarity ◽  
Genetic Material ◽  
Glioma Cells ◽  
Human Cells ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein ◽  
Human Beings

Recent comparisons between plant and animal viruses reveal many common principles that underlie how all viruses express their genetic material, amplify their genomes, and link virion assembly with replication. Cauliflower mosaic virus (CaMV) is not infectious for human beings. Here, we show that CaMV transactivator/viroplasmin protein (TAV) shares sequence similarity with and behaves like the human ribonuclease H1 (RNase H1) in reducing DNA/RNA hybrids detected with S9.6 antibody in HEK293T cells. We showed that TAV is clearly expressed in the cytosol and in the nuclei of transiently transfected human cells, similar to its distribution in plants. TAV also showed remarkable cytotoxic effects in U251 human glioma cells in vitro. These characteristics pave the way for future analysis on the use of the plant virus protein TAV, as an alternative to human RNAse H1 during gene therapy in human cells.

scholarly journals Cauliflower mosaic virus Protein P6 Inhibits Signaling Responses to Salicylic Acid and Regulates Innate Immunity

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47535 ◽  
Author(s):  
Andrew J. Love ◽  
Chiara Geri ◽  
Janet Laird ◽  
Craig Carr ◽  
Byung-Wook Yun ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Salicylic Acid ◽  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein

scholarly journals Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing

2007 ◽  
Vol 88 (12) ◽  
pp. 3439-3444 ◽  
Author(s):  
Andrew J. Love ◽  
Janet Laird ◽  
Justin Holt ◽  
Andrew J. Hamilton ◽  
Ari Sadanandom ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Symptom Severity ◽  
Rna Silencing ◽  
Transgenic Arabidopsis ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein ◽  
Silencing Suppressor ◽  
Northern Blots ◽  
Gfp Fluorescence ◽  
Suppressor Of Rna Silencing

We infected a transgenic Arabidopsis line (GxA), containing an amplicon-silenced 35S : : GFP transgene, with cauliflower mosaic virus (CaMV), a plant pararetrovirus with a DNA genome. Systemically infected leaves showed strong GFP fluorescence and amplicon transcripts were detectable in Northern blots, indicating that silencing of GFP had been suppressed during CaMV-infection. Transgenic Arabidopsis lines expressing CaMV protein P6, the major genetic determinant of symptom severity, were crossed with GxA. Progeny showed strong GFP fluorescence throughout and amplicon transcripts were detectable in Northern blots, indicating that P6 was suppressing local and systemic silencing. However, levels of 21 nt siRNAs derived from the GFP transgene were not reduced. In CaMV-infected plants, the P6 transgene did not reduce levels of CaMV leader-derived 21 and 24 nt siRNAs relative to levels of CaMV 35S RNA. These results demonstrate that CaMV can efficiently suppress silencing of a GFP transgene, and that P6 acts as a silencing suppressor.

scholarly journals Differences in the mechanism of inoculation between a semi-persistent and a non-persistent aphid-transmitted plant virus

2012 ◽  
Vol 93 (3) ◽  
pp. 662-667 ◽  
Author(s):  
Aranzazu Moreno ◽  
W. Freddy Tjallingii ◽  
Gabriela Fernandez-Mata ◽  
Alberto Fereres
Keyword(s):  
Mosaic Virus ◽  
Aphid Species ◽  
Cauliflower Mosaic Virus ◽  
Test Plant ◽  
Electrical Penetration Graph ◽  
Mesophyll Cells ◽  
Aphid Vector ◽  
Persistent Viruses ◽  
Epg Technique

Inoculation of the semi-persistent cauliflower mosaic virus (CaMV, genus Caulimovirus) is associated with successive brief (5–10 s) intracellular stylet punctures (pd) when aphids probe in epidermal and mesophyll cells. In contrast to non-persistent viruses, there is no evidence for which of the pd subphases (II-1, II-2 and II-3) is involved in the inoculation of CaMV. Experiments were conducted using the electrical penetration graph (EPG) technique to investigate which particular subphases of the pd are associated with the inoculation of CaMV to turnip by its aphid vector Brevicoryne brassicae. In addition, the same aphid species/test plant combination was used to compare the role of the pd subphases in the inoculation of the non-persistent turnip mosaic virus (TuMV, genus Potyvirus). Inoculation of TuMV was found to be related to subphase II-1, confirming earlier results, but CaMV inoculation appeared to be related exclusively to subphase II-2 instead. The mechanism of CaMV inoculation and the possible nature of subphase II-2 are discussed in the scope of our findings.

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