scholarly journals Sex‐biased gene expression in dioecious garden asparagus ( Asparagus officinalis )

2015 ◽  
Vol 207 (3) ◽  
pp. 883-892 ◽  
Author(s):  
Alex Harkess ◽  
Francesco Mercati ◽  
Hong‐Yan Shan ◽  
Francesco Sunseri ◽  
Agostino Falavigna ◽  
...  
Keyword(s):  
Gene Expression ◽  
Asparagus Officinalis ◽  
Garden Asparagus

Controlled atmospheres and sugar can delay malate synthase gene expression during asparagus senescence

2002 ◽  
Vol 29 (9) ◽  
pp. 1045 ◽  
Author(s):  
Simon A. Coupe ◽  
Ben K. Sinclair ◽  
Sheryl D. Somerfield ◽  
Paul L. Hurst
Keyword(s):  
Gene Expression ◽  
Single Gene ◽  
Malate Synthase ◽  
Open Reading Frame ◽  
Asparagus Officinalis ◽  
High Identity ◽  
Reading Frame ◽  
Controlled Atmospheres ◽  
Foliar Senescence

A cDNA clone encoding malate synthase (MS; EC 4.1.3.2) was isolated from a 48-h postharvest asparagus (Asparagus officinalis L.) spear cDNA library using a MS clone from Brassica napus. The asparagus MS (AoMS1) cDNA hybridized to a 1.9-kb transcript that increased in abundance preferentially in spear-tip tissue during postharvest storage. The AoMS1 transcript also accumulated during natural foliar senescence of asparagus fern. The cDNA consists of 1960 nucleotides with an open reading frame of 1665 nucleotides or 555 amino acids, and encodes a deduced protein with a predicted Mr of 63 kDa and a pI of 8.1. The deduced amino acid sequence of AoMS1 showed high identity with the B. napus MS clone (77.2%) used to isolate it, and with MS from cucumber (77%). Genomic Southern analysis suggests that a single gene in asparagus encodes AoMS1. Controlled- atmosphere treatments aimed at reducing deterioration of harvested asparagus spears reduced the expression of AoMS1. The reduction was correlated with the reduced oxygen level, and reduced MS enzyme activity was also observed. Asparagus cell cultures were used to test the role of sugar status in regulating AoMS1 gene expression. In cultures without sucrose there was an accumulation of AoMS1 transcript that was absent in cultures containing sucrose.

Self- and cross-incompatibility in Asparagus officinalis and Asparagus densiflorus cv. Sprengeri

1996 ◽  
Vol 74 (10) ◽  
pp. 1621-1625 ◽  
Author(s):  
O. N. Marcellán ◽  
E. L. Camadro
Keyword(s):  
Fluorescence Microscopy ◽  
Reproductive Isolation ◽  
Pollen Tubes ◽  
Asparagus Officinalis ◽  
Valuable Source ◽  
Stigmatic Surface ◽  
Commercial Cultivars ◽  
Garden Asparagus ◽  
Internal Barriers ◽  
Incompatibility Reactions

Garden asparagus, Asparagus officinalis L. (off), is dioecious and reproductively isolated from a related ornamental, monoecious Asparagus densifiorus (Kunth) Jessop cv. Sprengeri (spr). Since the latter is a potentially valuable source of germplasm, a study was initiated to identify hybridization barriers. Intra- and inter-specific crosses were made using 32 plants of two commercial cultivars of off and 17 plants of three introductions of spr. Part of the pollinated pistils were fixed and examined via fluorescence microscopy. In some combinations of genotypes, incompatibility reactions were detected: (i) off × off: in the stigmatic tissue, (ii) spr × spr: on the stigmatic surface and in the style, and (iii) spr × off: on the stigmatic surface, in the stigmatic tissue, and in the style. Although pollen tubes reached the ovules in most combinations of genotypes, seeds were only produced in intraspecific crosses. It is concluded that two types of internal barriers are acting: cross-incompatibility at the pollen–stigma and pollen–style levels, and stronger post-stylar barriers that had not been determined yet. Keywords: Asparagus densifiorus cv. Sprengeri, Asparagus officinalis, cross-incompatibility, reproductive isolation.

scholarly journals TGIF-DB: terse genomics interface for developing botany

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Daisuke Tsugama ◽  
Tetsuo Takano
Keyword(s):  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Asparagus Officinalis ◽  
Tartary Buckwheat ◽  
Whole Genome Sequence ◽  
Fagopyrum Tataricum ◽  
Crop Species ◽  
Putative Gene ◽  
Functional Annotations ◽  
Garden Asparagus

Abstract Objectives Pearl millet (Pennisetum glaucum) is a staple cereal crop for semi-arid regions. Its whole genome sequence and deduced putative gene sequences are available. However, the functions of many pearl millet genes are unknown. Situations are similar for other crop species such as garden asparagus (Asparagus officinalis), chickpea (Cicer arietinum) and Tartary buckwheat (Fagopyrum tataricum). The objective of the data presented here was to improve functional annotations of genes of pearl millet, garden asparagus, chickpea and Tartary buckwheat with gene annotations of model plants, to systematically provide such annotations as well as their sequences on a website, and thereby to promote genomics for those crops. Data description Sequences of genomes and transcripts of pearl millet, garden asparagus, chickpea and Tartary buckwheat were downloaded from a public database. These transcripts were associated with functional annotations of their Arabidopsis thaliana and rice (Oryza sativa) counterparts identified by BLASTX. Conserved domains in protein sequences of those species were identified by the HMMER scan with the Pfam database. The resulting data was deposited in the figshare repository and can be browsed on the Terse Genomics Interface for Developing Botany (TGIF-DB) website (http://webpark2116.sakura.ne.jp/rlgpr/).

scholarly journals Development of insect-resistant cotton lines with targeted expression of insecticidal gene

2016 ◽  
Vol 68 (4) ◽  
pp. 773-780 ◽  
Author(s):  
Allah Bakhsh ◽  
Emine Anayol ◽  
Saber Khabbazi ◽  
Ömer Karakoç ◽  
Cengiz Sancak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Constitutive Expression ◽  
Inducible Promoter ◽  
Asparagus Officinalis ◽  
Foreign Gene ◽  
Nptii Gene ◽  
Mechanical Wounding ◽  
Pathogenesis Related Protein ◽  
Primary Transformants ◽  
Insect Resistant

In order to address biosafety concerns regarding the constitutive expression of foreign genes in crops, we applied a strategy aimed at confining foreign gene expression in insect wounding sites of cotton. For this purpose, a plant expression construct was designed by cloning the AoPR1 promoter (pathogenesis-related protein gene isolated from Asparagus officinalis) upstream from the insecticidal gene cry1Ac. The Turkish cotton cultivar cv. STN-468 was transformed using the Agrobacterium tumefaciens strain LBA4404 containing the recombinant binary vector pRD400 harboring cry1Ac under a wound-inducible promoter. The neomycin phosphotransferase (nptII) gene was used as a selectable marker at a concentration of 100 mg/L. The primary transformants were analyzed for T-DNA integration and expression using standard molecular approaches. The efficacy of insecticidal gene control of the AoPR1 promoter was investigated using leaf bioassays with 2nd instar larvae of Helicoverpa armigera and Spodoptera littoralis. Positive primary transformants from T0 progeny were further raised under greenhouse conditions to obtain progeny (T1). The introduced gene was properly inherited and expressed in T1 progeny. The mechanical wounding of plants resulted in increased cry1Ac protein levels during 0-48 h of the wounding period. The transgenic lines exhibited appreciable levels of resistance against targeted insect pests in the leaf bioassays. The use of a wound-inducible promoter to drive insecticidal gene expression is a valuable insect resistant management strategy as gene expression will remain limited to the insect biting sites of plant and crop, food and environmental concerns can be minimized.

scholarly journals Sex chromosome evolution via two genes

2018 ◽  
Author(s):  
Alex Harkess ◽  
Kun Huang ◽  
Ron van der Hulst ◽  
Bart Tissen ◽  
Jeffrey L Caplan ◽  
...  
Keyword(s):  
Single Molecule ◽  
Sex Chromosomes ◽  
Sex Chromosome ◽  
Male Gametophyte ◽  
Asparagus Officinalis ◽  
Female Function ◽  
Y Chromosomes ◽  
Autosome Pair ◽  
Garden Asparagus ◽  
And Function

The origin of sex chromosomes has been hypothesized to involve the linkage of factors with antagonistic effects on male and female function. Garden asparagus (Asparagus officinalis L.) is an ideal species to test this hypothesis, as the X and Y chromosomes are cytologically homomorphic and recently evolved from an ancestral autosome pair in association with a shift from hermaphroditism to dioecy. Mutagenesis screens paired with single-molecule fluorescence in situ hybridization (smFISH) directly implicate Y-specific genes that respectively suppress female organ development and are necessary for male gametophyte development. Comparison of contiguous X and Y chromosome shows that loss of recombination between the genes suppressing female function (SUPPRESSOR OF FEMALE FUNCTION, SOFF) and promoting male function (TAPETAL DEVELOPMENT AND FUNCTION 1, aspTDF1) is due to hemizygosity. We also experimentally demonstrate the function of aspTDF1. These finding provide direct evidence that sex chromosomes can evolve from autosomes via two sex determination genes: a dominant suppressor of femaleness and a promoter of maleness.

Single nucleotide polymorphism isolated from a novel EST dataset in garden asparagus (Asparagus officinalis L.)

Plant Science ◽  
2013 ◽  
Vol 203-204 ◽  
pp. 115-123 ◽  
Author(s):  
Francesco Mercati ◽  
Paolo Riccardi ◽  
Jim Leebens-Mack ◽  
Maria Rosa Abenavoli ◽  
Agostino Falavigna ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Asparagus Officinalis ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Garden Asparagus

Comparative genomic analyses in Asparagus

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 1052-1060 ◽  
Author(s):  
Joseph C Kuhl ◽  
Michael J Havey ◽  
William J Martin ◽  
Foo Cheung ◽  
Qiaoping Yuan ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Gc Content ◽  
Asparagus Officinalis ◽  
Comparative Genomic ◽  
African Species ◽  
Genome Doubling ◽  
Coding Regions ◽  
The Core ◽  
Garden Asparagus ◽  
Acorus Americanus

Garden asparagus (Asparagus officinalis L.) belongs to the monocot family Asparagaceae in the order Asparagales. Onion (Allium cepa L.) and Asparagus officinalis are 2 of the most economically important plants of the core Asparagales, a well supported monophyletic group within the Asparagales. Coding regions in onion have lower GC contents than the grasses. We compared the GC content of 3374 unique expressed sequence tags (ESTs) from A. officinalis with Lycoris longituba and onion (both members of the core Asparagales), Acorus americanus (sister to all other monocots), the grasses, and Arabidopsis. Although ESTs in A. officinalis and Acorus had a higher average GC content than Arabidopsis, Lycoris, and onion, all were clearly lower than the grasses. The Asparagaceae have the smallest nuclear genomes among all plants in the core Asparagales, which typically have huge genomes. Within the Asparagaceae, European Asparagus species have approximately twice the nuclear DNA of that of southern African Asparagus species. We cloned and sequenced 20 genomic amplicons from European A. officinalis and the southern African species Asparagus plumosus and observed no clear evidence for a recent genome doubling in A. officinalis relative to A. plumosus. These results indicate that members of the genus Asparagus with smaller genomes may be useful genomic models for plants in the core Asparagales.Key words: asparagus, GC content, duplication.

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