Transcriptomic and Physiological Analyses Reveal the Dynamic Response to Salinity Stress of the Garden Asparagus (Asparagus officinalis L.)

Garden asparagus, Asparagus officinalis L. (off), is dioecious and reproductively isolated from a related ornamental, monoecious Asparagus densifiorus (Kunth) Jessop cv. Sprengeri (spr). Since the latter is a potentially valuable source of germplasm, a study was initiated to identify hybridization barriers. Intra- and inter-specific crosses were made using 32 plants of two commercial cultivars of off and 17 plants of three introductions of spr. Part of the pollinated pistils were fixed and examined via fluorescence microscopy. In some combinations of genotypes, incompatibility reactions were detected: (i) off × off: in the stigmatic tissue, (ii) spr × spr: on the stigmatic surface and in the style, and (iii) spr × off: on the stigmatic surface, in the stigmatic tissue, and in the style. Although pollen tubes reached the ovules in most combinations of genotypes, seeds were only produced in intraspecific crosses. It is concluded that two types of internal barriers are acting: cross-incompatibility at the pollen–stigma and pollen–style levels, and stronger post-stylar barriers that had not been determined yet. Keywords: Asparagus densifiorus cv. Sprengeri, Asparagus officinalis, cross-incompatibility, reproductive isolation.

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Data of whole genome sequencing of five garden asparagus (Asparagus officinalis) individuals with the MinION nanopore sequencer

Data in Brief ◽

10.1016/j.dib.2019.104838 ◽

2020 ◽

Vol 28 ◽

pp. 104838

Author(s):

Daisuke Tsugama ◽

Kaien Fujino

Keyword(s):

Whole Genome Sequencing ◽

Genome Sequencing ◽

Asparagus Officinalis ◽

Whole Genome ◽

Garden Asparagus ◽

Nanopore Sequencer

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TGIF-DB: terse genomics interface for developing botany

BMC Research Notes ◽

10.1186/s13104-021-05599-4 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Daisuke Tsugama ◽

Tetsuo Takano

Keyword(s):

Pearl Millet ◽

Pennisetum Glaucum ◽

Asparagus Officinalis ◽

Tartary Buckwheat ◽

Whole Genome Sequence ◽

Fagopyrum Tataricum ◽

Crop Species ◽

Putative Gene ◽

Functional Annotations ◽

Garden Asparagus

Abstract Objectives Pearl millet (Pennisetum glaucum) is a staple cereal crop for semi-arid regions. Its whole genome sequence and deduced putative gene sequences are available. However, the functions of many pearl millet genes are unknown. Situations are similar for other crop species such as garden asparagus (Asparagus officinalis), chickpea (Cicer arietinum) and Tartary buckwheat (Fagopyrum tataricum). The objective of the data presented here was to improve functional annotations of genes of pearl millet, garden asparagus, chickpea and Tartary buckwheat with gene annotations of model plants, to systematically provide such annotations as well as their sequences on a website, and thereby to promote genomics for those crops. Data description Sequences of genomes and transcripts of pearl millet, garden asparagus, chickpea and Tartary buckwheat were downloaded from a public database. These transcripts were associated with functional annotations of their Arabidopsis thaliana and rice (Oryza sativa) counterparts identified by BLASTX. Conserved domains in protein sequences of those species were identified by the HMMER scan with the Pfam database. The resulting data was deposited in the figshare repository and can be browsed on the Terse Genomics Interface for Developing Botany (TGIF-DB) website (http://webpark2116.sakura.ne.jp/rlgpr/).

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Sex chromosome evolution via two genes

10.1101/494112 ◽

2018 ◽

Author(s):

Alex Harkess ◽

Kun Huang ◽

Ron van der Hulst ◽

Bart Tissen ◽

Jeffrey L Caplan ◽

...

Keyword(s):

Single Molecule ◽

Sex Chromosomes ◽

Sex Chromosome ◽

Male Gametophyte ◽

Asparagus Officinalis ◽

Female Function ◽

Y Chromosomes ◽

Autosome Pair ◽

Garden Asparagus ◽

And Function

The origin of sex chromosomes has been hypothesized to involve the linkage of factors with antagonistic effects on male and female function. Garden asparagus (Asparagus officinalis L.) is an ideal species to test this hypothesis, as the X and Y chromosomes are cytologically homomorphic and recently evolved from an ancestral autosome pair in association with a shift from hermaphroditism to dioecy. Mutagenesis screens paired with single-molecule fluorescence in situ hybridization (smFISH) directly implicate Y-specific genes that respectively suppress female organ development and are necessary for male gametophyte development. Comparison of contiguous X and Y chromosome shows that loss of recombination between the genes suppressing female function (SUPPRESSOR OF FEMALE FUNCTION, SOFF) and promoting male function (TAPETAL DEVELOPMENT AND FUNCTION 1, aspTDF1) is due to hemizygosity. We also experimentally demonstrate the function of aspTDF1. These finding provide direct evidence that sex chromosomes can evolve from autosomes via two sex determination genes: a dominant suppressor of femaleness and a promoter of maleness.

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Single nucleotide polymorphism isolated from a novel EST dataset in garden asparagus (Asparagus officinalis L.)

Plant Science ◽

10.1016/j.plantsci.2013.01.002 ◽

2013 ◽

Vol 203-204 ◽

pp. 115-123 ◽

Cited By ~ 18

Author(s):

Francesco Mercati ◽

Paolo Riccardi ◽

Jim Leebens-Mack ◽

Maria Rosa Abenavoli ◽

Agostino Falavigna ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Asparagus Officinalis ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Garden Asparagus

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Comparative genomic analyses in Asparagus

Genome ◽

10.1139/g05-073 ◽

2005 ◽

Vol 48 (6) ◽

pp. 1052-1060 ◽

Cited By ~ 18

Author(s):

Joseph C Kuhl ◽

Michael J Havey ◽

William J Martin ◽

Foo Cheung ◽

Qiaoping Yuan ◽

...

Keyword(s):

Nuclear Dna ◽

Gc Content ◽

Asparagus Officinalis ◽

Comparative Genomic ◽

African Species ◽

Genome Doubling ◽

Coding Regions ◽

The Core ◽

Garden Asparagus ◽

Acorus Americanus

Garden asparagus (Asparagus officinalis L.) belongs to the monocot family Asparagaceae in the order Asparagales. Onion (Allium cepa L.) and Asparagus officinalis are 2 of the most economically important plants of the core Asparagales, a well supported monophyletic group within the Asparagales. Coding regions in onion have lower GC contents than the grasses. We compared the GC content of 3374 unique expressed sequence tags (ESTs) from A. officinalis with Lycoris longituba and onion (both members of the core Asparagales), Acorus americanus (sister to all other monocots), the grasses, and Arabidopsis. Although ESTs in A. officinalis and Acorus had a higher average GC content than Arabidopsis, Lycoris, and onion, all were clearly lower than the grasses. The Asparagaceae have the smallest nuclear genomes among all plants in the core Asparagales, which typically have huge genomes. Within the Asparagaceae, European Asparagus species have approximately twice the nuclear DNA of that of southern African Asparagus species. We cloned and sequenced 20 genomic amplicons from European A. officinalis and the southern African species Asparagus plumosus and observed no clear evidence for a recent genome doubling in A. officinalis relative to A. plumosus. These results indicate that members of the genus Asparagus with smaller genomes may be useful genomic models for plants in the core Asparagales.Key words: asparagus, GC content, duplication.

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