Characterization of a novel arsenite long‐distance transporter from arsenic hyperaccumulator fern Pteris vittata

2022 ◽  
Author(s):  
Huili Yan ◽  
Wenxiu Xu ◽  
Tian Zhang ◽  
Lu Feng ◽  
Ruoxi Liu ◽  
...  
2010 ◽  
Vol 56 (3) ◽  
pp. 236-246 ◽  
Author(s):  
Anhui Huang ◽  
Max Teplitski ◽  
Bala Rathinasabapathi ◽  
Lena Ma

Arsenic hyperaccumulator fern Pteris vittata L. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. Total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/L of arsenate showed a diverse arsenate-resistant microbial community from the rhizosphere of P. vittata growing in arsenic-contaminated sites. Twelve bacterial isolates tolerating 400 mmol/L of arsenate in liquid culture were identified. Selected bacterial isolates belonging to different genera were tested for their resistance to osmotic and oxidative stresses. Results showed that growth was generally better under osmotic stress generated by arsenic than under that generated by NaCl or PEG 6000, demonstrating that arsenic detoxification metabolism also cross-protected bacterial isolates from arsenic-induced osmotic stress. After 32 h of growth, all arsenate at 1 mmol/L was reduced to arsenite by strains Naxibacter sp. AH4, Mesorhizobium sp. AH5, and Pseudomonas sp. AH21, but arsenite at 1 mmol/L remained unchanged. Sensitivity to hydrogen peroxide was similar to that in broad-host pathogen Salmonella enterica sv. Typhimurium wild type, except strain AH4. The results suggested that these arsenic-resistant bacteria are metabolically adapted to arsenic-induced osmotic or oxidative stresses in addition to the specific bacterial system to exclude cellular arsenic. Both these adaptations contribute to the high arsenic resistance in the bacterial isolates.


1994 ◽  
Vol 269 (35) ◽  
pp. 22178-22187
Author(s):  
E.R. Groupp ◽  
N. Crawford ◽  
J. Locker

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yi Huang-Takeshi Kohda ◽  
Zhaojie Qian ◽  
Mei-Fang Chien ◽  
Keisuke Miyauchi ◽  
Ginro Endo ◽  
...  

AbstractPteris vittata is an arsenic (As) hyperaccumulator plant that accumulates a large amount of As into fronds and rhizomes (around 16,000 mg/kg in both after 16 weeks hydroponic cultivation with 30 mg/L arsenate). However, the sequence of long-distance transport of As in this hyperaccumulator plant is unclear. In this study, we used a positron-emitting tracer imaging system (PETIS) for the first time to obtain noninvasive serial images of As behavior in living plants with positron-emitting 74As-labeled tracer. We found that As kept accumulating in rhizomes as in fronds of P. vittata, whereas As was retained in roots of a non-accumulator plant Arabidopsis thaliana. Autoradiograph results of As distribution in P. vittata showed that with low As exposure, As was predominantly accumulated in young fronds and the midrib and rachis of mature fronds. Under high As exposure, As accumulation shifted from young fronds to mature fronds, especially in the margin of pinna, which resulted in necrotic symptoms, turning the marginal color to gray and then brown. Our results indicated that the function of rhizomes in P. vittata was As accumulation and the regulation of As translocation to the mature fronds to protect the young fronds under high As exposure.


2017 ◽  
Vol 69 (7) ◽  
pp. 463-478 ◽  
Author(s):  
Sara Pardal ◽  
Anna Drews ◽  
José A. Alves ◽  
Jaime A. Ramos ◽  
Helena Westerdahl
Keyword(s):  
Class I ◽  

2012 ◽  
Vol 15 (2) ◽  
pp. 187-216 ◽  
Author(s):  
Mercedes Murillo-Barroso ◽  
Marcos Martinón-Torres

The use of amber is documented in the Iberian peninsula since the Palaeolithic. The procurement and trade of this fossil resin has often been considered in discussions of long-distance trade and the emergence of social complexity, but so far no comprehensive view of the Iberian evidence has been produced to allow a more overarching interpretive model. This paper presents the Fourier-transform infrared spectroscopy (FTIR) characterization of archaeological amber from three Iberian prehistoric sites: a necklace recovered from the megalithic site of Palacio III (Almadén de la Plata, Sevilla), a pommel from PP4 Montelirio (Valencina de la Concepción, Sevilla), and a necklace from the Muricecs de Cellers cave (Llimiana, Pallars Jussà, Lleida). Based on these new data and a review of the literature, we present an overview that outlines fluctuations in the use of amber since the Upper Palaeolithic and demonstrates long-distance amber exchange connecting Iberia with northern Europe and the Mediterranean region since the Chalcolithic period at least. We discuss changes in the origins and cultural use of amber and their implications for the consolidation of trade networks.


Author(s):  
Zhibin Dou ◽  
Zenghua Zhao ◽  
Quan Jin ◽  
Gaotao Shi ◽  
Lianfang Zhang ◽  
...  
Keyword(s):  

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