Mapping ofRe, a gene conferring the red leaf trait in ornamental kale (Brassica oleraceaL. var.acephala)

Abstract Background Leaf shape is an important agronomic trait in ornamental kale (Brassica oleracea L. var. acephala). Although some leaf shape-related genes have been reported in ornamental kale, the detailed mechanism underlying leaf shape formation is still unclear. Here, we report a lobed-leaf trait in ornamental kale, aiming to analyze its inheritance and identify the strong candidate gene. Results Genetic analysis of F2 and BC1 populations demonstrate that the lobed-leaf trait in ornamental kale is controlled by a single dominant gene, termed BoLl-1 (Brassica oleracea lobed-leaf). By performing whole-genome resequencing and linkage analyses, the BoLl-1 gene was finely mapped to a 127-kb interval on chromosome C09 flanked by SNP markers SL4 and SL6, with genetic distances of 0.6 cM and 0.6 cM, respectively. Based on annotations of the genes within this interval, Bo9g181710, an orthologous gene of LATE MERISTEM IDENTITY 1 (LMI1) in Arabidopsis, was predicted as the candidate for BoLl-1, and was renamed BoLMI1a. The expression level of BoLMI1a in lobed-leaf parent 18Q2513 was significantly higher compared with unlobed-leaf parent 18Q2515. Sequence analysis of the parental alleles revealed no sequence variations in the coding sequence of BoLMI1a, whereas a 1737-bp deletion, a 92-bp insertion and an SNP were identified within the BoLMI1a promoter region of parent 18Q2513. Verification analyses with BoLMI1a-specific markers corresponding to the promoter variations revealed that the variations were present only in the lobed-leaf ornamental kale inbred lines. Conclusions This study identified a lobed-leaf gene BoLMI1a, which was fine-mapped to a 127-kb fragment. Three variations were identified in the promoter region of BoLMI1a. The transcription level of BoLMI1a between the two parents exhibited great difference, providing new insight into the molecular mechanism underlying leaf shape formation in ornamental kale.

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Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala)

Genetics and Molecular Research ◽

10.4238/2013.april.2.3 ◽

2013 ◽

Vol 12 (2) ◽

pp. 870-877 ◽

Cited By ~ 5

Author(s):

Y.S. Wang ◽

Z.Y. Liu ◽

Y.F. Li ◽

Y. Zhang ◽

X.F. Yang ◽

...

Keyword(s):

Brassica Oleracea ◽

Leaf Trait ◽

Ornamental Kale ◽

Brassica Oleracea L

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Promoter Variations Strongly Enhance the Transcription Level of the BoLMI1 Gene, Causing Lobed Leaves in Ornamental Kale (Brassica Oleracea L. Var. Acephala)

10.21203/rs.3.rs-512886/v1 ◽

2021 ◽

Author(s):

Bin Zhang ◽

Wendi Chen ◽

Xing Li ◽

Wenjing Ren ◽

Li Chen ◽

...

Keyword(s):

Brassica Oleracea ◽

Shape Change ◽

Leaf Shape ◽

Dominant Gene ◽

Snp Markers ◽

Transcription Level ◽

Leaf Trait ◽

Shape Formation ◽

Ornamental Kale ◽

Lobed Leaf

Abstract Background: Leaf shape is an important agronomic trait in ornamental kale (Brassica oleracea L. var. acephala). Although some leaf shape-related genes have been reported in ornamental kale, the detailed mechanism underlying leaf shape formation is still unclear. Here, we report a lobed-leaf trait in ornamental kale, aiming to analyze its inheritance and identify the strong candidate gene.Results: Genetic analysis of F2 and BC1 populations demonstrate that the lobed-leaf trait in ornamental kale is controlled by a single dominant gene, termed BoLl-1. By performing whole-genome resequencing and linkage analyses, the BoLl-1 gene was finely mapped to a 127-kb interval on chromosome C09 flanked by SNP markers SL4 and SL6, with genetic distances of 0.6 cM and 0.6 cM, respectively. Based on annotations of the genes within this interval, Bo9g181710, an orthologous gene of LATE MERISTEM IDENTITY 1 (LMI1) in Arabidopsis, was predicted as the candidate for BoLl-1, and was renamed BoLMI1. The expression level of BoLMI1 in lobed-leaf parent 18Q2513 was significantly higher compared with unlobed-leaf parent 18Q2515. Sequence analysis of the parental alleles revealed no sequence variations in the coding sequence of BoLMI1, whereas a 1737-bp deletion, a 92-bp insertion and an SNP were identified within the BoLMI1 promoter region of parent 18Q2513. Verification analyses with BoLMI1-specific markers corresponding to the promoter variations revealed that the variations were present only in the lobed-leaf ornamental kale inbred lines.Conclusions: This study demonstrated that promoter variations strongly enhance the transcription level of BoLMI1 and cause the leaf shape change from unlobed to lobed, providing new insight into the molecular mechanism underlying leaf shape formation in ornamental kale.

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Leaf Trait-Environment Relationships in a Subtropical Broadleaved Forest in South-East China

PLoS ONE ◽

10.1371/journal.pone.0035742 ◽

2012 ◽

Vol 7 (4) ◽

pp. e35742 ◽

Cited By ~ 52

Author(s):

Wenzel Kröber ◽

Martin Böhnke ◽

Erik Welk ◽

Christian Wirth ◽

Helge Bruelheide

Keyword(s):

East China ◽

Leaf Trait

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Genetic and functional leaf trait diversity throughout the distribution of two Cerrado tree species: Testing the centre‐periphery hypothesis

Journal of Biogeography ◽

10.1111/jbi.14148 ◽

2021 ◽

Author(s):

Thais R. Pfeilsticker ◽

Renata S. O. Buzatti ◽

André C. Muniz ◽

Marcelo L. Bueno ◽

José P. Lemos‐Filho ◽

...

Keyword(s):

Tree Species ◽

Leaf Trait ◽

Trait Diversity

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High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale

Biologia Plantarum ◽

10.1007/s10535-009-0141-9 ◽

2009 ◽

Vol 53 (4) ◽

pp. 769-773 ◽

Cited By ~ 8

Author(s):

X. G. Dai ◽

X. P. Shi ◽

Y. M. Ye ◽

Q. Fu ◽

M. Z. Bao

Keyword(s):

Plant Regeneration ◽

High Frequency ◽

Hypocotyl Explants ◽

Ornamental Kale

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Including leaf trait information helps empirical estimation of jmax from vcmax in cool-temperate deciduous forests

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2021.06.055 ◽

2021 ◽

Author(s):

Guangman Song ◽

Quan Wang ◽

Jia Jin

Keyword(s):

Deciduous Forests ◽

Empirical Estimation ◽

Leaf Trait ◽

Cool Temperate ◽

Temperate Deciduous ◽

Temperate Deciduous Forests

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Differential impact of liana colonization on the leaf functional traits of co-occurring deciduous and evergreen trees

10.21203/rs.3.rs-760614/v1 ◽

2021 ◽

Author(s):

vivek pandi ◽

Kanda Naveen Babu

Keyword(s):

Functional Traits ◽

Tree Species ◽

Dry Matter Content ◽

Differential Impact ◽

Deciduous Species ◽

Evergreen Species ◽

Leaf Functional Traits ◽

Leaf Trait ◽

Host Trees ◽

Significant Difference

Abstract The present study was carried out to analyse the leaf functional traits of co-occurring evergreen and deciduous tree species in a tropical dry scrub forest. This study also intended to check whether the species with contrasting leaf habits differ in their leaf trait plasticity, responding to the canopy-infestation by lianas. A total of 12 leaf functional traits were studied for eight tree species with contrasting leaf habits (evergreen and deciduous) and liana-colonization status (Liana+ and Liana−). In the liana-free environment (L−), evergreen trees had significantly higher specific leaf mass (LMA) and leaf dry matter content (LDMC) than the deciduous species. Whereas, the deciduous trees had higher specific leaf area (SLA) and mass-based leaf nitrogen concentration (Nmass). The leaf trait-pair relationship in the present study agreed to the well-established global trait-pair relationships (SLA Vs Nmass, Lth Vs SLA, Nmass Vs Lth, Nmass Vs LDMC, LDMC Vs SLA). There was no significant difference between L+ and L− individuals in any leaf functional traits studied in the deciduous species. However, evergreen species showed marked differences in the total chlorophyll content (Chlt), chlorophyll b (Chlb), SLA, and LMA between L+ and L− individuals of the same species. Deciduous species with the acquisitive strategy can have a competitive advantage over evergreen species in the exposed environment (L−) whereas, evergreen species with shade-tolerant properties were better acclimated to the shaded environments (L+). The result revealed the patterns of convergence and divergence in some of the leaf functional traits between evergreen and deciduous species. The results also showed the differential impact of liana colonization on the host trees with contrasting leaf habits. Therefore, liana colonization can significantly impact the C-fixation strategies of the host trees by altering their light environment. Further, the magnitude of such impact may vary among species of different leaf habits. The increased proliferation of lianas in the tropical forest canopies may pose a severe threat to the whole forest carbon assimilation rates.

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