Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala)

Abstract Background Leaf shape is an important agronomic trait in ornamental kale (Brassica oleracea L. var. acephala). Although some leaf shape-related genes have been reported in ornamental kale, the detailed mechanism underlying leaf shape formation is still unclear. Here, we report a lobed-leaf trait in ornamental kale, aiming to analyze its inheritance and identify the strong candidate gene. Results Genetic analysis of F2 and BC1 populations demonstrate that the lobed-leaf trait in ornamental kale is controlled by a single dominant gene, termed BoLl-1 (Brassica oleracea lobed-leaf). By performing whole-genome resequencing and linkage analyses, the BoLl-1 gene was finely mapped to a 127-kb interval on chromosome C09 flanked by SNP markers SL4 and SL6, with genetic distances of 0.6 cM and 0.6 cM, respectively. Based on annotations of the genes within this interval, Bo9g181710, an orthologous gene of LATE MERISTEM IDENTITY 1 (LMI1) in Arabidopsis, was predicted as the candidate for BoLl-1, and was renamed BoLMI1a. The expression level of BoLMI1a in lobed-leaf parent 18Q2513 was significantly higher compared with unlobed-leaf parent 18Q2515. Sequence analysis of the parental alleles revealed no sequence variations in the coding sequence of BoLMI1a, whereas a 1737-bp deletion, a 92-bp insertion and an SNP were identified within the BoLMI1a promoter region of parent 18Q2513. Verification analyses with BoLMI1a-specific markers corresponding to the promoter variations revealed that the variations were present only in the lobed-leaf ornamental kale inbred lines. Conclusions This study identified a lobed-leaf gene BoLMI1a, which was fine-mapped to a 127-kb fragment. Three variations were identified in the promoter region of BoLMI1a. The transcription level of BoLMI1a between the two parents exhibited great difference, providing new insight into the molecular mechanism underlying leaf shape formation in ornamental kale.

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Pigment variation and transcriptional response of the pigment synthesis pathway in the S2309 triple-color ornamental kale (Brassica oleracea L. var. acephala) line

Genomics ◽

10.1016/j.ygeno.2020.02.019 ◽

2020 ◽

Vol 112 (3) ◽

pp. 2658-2665

Author(s):

Xiaoping Liu ◽

Bin Zhang ◽

Jie Wu ◽

Zhiyuan Li ◽

Fengqing Han ◽

...

Keyword(s):

Brassica Oleracea ◽

Transcriptional Response ◽

Pigment Synthesis ◽

Ornamental Kale ◽

Pigment Variation ◽

Brassica Oleracea L

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Fine-mapping of a gene for the lobed leaf, BoLl, in ornamental kale (Brassica oleracea L. var. acephala)

Molecular Breeding ◽

10.1007/s11032-019-0944-0 ◽

2019 ◽

Vol 39 (3) ◽

Cited By ~ 6

Author(s):

Jie Ren ◽

Zhiyong Liu ◽

Jiangtao Du ◽

Wei Fu ◽

Ailin Hou ◽

...

Keyword(s):

Brassica Oleracea ◽

Fine Mapping ◽

Ornamental Kale ◽

Lobed Leaf ◽

Brassica Oleracea L

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High frequency plant regeneration from microspore-derived embryos of ornamental kale (Brassica oleracea L. var. acephala)

Scientia Horticulturae ◽

10.1016/j.scienta.2011.06.029 ◽

2011 ◽

Vol 130 (1) ◽

pp. 296-302 ◽

Cited By ~ 15

Author(s):

Yushu Wang ◽

Yan Tong ◽

Yuefei Li ◽

Yun Zhang ◽

Jun Zhang ◽

...

Keyword(s):

Plant Regeneration ◽

Brassica Oleracea ◽

High Frequency ◽

Ornamental Kale ◽

Brassica Oleracea L

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Mapping of Pi, a gene conferring pink leaf in ornamental kale (Brassica oleracea L. var. acephala DC)

Euphytica ◽

10.1007/s10681-015-1555-4 ◽

2015 ◽

Vol 207 (2) ◽

pp. 377-385 ◽

Cited By ~ 8

Author(s):

Pengfang Zhu ◽

Mingming Cheng ◽

Xin Feng ◽

Yong Xiong ◽

Chang Liu ◽

...

Keyword(s):

Brassica Oleracea ◽

Ornamental Kale ◽

Brassica Oleracea L

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Promoter Variations Strongly Enhance the Transcription Level of the BoLMI1 Gene, Causing Lobed Leaves in Ornamental Kale (Brassica Oleracea L. Var. Acephala)

10.21203/rs.3.rs-512886/v1 ◽

2021 ◽

Author(s):

Bin Zhang ◽

Wendi Chen ◽

Xing Li ◽

Wenjing Ren ◽

Li Chen ◽

...

Keyword(s):

Brassica Oleracea ◽

Shape Change ◽

Leaf Shape ◽

Dominant Gene ◽

Snp Markers ◽

Transcription Level ◽

Leaf Trait ◽

Shape Formation ◽

Ornamental Kale ◽

Lobed Leaf

Abstract Background: Leaf shape is an important agronomic trait in ornamental kale (Brassica oleracea L. var. acephala). Although some leaf shape-related genes have been reported in ornamental kale, the detailed mechanism underlying leaf shape formation is still unclear. Here, we report a lobed-leaf trait in ornamental kale, aiming to analyze its inheritance and identify the strong candidate gene.Results: Genetic analysis of F2 and BC1 populations demonstrate that the lobed-leaf trait in ornamental kale is controlled by a single dominant gene, termed BoLl-1. By performing whole-genome resequencing and linkage analyses, the BoLl-1 gene was finely mapped to a 127-kb interval on chromosome C09 flanked by SNP markers SL4 and SL6, with genetic distances of 0.6 cM and 0.6 cM, respectively. Based on annotations of the genes within this interval, Bo9g181710, an orthologous gene of LATE MERISTEM IDENTITY 1 (LMI1) in Arabidopsis, was predicted as the candidate for BoLl-1, and was renamed BoLMI1. The expression level of BoLMI1 in lobed-leaf parent 18Q2513 was significantly higher compared with unlobed-leaf parent 18Q2515. Sequence analysis of the parental alleles revealed no sequence variations in the coding sequence of BoLMI1, whereas a 1737-bp deletion, a 92-bp insertion and an SNP were identified within the BoLMI1 promoter region of parent 18Q2513. Verification analyses with BoLMI1-specific markers corresponding to the promoter variations revealed that the variations were present only in the lobed-leaf ornamental kale inbred lines.Conclusions: This study demonstrated that promoter variations strongly enhance the transcription level of BoLMI1 and cause the leaf shape change from unlobed to lobed, providing new insight into the molecular mechanism underlying leaf shape formation in ornamental kale.

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Calcium nitrate on growth and ornamental traits at salt-stressed condition in ornamental kale (Brassica oleracea L. var. Acephala)

Ornamental Horticulture ◽

10.1590/2447-536x.v27i2.2246 ◽

2021 ◽

Vol 27 (2) ◽

pp. 196-203

Author(s):

Fazilet Parlakova Karagöz ◽

Atilla Dursun

Keyword(s):

Salt Stress ◽

Plant Growth ◽

Brassica Oleracea ◽

Ornamental Plants ◽

Calcium Nitrate ◽

Ion Leakage ◽

Negative Effects ◽

Leaf Tissues ◽

Ornamental Kale ◽

Brassica Oleracea L

Abstract Ornamental plants are used to improve the aestheticquality of urban and rural landscapes, recreational areas, interiorscapes and commercial sites. Any negative effects of salts on ornamental plant growth have to be taken into consideration mainly for their negative influences on aesthetic value of ornamental plants. The research was conducted to determine the effects of different concentrations of calcium nitrate on some stress parameters (i.e. leaf relative water content, chlorophyll, stomatal conductance, ion leakage in the leaf tissues) and some plant growth parameters (total leaf number, number of dried leaf, main stem diameter, plant crown diameter, plant height, fresh and dry weight of root) of ornamental kale (Brassica oleracea L. var. acephala) plant grown under saline conditions. Four doses of NaCl (NaCl1:30, NaCl2:60, NaCl3:90, NaCl4:120 mM), three doses of Ca(NO3)2 (CaN1:5, CaN2:10, CaN3:15 mM), groups combined together in all doses of NaCl and Ca (NO3)2 and control (NaCl0:0 + CaN0:0) were applied into the pots with three replications. Ca (NO3)2 was applied to the leaf surface of the plants in the pots to be applied as Ca (NO3)2 spray. When the plants reached a certain maturity (two weeks after transfer to the pot), salt stress applications were started. Ca (NO3)2 treatments have applied after one week from salt stress applications. Calcium nitrate treatements applied under salt stress conditions had positive effects and increasing in effect on all parameters examined except for chlorophyll and ion leakage in leaf tissues of the plant. With increasing in calcium nitrate concentration, morphological and physiological characteristics of plants were observed increases in general. It was concluded that the increase of the number of leaves by NaCl2+CaN3 application can be minimized with CaN3 (with 15 mM Ca (NO3)2) stress caused by 60 mM salt concentration According to the results of the research, calcium nitrate applications can be recommended for ornamental kale plants grown under salt stress conditions especially limiting the negative effects of stress and to minimize such damage.

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